YLR315W / NKP2

Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR315W / NKP2

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-3.66|P-value:2.43E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:-3.37|P-value:6.36E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:5.48|P-value:2.85E-7 ID:SGTC_264|Compound:promethazine|FD-Score:3.52|P-value:7.24E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-5.58|P-value:6.87E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-14.10|P-value:5.75E-40 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.71|P-value:4.00E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.74|P-value:7.95E-6 ID:SGTC_688|Compound:0527-0193|FD-Score:3.96|P-value:1.64E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.58|P-value:3.19E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.61|P-value:1.35E-5 ID:SGTC_974|Compound:1326-0144|FD-Score:5.71|P-value:9.18E-8 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.45|P-value:9.18E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.48|P-value:4.42E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.75|P-value:1.75E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.51|P-value:7.46E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:7.92|P-value:1.81E-13 ID:SGTC_1369|Compound:1866-0067|FD-Score:6.07|P-value:1.45E-8 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.11|P-value:9.48E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.61|P-value:1.40E-5 ID:SGTC_1619|Compound:st003326|FD-Score:-3.79|P-value:1.52E-4 ID:SGTC_2024|Compound:5144227|FD-Score:4.42|P-value:2.91E-5 ID:SGTC_2292|Compound:7671323|FD-Score:3.66|P-value:4.67E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.49|P-value:2.19E-5 ID:SGTC_2467|Compound:5474540|FD-Score:-3.51|P-value:4.00E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:4.55|P-value:1.74E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:6.81|P-value:2.23E-10 ID:SGTC_2877|Compound:9040724|FD-Score:3.43|P-value:9.69E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-4.22|P-value:3.00E-5 ID:SGTC_2952|Compound:9073731|FD-Score:4.41|P-value:3.11E-5 ID:SGTC_2964|Compound:9083345|FD-Score:-3.48|P-value:4.41E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.92|P-value:1.59E-6 ID:SGTC_3189|Compound:9110682|FD-Score:4.08|P-value:1.08E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.12|P-value:9.09E-5 ID:SGTC_3328|Compound:9140624|FD-Score:-3.76|P-value:1.72E-4 ID:SGTC_3334|Compound:9142812|FD-Score:5.68|P-value:1.07E-7 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.66|P-value:2.43E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:-3.37|P-value:6.36E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:5.48|P-value:2.85E-7 ID:SGTC_264|Compound:promethazine|FD-Score:3.52|P-value:7.24E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-5.58|P-value:6.87E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-14.10|P-value:5.75E-40 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.71|P-value:4.00E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.74|P-value:7.95E-6 ID:SGTC_688|Compound:0527-0193|FD-Score:3.96|P-value:1.64E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.58|P-value:3.19E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.61|P-value:1.35E-5 ID:SGTC_974|Compound:1326-0144|FD-Score:5.71|P-value:9.18E-8 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.45|P-value:9.18E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.48|P-value:4.42E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.75|P-value:1.75E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.51|P-value:7.46E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:7.92|P-value:1.81E-13 ID:SGTC_1369|Compound:1866-0067|FD-Score:6.07|P-value:1.45E-8 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.11|P-value:9.48E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.61|P-value:1.40E-5 ID:SGTC_1619|Compound:st003326|FD-Score:-3.79|P-value:1.52E-4 ID:SGTC_2024|Compound:5144227|FD-Score:4.42|P-value:2.91E-5 ID:SGTC_2292|Compound:7671323|FD-Score:3.66|P-value:4.67E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.49|P-value:2.19E-5 ID:SGTC_2467|Compound:5474540|FD-Score:-3.51|P-value:4.00E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:4.55|P-value:1.74E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:6.81|P-value:2.23E-10 ID:SGTC_2877|Compound:9040724|FD-Score:3.43|P-value:9.69E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-4.22|P-value:3.00E-5 ID:SGTC_2952|Compound:9073731|FD-Score:4.41|P-value:3.11E-5 ID:SGTC_2964|Compound:9083345|FD-Score:-3.48|P-value:4.41E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.92|P-value:1.59E-6 ID:SGTC_3189|Compound:9110682|FD-Score:4.08|P-value:1.08E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.12|P-value:9.09E-5 ID:SGTC_3328|Compound:9140624|FD-Score:-3.76|P-value:1.72E-4 ID:SGTC_3334|Compound:9142812|FD-Score:5.68|P-value:1.07E-7

Top fitness defect scores for YLR315W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1368 1852-0141 7.92 284.0 µM 1.81E-13 ERG2 2 1.18E-15
2 SGTC_2750 bromebric acid 6.81 70.2 µM 2.23E-10 2 4.89E-12
3 SGTC_1369 1866-0067 6.07 208.0 µM 1.45E-8 RPP1 & pyrimidine depletion 10 6.52E-10
4 SGTC_974 1326-0144 5.71 138.0 µM 9.18E-8 ERG2 12 5.67E-9
5 SGTC_3334 9142812 5.68 21.9 µM 1.07E-7 6 6.79E-9
6 SGTC_161 4065-0411 5.48 162.2 µM 2.85E-7 36 2.14E-8
7 SGTC_682 0kpi-0099 4.74 17.0 µM 7.95E-6 43 1.06E-6
8 SGTC_920 mycophenolic acid 4.61 780.4 nM 1.35E-5 16 1.98E-6
9 SGTC_1373 2887-0003 4.61 58.5 µM 1.40E-5 Golgi 11 2.06E-6
10 SGTC_2595 hexamethylquercetagetin 4.55 100.0 µM 1.74E-5 13 2.66E-6
11 SGTC_2293 7624732 4.49 200.0 µM 2.19E-5 17 3.49E-6
12 SGTC_2024 5144227 4.42 134.0 µM 2.91E-5 26 4.87E-6
13 SGTC_2952 9073731 4.41 13.8 µM 3.11E-5 tubulin folding & SWR complex 24 5.27E-6
14 SGTC_3323 9138564 4.12 53.9 µM 9.09E-5 18 1.85E-5
15 SGTC_1372 2752-0194 4.11 270.0 µM 9.48E-5 13 1.95E-5
16 SGTC_3189 9110682 4.08 49.5 µM 1.08E-4 33 2.28E-5
17 SGTC_688 0527-0193 3.96 473.0 µM 1.64E-4 fatty acid desaturase (OLE1) 13 3.72E-5
18 SGTC_2292 7671323 3.66 168.1 µM 4.67E-4 RNA processing & uracil transport 42 1.27E-4
19 SGTC_264 promethazine 3.52 12.5 mM 7.24E-4 DNA intercalators 71 2.12E-4
20 SGTC_1189 1431-2119 3.51 7.3 µM 7.46E-4 44 2.20E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.228 8.35E-41 YOR073W SGO1 hom Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
0.211 9.58E-35 YFL001W DEG1 hom tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
0.184 9.68E-27 YAL059W ECM1 hom Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
0.178 3.99E-25 YAL047C SPC72 hom Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization
0.174 2.98E-24 YPL011C TAF3 het TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.162 3.88E-21 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.162 5.58E-21 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.159 2.14E-20 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.146 2.12E-17 YGR160W_d YGR160W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.144 6.08E-17 YNL297C MON2 hom Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins
0.143 8.74E-17 YML123C PHO84 hom High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
0.140 4.08E-16 YPR036W VMA13 hom Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
0.130 3.92E-14 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.130 5.11E-14 YBR173C UMP1 hom Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
0.129 5.96E-14 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W