YLR313C / SPH1

Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR313C / SPH1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.64|P-value:2.20E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:3.94|P-value:7.13E-5 ID:SGTC_88|Compound:0180-0332|FD-Score:5.34|P-value:1.09E-7 ID:SGTC_92|Compound:0063-0024|FD-Score:4.11|P-value:3.49E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.53|P-value:3.34E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.66|P-value:2.05E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.26|P-value:5.75E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:-3.70|P-value:1.13E-4 ID:SGTC_389|Compound:tpck|FD-Score:3.21|P-value:9.92E-4 ID:SGTC_459|Compound:nigericin|FD-Score:4.71|P-value:2.59E-6 ID:SGTC_656|Compound:4090-1979|FD-Score:3.58|P-value:2.81E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.32|P-value:7.02E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:3.97|P-value:6.29E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.25|P-value:8.90E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.22|P-value:9.70E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.65|P-value:2.13E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.49|P-value:3.84E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.24|P-value:8.97E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.15|P-value:8.20E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.37|P-value:5.78E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.44|P-value:2.96E-4 ID:SGTC_1526|Compound:st051518|FD-Score:4.92|P-value:9.44E-7 ID:SGTC_1693|Compound:xanthone|FD-Score:3.36|P-value:6.01E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.93|P-value:7.37E-5 ID:SGTC_1746|Compound:st038117|FD-Score:4.73|P-value:2.27E-6 ID:SGTC_1831|Compound:st055328|FD-Score:3.32|P-value:6.85E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.90|P-value:8.33E-5 ID:SGTC_1909|Compound:st060837|FD-Score:3.45|P-value:4.35E-4 ID:SGTC_2122|Compound:5258151|FD-Score:3.26|P-value:8.39E-4 ID:SGTC_2125|Compound:5246583|FD-Score:-3.72|P-value:1.06E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.12|P-value:9.17E-4 ID:SGTC_2173|Compound:5763948|FD-Score:3.23|P-value:9.35E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.26|P-value:8.53E-4 ID:SGTC_2182|Compound:6325192|FD-Score:3.44|P-value:4.50E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.32|P-value:6.92E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.40|P-value:5.27E-4 ID:SGTC_2351|Compound:9026858|FD-Score:3.21|P-value:9.92E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.60|P-value:2.60E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.58|P-value:1.76E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.31|P-value:7.24E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.34|P-value:1.36E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.41|P-value:5.13E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.84|P-value:1.03E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.48|P-value:7.41E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:5.99|P-value:3.02E-9 ID:SGTC_2694|Compound:st077685|FD-Score:4.35|P-value:1.26E-5 ID:SGTC_2809|Compound:7964133|FD-Score:-3.18|P-value:7.56E-4 ID:SGTC_2882|Compound:9058044|FD-Score:-4.22|P-value:1.35E-5 ID:SGTC_2887|Compound:9044784|FD-Score:4.32|P-value:1.46E-5 ID:SGTC_2924|Compound:9010168|FD-Score:8.87|P-value:2.80E-18 ID:SGTC_2945|Compound:9067681|FD-Score:3.72|P-value:1.62E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.52|P-value:3.40E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.27|P-value:8.26E-4 ID:SGTC_3026|Compound:9090633|FD-Score:-3.26|P-value:5.71E-4 ID:SGTC_3239|Compound:9133757|FD-Score:3.57|P-value:2.86E-4 ID:SGTC_3304|Compound:9125678|FD-Score:5.39|P-value:8.44E-8 ID:SGTC_3306|Compound:9127676|FD-Score:3.86|P-value:9.53E-5 ID:SGTC_3318|Compound:9139014|FD-Score:-3.84|P-value:6.63E-5 ID:SGTC_3331|Compound:9141060|FD-Score:3.42|P-value:4.96E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.64|P-value:2.20E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:3.94|P-value:7.13E-5 ID:SGTC_88|Compound:0180-0332|FD-Score:5.34|P-value:1.09E-7 ID:SGTC_92|Compound:0063-0024|FD-Score:4.11|P-value:3.49E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:3.53|P-value:3.34E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.66|P-value:2.05E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.26|P-value:5.75E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:-3.70|P-value:1.13E-4 ID:SGTC_389|Compound:tpck|FD-Score:3.21|P-value:9.92E-4 ID:SGTC_459|Compound:nigericin|FD-Score:4.71|P-value:2.59E-6 ID:SGTC_656|Compound:4090-1979|FD-Score:3.58|P-value:2.81E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.32|P-value:7.02E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:3.97|P-value:6.29E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.25|P-value:8.90E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.22|P-value:9.70E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.65|P-value:2.13E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.49|P-value:3.84E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.24|P-value:8.97E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.15|P-value:8.20E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.37|P-value:5.78E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.44|P-value:2.96E-4 