HIPHOP chemogenomics database
YLR313C / SPH1 
Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YLR313C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.120 | 2.69E-12 | YKL019W | RAM2 | het | Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor |
| 0.116 | 1.44E-11 | YGL155W | CDC43 | het | Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis |
| 0.106 | 6.65E-10 | YLR230W_d | YLR230W_d | het | Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity |
| 0.104 | 1.63E-9 | YOL130W | ALR1 | het | Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions |
| 0.095 | 3.81E-8 | YKL058W | TOA2 | het | TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress |
| 0.092 | 1.01E-7 | YLR229C | CDC42 | het | Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins |
| 0.091 | 1.32E-7 | YLR153C | ACS2 | het | Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions |
| 0.088 | 3.15E-7 | YER154W | OXA1 | hom | Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals |
| 0.086 | 6.41E-7 | YGR071C | ENV11 | hom | Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus |
| 0.085 | 9.70E-7 | YDR309C | GIC2 | hom | Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region |
| 0.080 | 3.03E-6 | YPR032W | SRO7 | hom | Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication |
| 0.080 | 3.84E-6 | YDR019C | GCV1 | hom | T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm |
| 0.079 | 4.03E-6 | YBR200W | BEM1 | hom | Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p |
| 0.079 | 4.87E-6 | YLR306W | UBC12 | hom | Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes |
| 0.075 | 1.47E-5 | YMR241W | YHM2 | hom | Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome |