YLR322W_d / VPS65

Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR322W / VPS65

Click on Significant Values for Screen Details ID:SGTC_31|Compound:0508-1974|FD-Score:3.83|P-value:2.32E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:5.54|P-value:2.18E-7 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.79|P-value:3.27E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.54|P-value:7.32E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.84|P-value:2.24E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:8.12|P-value:7.20E-14 ID:SGTC_146|Compound:3448-8650|FD-Score:3.42|P-value:8.80E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.70|P-value:4.42E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.90|P-value:1.81E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.67|P-value:1.18E-7 ID:SGTC_306|Compound:1672-3436|FD-Score:4.61|P-value:1.31E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.91|P-value:3.71E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:3.90|P-value:1.83E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.38|P-value:9.89E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.84|P-value:5.01E-6 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.96|P-value:1.80E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:7.63|P-value:1.91E-12 ID:SGTC_625|Compound:k213-0058|FD-Score:3.51|P-value:6.65E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.41|P-value:9.14E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.45|P-value:8.06E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:4.55|P-value:1.66E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.26|P-value:5.74E-9 ID:SGTC_832|Compound:4083-0019|FD-Score:3.47|P-value:7.40E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:3.75|P-value:3.00E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:4.06|P-value:1.06E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.95|P-value:3.18E-6 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.47|P-value:9.16E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:6.17|P-value:9.47E-9 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.60|P-value:4.97E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.69|P-value:3.67E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.71|P-value:3.45E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:5.62|P-value:1.51E-7 ID:SGTC_1327|Compound:1436-0082|FD-Score:3.59|P-value:5.08E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:5.18|P-value:1.16E-6 ID:SGTC_1472|Compound:k841-0093|FD-Score:-3.96|P-value:1.81E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.51|P-value:6.73E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.32|P-value:3.93E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:4.62|P-value:1.22E-5 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:4.04|P-value:1.10E-4 ID:SGTC_1687|Compound:st020778|FD-Score:3.58|P-value:5.23E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-5.21|P-value:1.30E-6 ID:SGTC_1729|Compound:st035615|FD-Score:-3.70|P-value:4.41E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.96|P-value:1.49E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.25|P-value:5.22E-5 ID:SGTC_2002|Compound:4023503|FD-Score:3.44|P-value:8.21E-4 ID:SGTC_2077|Compound:5212015|FD-Score:3.46|P-value:7.74E-4 ID:SGTC_2109|Compound:5483026|FD-Score:4.21|P-value:6.11E-5 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.80|P-value:2.59E-4 ID:SGTC_2356|Compound:9061756|FD-Score:4.19|P-value:6.54E-5 ID:SGTC_2357|Compound:9067248|FD-Score:5.40|P-value:4.30E-7 ID:SGTC_2390|Compound:9097792|FD-Score:3.53|P-value:6.22E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.96|P-value:1.46E-4 ID:SGTC_2462|Compound:5162487|FD-Score:-3.66|P-value:4.98E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-6.06|P-value:2.23E-8 ID:SGTC_2802|Compound:7925815|FD-Score:5.16|P-value:1.25E-6 ID:SGTC_2849|Compound:9019321|FD-Score:4.78|P-value:6.45E-6 ID:SGTC_2881|Compound:9058953|FD-Score:3.85|P-value:2.15E-4 ID:SGTC_2961|Compound:9083290|FD-Score:5.24|P-value:8.92E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.29|P-value:5.54E-5 ID:SGTC_3037|Compound:9091026|FD-Score:3.95|P-value:1.55E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.26|P-value:4.92E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-7.31|P-value:2.03E-11 ID:SGTC_3222|Compound:9128301|FD-Score:3.77|P-value:2.88E-4 ID:SGTC_31|Compound:0508-1974|FD-Score:3.83|P-value:2.32E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:5.54|P-value:2.18E-7 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.79|P-value:3.27E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.54|P-value:7.32E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.84|P-value:2.24E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:8.12|P-value:7.20E-14 ID:SGTC_146|Compound:3448-8650|FD-Score:3.42|P-value:8.80E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.70|P-value:4.42E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.90|P-value:1.81E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.67|P-value:1.18E-7 ID:SGTC_306|Compound:1672-3436|FD-Score:4.61|P-value:1.31E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.91|P-value:3.71E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:3.90|P-value:1.83E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.38|P-value:9.89E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.84|P-value:5.01E-6 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.96|P-value:1.80E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:7.63|P-value:1.91E-12 ID:SGTC_625|Compound:k213-0058|FD-Score:3.51|P-value:6.65E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.41|P-value:9.14E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.45|P-value:8.06E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:4.55|P-value:1.66E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.26|P-value:5.74E-9 ID:SGTC_832|Compound:4083-0019|FD-Score:3.47|P-value:7.40E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:3.75|P-value:3.00E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:4.06|P-value:1.06E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.95|P-value:3.18E-6 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.47|P-value:9.16E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:6.17|P-value:9.47E-9 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.60|P-value:4.97E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.69|P-value:3.67E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.71|P-value:3.45E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:5.62|P-value:1.51E-7 ID:SGTC_1327|Compound:1436-0082|FD-Score:3.59|P-value:5.08E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:5.18|P-value:1.16E-6 ID:SGTC_1472|Compound:k841-0093|FD-Score:-3.96|P-value:1.81E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.51|P-value:6.73E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.32|P-value:3.93E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:4.62|P-value:1.22E-5 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:4.04|P-value:1.10E-4 ID:SGTC_1687|Compound:st020778|FD-Score:3.58|P-value:5.23E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-5.21|P-value:1.30E-6 ID:SGTC_1729|Compound:st035615|FD-Score:-3.70|P-value:4.41E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.96|P-value:1.49E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.25|P-value:5.22E-5 ID:SGTC_2002|Compound:4023503|FD-Score:3.44|P-value:8.21E-4 ID:SGTC_2077|Compound:5212015|FD-Score:3.46|P-value:7.74E-4 ID:SGTC_2109|Compound:5483026|FD-Score:4.21|P-value:6.11E-5 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.80|P-value:2.59E-4 ID:SGTC_2356|Compound:9061756|FD-Score:4.19|P-value:6.54E-5 ID:SGTC_2357|Compound:9067248|FD-Score:5.40|P-value:4.30E-7 ID:SGTC_2390|Compound:9097792|FD-Score:3.53|P-value:6.22E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.96|P-value:1.46E-4 ID:SGTC_2462|Compound:5162487|FD-Score:-3.66|P-value:4.98E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-6.06|P-value:2.23E-8 ID:SGTC_2802|Compound:7925815|FD-Score:5.16|P-value:1.25E-6 ID:SGTC_2849|Compound:9019321|FD-Score:4.78|P-value:6.45E-6 ID:SGTC_2881|Compound:9058953|FD-Score:3.85|P-value:2.15E-4 ID:SGTC_2961|Compound:9083290|FD-Score:5.24|P-value:8.92E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.29|P-value:5.54E-5 ID:SGTC_3037|Compound:9091026|FD-Score:3.95|P-value:1.55E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.26|P-value:4.92E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-7.31|P-value:2.03E-11 ID:SGTC_3222|Compound:9128301|FD-Score:3.77|P-value:2.88E-4

Top fitness defect scores for YLR322W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_121 0557-0495 8.12 61.3 µM 7.20E-14 calcium & mitochondrial duress 9 2.31E-16
2 SGTC_521 r062-0005 7.63 13.8 µM 1.91E-12 1 1.21E-14
3 SGTC_800 3448-7362 6.26 155.0 µM 5.74E-9 translation 40 1.89E-10
4 SGTC_1074 carmofur 6.17 3.8 µM 9.47E-9 exosome 3 3.45E-10
5 SGTC_221 trifluoperazine 5.67 10.3 µM 1.18E-7 NEO1-PIK1 132 7.17E-9
6 SGTC_1306 1185-0270 5.62 9.0 µM 1.51E-7 Golgi 32 9.67E-9
7 SGTC_43 0570-0105 5.54 72.5 µM 2.18E-7 ERG2 45 1.50E-8
8 SGTC_2357 9067248 5.40 200.0 µM 4.30E-7 4 3.40E-8
9 SGTC_2961 9083290 5.24 32.5 µM 8.92E-7 4 8.18E-8
10 SGTC_1453 4237-0541 5.18 195.0 µM 1.16E-6 heme biosynthesis & mitochondrial translocase 10 1.12E-7
11 SGTC_2802 7925815 5.16 54.5 µM 1.25E-6 12 1.23E-7
12 SGTC_951 1171-0579 4.95 82.7 µM 3.18E-6 cell wall 47 3.77E-7
13 SGTC_313 3474-0040 4.91 5.0 µM 3.71E-6 NEO1-PIK1 39 4.53E-7
14 SGTC_514 4466-0038 4.84 2.6 nM 5.01E-6 cell wall 115 6.49E-7
15 SGTC_2849 9019321 4.78 13.0 µM 6.45E-6 19 8.78E-7
16 SGTC_1527 coumarin 4.62 137.0 µM 1.22E-5 8 1.88E-6
17 SGTC_306 1672-3436 4.61 11.6 µM 1.31E-5 Golgi 9 2.06E-6
18 SGTC_727 3032-0951 4.55 202.0 µM 1.66E-5 19 2.74E-6
19 SGTC_1495 4469-0029 4.32 237.0 µM 3.93E-5 95 7.67E-6
20 SGTC_3211 9128781 4.26 49.5 µM 4.92E-5 46 1.00E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.168 1.78E-22 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.161 7.09E-21 YNL246W VPS75 hom NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.143 1.03E-16 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.142 1.48E-16 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.142 1.57E-16 YJL192C SOP4 hom ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER
0.135 4.49E-15 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.135 5.52E-15 YLR120C YPS1 hom Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
0.135 5.62E-15 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.133 1.14E-14 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.132 2.21E-14 YNL295W_p YNL295W_p hom Putative protein of unknown function
0.132 2.38E-14 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.130 4.71E-14 YGL072C_d YGL072C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.127 1.68E-13 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.127 1.64E-13 YKR057W RPS21A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
0.126 3.41E-13 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2