ID:SGTC_1526|Compound:st051518|FD-Score:4.92|P-value:9.44E-7 ID:SGTC_1693|Compound:xanthone|FD-Score:3.36|P-value:6.01E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.93|P-value:7.37E-5 ID:SGTC_1746|Compound:st038117|FD-Score:4.73|P-value:2.27E-6 ID:SGTC_1831|Compound:st055328|FD-Score:3.32|P-value:6.85E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.90|P-value:8.33E-5 ID:SGTC_1909|Compound:st060837|FD-Score:3.45|P-value:4.35E-4 ID:SGTC_2122|Compound:5258151|FD-Score:3.26|P-value:8.39E-4 ID:SGTC_2125|Compound:5246583|FD-Score:-3.72|P-value:1.06E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.12|P-value:9.17E-4 ID:SGTC_2173|Compound:5763948|FD-Score:3.23|P-value:9.35E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.26|P-value:8.53E-4 ID:SGTC_2182|Compound:6325192|FD-Score:3.44|P-value:4.50E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.32|P-value:6.92E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.40|P-value:5.27E-4 ID:SGTC_2351|Compound:9026858|FD-Score:3.21|P-value:9.92E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.60|P-value:2.60E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.58|P-value:1.76E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.31|P-value:7.24E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.34|P-value:1.36E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.41|P-value:5.13E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.84|P-value:1.03E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.48|P-value:7.41E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:5.99|P-value:3.02E-9 ID:SGTC_2694|Compound:st077685|FD-Score:4.35|P-value:1.26E-5 ID:SGTC_2809|Compound:7964133|FD-Score:-3.18|P-value:7.56E-4 ID:SGTC_2882|Compound:9058044|FD-Score:-4.22|P-value:1.35E-5 ID:SGTC_2887|Compound:9044784|FD-Score:4.32|P-value:1.46E-5 ID:SGTC_2924|Compound:9010168|FD-Score:8.87|P-value:2.80E-18 ID:SGTC_2945|Compound:9067681|FD-Score:3.72|P-value:1.62E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.52|P-value:3.40E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.27|P-value:8.26E-4 ID:SGTC_3026|Compound:9090633|FD-Score:-3.26|P-value:5.71E-4 ID:SGTC_3239|Compound:9133757|FD-Score:3.57|P-value:2.86E-4 ID:SGTC_3304|Compound:9125678|FD-Score:5.39|P-value:8.44E-8 ID:SGTC_3306|Compound:9127676|FD-Score:3.86|P-value:9.53E-5 ID:SGTC_3318|Compound:9139014|FD-Score:-3.84|P-value:6.63E-5 ID:SGTC_3331|Compound:9141060|FD-Score:3.42|P-value:4.96E-4

Top fitness defect scores for YLR313C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2924 9010168 8.87 62.8 µM 2.80E-18 cell wall signaling 3 3.70E-19
2 SGTC_2595 hexamethylquercetagetin 5.99 100.0 µM 3.02E-9 5 1.06E-9
3 SGTC_3304 9125678 5.39 46.1 µM 8.44E-8 18 3.47E-8
4 SGTC_88 0180-0332 5.34 8.7 µM 1.09E-7 2 4.54E-8
5 SGTC_1526 st051518 4.92 75.0 µM 9.44E-7 7 4.38E-7
6 SGTC_1746 st038117 4.73 42.0 µM 2.27E-6 17 1.10E-6
7 SGTC_459 nigericin 4.71 15.3 µM 2.59E-6 49 1.26E-6
8 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 4.48 100.0 µM 7.41E-6 60S ribosome export 12 3.81E-6
9 SGTC_2694 st077685 4.35 81.9 µM 1.26E-5 endomembrane recycling 19 6.66E-6
10 SGTC_2513 baicalein 4.34 25.6 µM 1.36E-5 calcium & mitochondrial duress 20 7.19E-6
11 SGTC_2887 9044784 4.32 58.4 µM 1.46E-5 fatty acid desaturase (OLE1) 22 7.78E-6
12 SGTC_92 0063-0024 4.11 660.9 µM 3.49E-5 8 1.94E-5
13 SGTC_946 3434-1334 3.97 87.4 µM 6.29E-5 31 3.61E-5
14 SGTC_10 0279-0212 3.94 42.6 µM 7.13E-5 TRP & mitochondrial translation 124 4.12E-5
15 SGTC_1728 st035614 3.93 33.8 µM 7.37E-5 51 4.27E-5
16 SGTC_1870 st057514 3.90 26.5 µM 8.33E-5 RSC & ERG11 23 4.85E-5
17 SGTC_3306 9127676 3.86 59.8 µM 9.53E-5 25 5.59E-5
18 SGTC_2559 tetrahydrosappanone a trimethyl ether 3.84 100.0 µM 1.03E-4 19 6.05E-5
19 SGTC_2945 9067681 3.72 61.8 µM 1.62E-4 18 9.79E-5
20 SGTC_116 0155-0182 3.66 31.8 µM 2.05E-4 mitochondrial response to ROS 103 1.25E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.120 2.69E-12 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.116 1.44E-11 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.106 6.65E-10 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.104 1.63E-9 YOL130W ALR1 het Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
0.095 3.81E-8 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.092 1.01E-7 YLR229C CDC42 het Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
0.091 1.32E-7 YLR153C ACS2 het Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
0.088 3.15E-7 YER154W OXA1 hom Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals
0.086 6.41E-7 YGR071C ENV11 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
0.085 9.70E-7 YDR309C GIC2 hom Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region
0.080 3.03E-6 YPR032W SRO7 hom Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
0.080 3.84E-6 YDR019C GCV1 hom T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
0.079 4.03E-6 YBR200W BEM1 hom Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
0.079 4.87E-6 YLR306W UBC12 hom Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes
0.075 1.47E-5 YMR241W YHM2 hom Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome