YLR318W / EST2

Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR318W / EST2

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:-3.07|P-value:9.67E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.40|P-value:4.20E-6 ID:SGTC_123|Compound:0083-0120|FD-Score:3.19|P-value:6.11E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:5.40|P-value:2.38E-8 ID:SGTC_138|Compound:0850-0259|FD-Score:5.75|P-value:2.98E-9 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.09|P-value:9.19E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.17|P-value:7.01E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.43|P-value:2.62E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:3.13|P-value:7.51E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:3.12|P-value:7.97E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:4.36|P-value:5.08E-6 ID:SGTC_802|Compound:4112-3352|FD-Score:5.02|P-value:1.85E-7 ID:SGTC_839|Compound:0122-0028|FD-Score:3.64|P-value:1.16E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.54|P-value:1.79E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.23|P-value:5.63E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.13|P-value:7.55E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:4.88|P-value:3.99E-7 ID:SGTC_1017|Compound:4239-0030|FD-Score:4.77|P-value:6.95E-7 ID:SGTC_1148|Compound:3909-7663|FD-Score:4.08|P-value:1.82E-5 ID:SGTC_1168|Compound:k815-0004|FD-Score:4.93|P-value:3.00E-7 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.15|P-value:7.04E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:4.38|P-value:4.55E-6 ID:SGTC_1289|Compound:0976-0073|FD-Score:5.41|P-value:2.22E-8 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.13|P-value:7.57E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.25|P-value:8.45E-6 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.26|P-value:4.76E-4 ID:SGTC_1765|Compound:st044815|FD-Score:4.22|P-value:9.84E-6 ID:SGTC_1870|Compound:st057514|FD-Score:4.38|P-value:4.67E-6 ID:SGTC_1931|Compound:st074438|FD-Score:3.05|P-value:9.98E-4 ID:SGTC_2062|Compound:5245942|FD-Score:3.95|P-value:3.13E-5 ID:SGTC_2162|Compound:5663267|FD-Score:-3.97|P-value:3.14E-5 ID:SGTC_2168|Compound:5727979|FD-Score:3.10|P-value:8.36E-4 ID:SGTC_2210|Compound:7115885|FD-Score:7.67|P-value:4.32E-15 ID:SGTC_2240|Compound:6690706|FD-Score:3.48|P-value:2.12E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.08|P-value:9.12E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.30|P-value:4.19E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.97|P-value:2.52E-7 ID:SGTC_2385|Compound:9074692|FD-Score:-3.33|P-value:3.83E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.13|P-value:7.61E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-4.37|P-value:5.22E-6 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-3.07|P-value:9.84E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:4.20|P-value:1.06E-5 ID:SGTC_2783|Compound:7738165|FD-Score:4.34|P-value:5.68E-6 ID:SGTC_2903|Compound:5923464|FD-Score:3.46|P-value:2.27E-4 ID:SGTC_2986|Compound:9022445|FD-Score:-3.12|P-value:8.17E-4 ID:SGTC_2996|Compound:9066192|FD-Score:3.45|P-value:2.35E-4 ID:SGTC_3001|Compound:9076041|FD-Score:3.78|P-value:6.50E-5 ID:SGTC_3212|Compound:9128307|FD-Score:4.94|P-value:2.87E-7 ID:SGTC_3262|Compound:9137218|FD-Score:-3.08|P-value:9.42E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:-3.07|P-value:9.67E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.40|P-value:4.20E-6 ID:SGTC_123|Compound:0083-0120|FD-Score:3.19|P-value:6.11E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:5.40|P-value:2.38E-8 ID:SGTC_138|Compound:0850-0259|FD-Score:5.75|P-value:2.98E-9 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.09|P-value:9.19E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.17|P-value:7.01E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.43|P-value:2.62E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:3.13|P-value:7.51E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:3.12|P-value:7.97E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:4.36|P-value:5.08E-6 ID:SGTC_802|Compound:4112-3352|FD-Score:5.02|P-value:1.85E-7 ID:SGTC_839|Compound:0122-0028|FD-Score:3.64|P-value:1.16E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.54|P-value:1.79E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.23|P-value:5.63E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.13|P-value:7.55E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:4.88|P-value:3.99E-7 ID:SGTC_1017|Compound:4239-0030|FD-Score:4.77|P-value:6.95E-7 ID:SGTC_1148|Compound:3909-7663|FD-Score:4.08|P-value:1.82E-5 ID:SGTC_1168|Compound:k815-0004|FD-Score:4.93|P-value:3.00E-7 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.15|P-value:7.04E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:4.38|P-value:4.55E-6 ID:SGTC_1289|Compound:0976-0073|FD-Score:5.41|P-value:2.22E-8 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.13|P-value:7.57E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.25|P-value:8.45E-6 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.26|P-value:4.76E-4 ID:SGTC_1765|Compound:st044815|FD-Score:4.22|P-value:9.84E-6 ID:SGTC_1870|Compound:st057514|FD-Score:4.38|P-value:4.67E-6 ID:SGTC_1931|Compound:st074438|FD-Score:3.05|P-value:9.98E-4 ID:SGTC_2062|Compound:5245942|FD-Score:3.95|P-value:3.13E-5 ID:SGTC_2162|Compound:5663267|FD-Score:-3.97|P-value:3.14E-5 ID:SGTC_2168|Compound:5727979|FD-Score:3.10|P-value:8.36E-4 ID:SGTC_2210|Compound:7115885|FD-Score:7.67|P-value:4.32E-15 ID:SGTC_2240|Compound:6690706|FD-Score:3.48|P-value:2.12E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.08|P-value:9.12E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.30|P-value:4.19E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.97|P-value:2.52E-7 ID:SGTC_2385|Compound:9074692|FD-Score:-3.33|P-value:3.83E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.13|P-value:7.61E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-4.37|P-value:5.22E-6 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-3.07|P-value:9.84E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:4.20|P-value:1.06E-5 ID:SGTC_2783|Compound:7738165|FD-Score:4.34|P-value:5.68E-6 ID:SGTC_2903|Compound:5923464|FD-Score:3.46|P-value:2.27E-4 ID:SGTC_2986|Compound:9022445|FD-Score:-3.12|P-value:8.17E-4 ID:SGTC_2996|Compound:9066192|FD-Score:3.45|P-value:2.35E-4 ID:SGTC_3001|Compound:9076041|FD-Score:3.78|P-value:6.50E-5 ID:SGTC_3212|Compound:9128307|FD-Score:4.94|P-value:2.87E-7 ID:SGTC_3262|Compound:9137218|FD-Score:-3.08|P-value:9.42E-4

Top fitness defect scores for YLR318W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2210 7115885 7.67 190.4 µM 4.32E-15 cell wall signaling 5 8.68E-15
2 SGTC_138 0850-0259 5.75 40.9 µM 2.98E-9 cell wall 113 4.47E-9
3 SGTC_1289 0976-0073 5.41 69.1 µM 2.22E-8 2 3.19E-8
4 SGTC_136 0097-0015 5.40 404.5 µM 2.38E-8 60S ribosome export 2 3.41E-8
5 SGTC_802 4112-3352 5.02 742.0 µM 1.85E-7 8 2.53E-7
6 SGTC_2293 7624732 4.97 200.0 µM 2.52E-7 6 3.43E-7
7 SGTC_3212 9128307 4.94 49.5 µM 2.87E-7 27 3.90E-7
8 SGTC_1168 k815-0004 4.93 80.5 µM 3.00E-7 DNA damage response 3 4.07E-7
9 SGTC_1011 4112-3381 4.88 17.4 µM 3.99E-7 20 5.38E-7
10 SGTC_1017 4239-0030 4.77 168.0 µM 6.95E-7 10 9.26E-7
11 SGTC_58 0957-0393 4.40 9.0 µM 4.20E-6 cell wall 335 5.39E-6
12 SGTC_1268 0828-0275 4.38 2.5 µM 4.55E-6 14 5.82E-6
13 SGTC_1870 st057514 4.38 26.5 µM 4.67E-6 RSC & ERG11 16 5.97E-6
14 SGTC_797 k781-0471 4.36 270.0 µM 5.08E-6 RPP1 & pyrimidine depletion 10 6.48E-6
15 SGTC_2783 7738165 4.34 71.4 µM 5.68E-6 24 7.24E-6
16 SGTC_1342 1486-1464 4.25 234.0 µM 8.45E-6 10 1.07E-5
17 SGTC_1765 st044815 4.22 90.4 µM 9.84E-6 20 1.24E-5
18 SGTC_2686 alizarin 4.20 95.4 µM 1.06E-5 RPP1 & pyrimidine depletion 27 1.33E-5
19 SGTC_1148 3909-7663 4.08 27.7 µM 1.82E-5 15 2.27E-5
20 SGTC_2062 5245942 3.95 200.0 µM 3.13E-5 19 3.84E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.177 5.88E-25 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.169 5.85E-23 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.169 1.01E-22 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.156 1.17E-19 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.155 1.78E-19 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.151 1.26E-18 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.151 1.51E-18 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.149 4.23E-18 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.144 4.43E-17 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.143 9.00E-17 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.141 2.33E-16 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.141 2.53E-16 YGL060W YBP2 hom Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
0.141 2.54E-16 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.140 3.71E-16 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.140 3.84E-16 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan