YLR320W / MMS22

Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR320W / MMS22

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.49|P-value:2.38E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.48|P-value:2.34E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:4.69|P-value:1.30E-6 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.16|P-value:7.46E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-4.40|P-value:4.82E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.57|P-value:1.76E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.68|P-value:1.38E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:3.40|P-value:3.30E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.12|P-value:8.83E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.41|P-value:4.96E-6 ID:SGTC_801|Compound:0866-0028|FD-Score:3.48|P-value:2.47E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:3.73|P-value:9.16E-5 ID:SGTC_856|Compound:0433-0016|FD-Score:4.99|P-value:2.76E-7 ID:SGTC_918|Compound:glabranin|FD-Score:3.46|P-value:2.67E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.48|P-value:2.39E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.72|P-value:9.50E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.62|P-value:1.45E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:4.59|P-value:2.12E-6 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.65|P-value:1.21E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.29|P-value:4.85E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:4.45|P-value:4.10E-6 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.13|P-value:8.65E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:5.68|P-value:6.05E-9 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.15|P-value:7.70E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:-3.31|P-value:4.40E-4 ID:SGTC_1728|Compound:st035614|FD-Score:-4.77|P-value:8.26E-7 ID:SGTC_1806|Compound:st050005|FD-Score:3.12|P-value:8.92E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.09|P-value:2.10E-5 ID:SGTC_1863|Compound:st057641|FD-Score:3.61|P-value:1.49E-4 ID:SGTC_2084|Compound:5306078|FD-Score:3.78|P-value:7.47E-5 ID:SGTC_2116|Compound:5511452|FD-Score:4.60|P-value:2.03E-6 ID:SGTC_2183|Compound:6420625|FD-Score:4.35|P-value:6.42E-6 ID:SGTC_2442|Compound:5158906|FD-Score:3.29|P-value:4.92E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.53|P-value:1.99E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-3.63|P-value:1.32E-4 ID:SGTC_2691|Compound:st077356|FD-Score:4.31|P-value:7.74E-6 ID:SGTC_2694|Compound:st077685|FD-Score:3.76|P-value:8.26E-5 ID:SGTC_2695|Compound:st077686|FD-Score:3.97|P-value:3.47E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-4.78|P-value:7.66E-7 ID:SGTC_2780|Compound:5215298|FD-Score:4.74|P-value:9.99E-7 ID:SGTC_2787|Compound:5284540|FD-Score:-3.20|P-value:6.48E-4 ID:SGTC_2853|Compound:9015042|FD-Score:3.88|P-value:5.12E-5 ID:SGTC_2871|Compound:9038510|FD-Score:-3.11|P-value:8.67E-4 ID:SGTC_2981|Compound:9013952|FD-Score:-3.35|P-value:3.72E-4 ID:SGTC_3024|Compound:9090631|FD-Score:4.71|P-value:1.17E-6 ID:SGTC_3029|Compound:9088683|FD-Score:-3.18|P-value:6.97E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-4.16|P-value:1.41E-5 ID:SGTC_3056|Compound:9094900|FD-Score:3.39|P-value:3.38E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.29|P-value:4.95E-4 ID:SGTC_3158|Compound:9100007|FD-Score:4.00|P-value:3.07E-5 ID:SGTC_3196|Compound:9111891|FD-Score:5.04|P-value:2.17E-7 ID:SGTC_3207|Compound:9114163|FD-Score:-4.36|P-value:5.93E-6 ID:SGTC_3212|Compound:9128307|FD-Score:5.50|P-value:1.72E-8 ID:SGTC_3350|Compound:9150499|FD-Score:3.18|P-value:7.20E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.49|P-value:2.38E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.48|P-value:2.34E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:4.69|P-value:1.30E-6 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.16|P-value:7.46E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-4.40|P-value:4.82E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.57|P-value:1.76E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.68|P-value:1.38E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:3.40|P-value:3.30E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.12|P-value:8.83E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.41|P-value:4.96E-6 ID:SGTC_801|Compound:0866-0028|FD-Score:3.48|P-value:2.47E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:3.73|P-value:9.16E-5 ID:SGTC_856|Compound:0433-0016|FD-Score:4.99|P-value:2.76E-7 ID:SGTC_918|Compound:glabranin|FD-Score:3.46|P-value:2.67E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.48|P-value:2.39E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.72|P-value:9.50E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.62|P-value:1.45E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:4.59|P-value:2.12E-6 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.65|P-value:1.21E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.29|P-value:4.85E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:4.45|P-value:4.10E-6 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.13|P-value:8.65E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:5.68|P-value:6.05E-9 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.15|P-value:7.70E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:-3.31|P-value:4.40E-4 ID:SGTC_1728|Compound:st035614|FD-Score:-4.77|P-value:8.26E-7 ID:SGTC_1806|Compound:st050005|FD-Score:3.12|P-value:8.92E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.09|P-value:2.10E-5 ID:SGTC_1863|Compound:st057641|FD-Score:3.61|P-value:1.49E-4 ID:SGTC_2084|Compound:5306078|FD-Score:3.78|P-value:7.47E-5 ID:SGTC_2116|Compound:5511452|FD-Score:4.60|P-value:2.03E-6 ID:SGTC_2183|Compound:6420625|FD-Score:4.35|P-value:6.42E-6 ID:SGTC_2442|Compound:5158906|FD-Score:3.29|P-value:4.92E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.53|P-value:1.99E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-3.63|P-value:1.32E-4 ID:SGTC_2691|Compound:st077356|FD-Score:4.31|P-value:7.74E-6 ID:SGTC_2694|Compound:st077685|FD-Score:3.76|P-value:8.26E-5 ID:SGTC_2695|Compound:st077686|FD-Score:3.97|P-value:3.47E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-4.78|P-value:7.66E-7 ID:SGTC_2780|Compound:5215298|FD-Score:4.74|P-value:9.99E-7 ID:SGTC_2787|Compound:5284540|FD-Score:-3.20|P-value:6.48E-4 ID:SGTC_2853|Compound:9015042|FD-Score:3.88|P-value:5.12E-5 ID:SGTC_2871|Compound:9038510|FD-Score:-3.11|P-value:8.67E-4 ID:SGTC_2981|Compound:9013952|FD-Score:-3.35|P-value:3.72E-4 ID:SGTC_3024|Compound:9090631|FD-Score:4.71|P-value:1.17E-6 ID:SGTC_3029|Compound:9088683|FD-Score:-3.18|P-value:6.97E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-4.16|P-value:1.41E-5 ID:SGTC_3056|Compound:9094900|FD-Score:3.39|P-value:3.38E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.29|P-value:4.95E-4 ID:SGTC_3158|Compound:9100007|FD-Score:4.00|P-value:3.07E-5 ID:SGTC_3196|Compound:9111891|FD-Score:5.04|P-value:2.17E-7 ID:SGTC_3207|Compound:9114163|FD-Score:-4.36|P-value:5.93E-6 ID:SGTC_3212|Compound:9128307|FD-Score:5.50|P-value:1.72E-8 ID:SGTC_3350|Compound:9150499|FD-Score:3.18|P-value:7.20E-4

Top fitness defect scores for YLR320W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1465 k213-0056 5.68 144.0 µM 6.05E-9 DNA intercalators 4 6.67E-9
2 SGTC_3212 9128307 5.50 49.5 µM 1.72E-8 19 1.89E-8
3 SGTC_3196 9111891 5.04 49.5 µM 2.17E-7 mitochondrial processes 6 2.34E-7
4 SGTC_856 0433-0016 4.99 11.3 µM 2.76E-7 5 2.96E-7
5 SGTC_2780 5215298 4.74 71.4 µM 9.99E-7 20 1.06E-6
6 SGTC_3024 9090631 4.71 49.5 µM 1.17E-6 4 1.24E-6
7 SGTC_177 1327-0068 4.69 8.4 µM 1.30E-6 34 1.39E-6
8 SGTC_580 1123-0028 4.68 41.0 µM 1.38E-6 DNA damage response 15 1.46E-6
9 SGTC_2116 5511452 4.60 16.9 µM 2.03E-6 8 2.15E-6
10 SGTC_1234 0326-0494 4.59 15.5 µM 2.12E-6 9 2.24E-6
11 SGTC_1388 3346-4487 4.45 254.0 µM 4.10E-6 11 4.33E-6
12 SGTC_800 3448-7362 4.41 155.0 µM 4.96E-6 translation 127 5.22E-6
13 SGTC_2183 6420625 4.35 130.5 µM 6.42E-6 mitochondrial processes 40 6.75E-6
14 SGTC_2691 st077356 4.31 60.2 µM 7.74E-6 19 8.14E-6
15 SGTC_1845 st056753 4.09 3.0 µM 2.10E-5 TSC3-RPN4 34 2.19E-5
16 SGTC_3158 9100007 4.00 49.5 µM 3.07E-5 19 3.20E-5
17 SGTC_2695 st077686 3.97 52.0 µM 3.47E-5 34 3.61E-5
18 SGTC_2853 9015042 3.88 58.4 µM 5.12E-5 29 5.32E-5
19 SGTC_2084 5306078 3.78 60.6 µM 7.47E-5 24 7.74E-5
20 SGTC_2694 st077685 3.76 81.9 µM 8.26E-5 endomembrane recycling 37 8.55E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.24E-10 YDL162C_d YDL162C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
0.102 2.79E-9 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.098 1.37E-8 YJL069C UTP18 het Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.096 2.31E-8 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.096 2.66E-8 YEL044W IES6 hom Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
0.096 3.02E-8 YER149C PEA2 hom Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth
0.094 5.48E-8 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.093 7.39E-8 YML057W CMP2 hom Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
0.093 7.95E-8 YCL054W SPB1 het AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
0.093 7.93E-8 YGR247W CPD1 hom Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress
0.092 1.05E-7 YPR137W RRP9 het Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
0.091 1.21E-7 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.091 1.43E-7 YBR143C SUP45 het Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
0.088 3.12E-7 YCL037C SRO9 hom Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
0.086 6.96E-7 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1