YLR319C / BUD6

Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR319C / BUD6

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:6.93|P-value:1.35E-11 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.40|P-value:4.90E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.72|P-value:1.79E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.04|P-value:5.27E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.94|P-value:1.02E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:4.94|P-value:1.04E-6 ID:SGTC_425|Compound:3825-7017|FD-Score:-3.67|P-value:1.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.90|P-value:9.23E-5 ID:SGTC_555|Compound:4296-0685|FD-Score:3.85|P-value:1.12E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.58|P-value:2.95E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.53|P-value:3.57E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.61|P-value:2.71E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.74|P-value:1.68E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.54|P-value:3.39E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.54|P-value:3.02E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.92|P-value:7.37E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.21|P-value:9.28E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:-4.56|P-value:5.13E-6 ID:SGTC_972|Compound:1319-0219|FD-Score:3.80|P-value:1.34E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.22|P-value:9.20E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.44|P-value:4.27E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:-3.54|P-value:3.01E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.91|P-value:8.88E-5 ID:SGTC_1647|Compound:st011722|FD-Score:3.33|P-value:6.95E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.47|P-value:4.45E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.90|P-value:9.00E-5 ID:SGTC_1953|Compound:st076800|FD-Score:3.52|P-value:3.68E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.84|P-value:1.01E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-4.23|P-value:2.15E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.46|P-value:4.08E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-4.18|P-value:2.66E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.29|P-value:7.21E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.16|P-value:3.32E-5 ID:SGTC_3354|Compound:9151612|FD-Score:-3.44|P-value:4.33E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:6.93|P-value:1.35E-11 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.40|P-value:4.90E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.72|P-value:1.79E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.04|P-value:5.27E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.94|P-value:1.02E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:4.94|P-value:1.04E-6 ID:SGTC_425|Compound:3825-7017|FD-Score:-3.67|P-value:1.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.90|P-value:9.23E-5 ID:SGTC_555|Compound:4296-0685|FD-Score:3.85|P-value:1.12E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.58|P-value:2.95E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.53|P-value:3.57E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:3.61|P-value:2.71E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.74|P-value:1.68E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.54|P-value:3.39E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.54|P-value:3.02E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.92|P-value:7.37E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.21|P-value:9.28E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:-4.56|P-value:5.13E-6 ID:SGTC_972|Compound:1319-0219|FD-Score:3.80|P-value:1.34E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.22|P-value:9.20E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.44|P-value:4.27E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:-3.54|P-value:3.01E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.91|P-value:8.88E-5 ID:SGTC_1647|Compound:st011722|FD-Score:3.33|P-value:6.95E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.47|P-value:4.45E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.90|P-value:9.00E-5 ID:SGTC_1953|Compound:st076800|FD-Score:3.52|P-value:3.68E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.84|P-value:1.01E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-4.23|P-value:2.15E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.46|P-value:4.08E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-4.18|P-value:2.66E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.29|P-value:7.21E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.16|P-value:3.32E-5 ID:SGTC_3354|Compound:9151612|FD-Score:-3.44|P-value:4.33E-4

Top fitness defect scores for YLR319C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 6.93 19.8 µM 1.35E-11 6 2.10E-12
2 SGTC_351 caspofungin 4.94 25.0 nM 1.02E-6 cell wall 59 3.81E-7
3 SGTC_354 2783-3500 4.94 4.9 µM 1.04E-6 cell wall 31 3.87E-7
4 SGTC_2987 9018338 4.16 71.4 µM 3.32E-5 RPP1 & pyrimidine depletion 10 1.62E-5
5 SGTC_138 0850-0259 4.04 40.9 µM 5.27E-5 cell wall 195 2.66E-5
6 SGTC_1575 cyproterone acetate 3.91 48.0 µM 8.88E-5 19 4.67E-5
7 SGTC_1747 st037496 3.90 66.6 µM 9.00E-5 19 4.73E-5
8 SGTC_453 enantio-paf c16 3.90 10.0 µM 9.23E-5 148 4.86E-5
9 SGTC_555 4296-0685 3.85 37.0 µM 1.12E-4 ergosterol biosynthesis 9 5.97E-5
10 SGTC_972 1319-0219 3.80 195.0 µM 1.34E-4 34 7.24E-5
11 SGTC_837 0091-0265 3.74 12.5 µM 1.68E-4 ubiquinone biosynthesis & proteasome 29 9.27E-5
12 SGTC_132 0417-1715 3.72 25.8 µM 1.79E-4 RPP1 & pyrimidine depletion 29 9.95E-5
13 SGTC_805 3852-0218 3.61 479.0 µM 2.71E-4 ERAD & cell cycle 84 1.55E-4
14 SGTC_690 glyoxal 3.58 20.3 µM 2.95E-4 99 1.70E-4
15 SGTC_840 0245-0196 3.54 390.0 µM 3.39E-4 22 1.98E-4
16 SGTC_781 4335-2806 3.53 94.4 µM 3.57E-4 33 2.09E-4
17 SGTC_1953 st076800 3.52 12.1 µM 3.68E-4 60 2.16E-4
18 SGTC_1745 st081495 3.47 74.0 µM 4.45E-4 RNA processing & uracil transport 97 2.65E-4
19 SGTC_1647 st011722 3.33 22.4 µM 6.95E-4 22 4.28E-4
20 SGTC_2688 kanamycin b 3.21 96.7 µM 0.00106 calcium & mitochondrial duress 492 6.74E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 2.20E-16 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.141 2.81E-16 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.140 3.36E-16 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.137 1.44E-15 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.135 4.10E-15 YOR315W SFG1 hom Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
0.132 1.55E-14 YPL092W SSU1 hom Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein
0.131 2.41E-14 YGL101W_p YGL101W_p hom Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p
0.130 4.90E-14 YKL201C MNN4 hom Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.129 7.36E-14 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.128 8.79E-14 YKL176C LST4 hom Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
0.128 1.02E-13 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.127 1.40E-13 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.127 1.49E-13 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.126 2.42E-13 YCL021W-A_p YCL021W-A_p hom Putative protein of unknown function
0.126 2.44E-13 YGL027C CWH41 hom Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress