YLR323C / CWC24

Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR323C / CWC24

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:3.61|P-value:3.31E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.28|P-value:1.49E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.00|P-value:4.65E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.58|P-value:2.01E-7 ID:SGTC_209|Compound:4077-0173|FD-Score:-2.81|P-value:7.73E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.95|P-value:5.82E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:-3.22|P-value:1.48E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.51|P-value:3.99E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:2.87|P-value:7.75E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.52|P-value:3.94E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.61|P-value:2.55E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.56|P-value:3.16E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.08|P-value:2.41E-6 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.42|P-value:8.21E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:2.82|P-value:9.54E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-4.55|P-value:1.72E-7 ID:SGTC_1706|Compound:st031321|FD-Score:-3.39|P-value:6.93E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-2.86|P-value:6.46E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.45|P-value:7.14E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.15|P-value:2.54E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.56|P-value:3.23E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-3.00|P-value:3.68E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.13|P-value:2.20E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.92|P-value:6.45E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.44|P-value:7.38E-5 ID:SGTC_2375|Compound:9072683|FD-Score:2.99|P-value:4.92E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.00|P-value:3.73E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.27|P-value:1.53E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-2.90|P-value:5.60E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.11|P-value:2.35E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.88|P-value:8.94E-6 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.89|P-value:7.15E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.15|P-value:2.60E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.76|P-value:7.13E-8 ID:SGTC_2852|Compound:9023854|FD-Score:4.45|P-value:4.25E-7 ID:SGTC_2973|Compound:9094665|FD-Score:2.91|P-value:6.83E-4 ID:SGTC_3113|Compound:9123498|FD-Score:-3.23|P-value:1.43E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.68|P-value:1.80E-5 ID:SGTC_4|Compound:0960-0234|FD-Score:3.61|P-value:3.31E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.28|P-value:1.49E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.00|P-value:4.65E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.58|P-value:2.01E-7 ID:SGTC_209|Compound:4077-0173|FD-Score:-2.81|P-value:7.73E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.95|P-value:5.82E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:-3.22|P-value:1.48E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.51|P-value:3.99E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:2.87|P-value:7.75E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.52|P-value:3.94E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.61|P-value:2.55E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.56|P-value:3.16E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.08|P-value:2.41E-6 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.42|P-value:8.21E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:2.82|P-value:9.54E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-4.55|P-value:1.72E-7 ID:SGTC_1706|Compound:st031321|FD-Score:-3.39|P-value:6.93E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-2.86|P-value:6.46E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.45|P-value:7.14E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.15|P-value:2.54E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.56|P-value:3.23E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-3.00|P-value:3.68E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.13|P-value:2.20E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.92|P-value:6.45E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.44|P-value:7.38E-5 ID:SGTC_2375|Compound:9072683|FD-Score:2.99|P-value:4.92E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.00|P-value:3.73E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.27|P-value:1.53E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-2.90|P-value:5.60E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.11|P-value:2.35E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.88|P-value:8.94E-6 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.89|P-value:7.15E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.15|P-value:2.60E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.76|P-value:7.13E-8 ID:SGTC_2852|Compound:9023854|FD-Score:4.45|P-value:4.25E-7 ID:SGTC_2973|Compound:9094665|FD-Score:2.91|P-value:6.83E-4 ID:SGTC_3113|Compound:9123498|FD-Score:-3.23|P-value:1.43E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.68|P-value:1.80E-5

Top fitness defect scores for YLR323C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2814 7977482 4.76 43.0 µM 7.13E-8 7 9.79E-7 0.16
2 SGTC_124 0136-0217 4.58 7.1 µM 2.01E-7 mitochondrial stress 17 2.28E-6 0.08
3 SGTC_2852 9023854 4.45 52.0 µM 4.25E-7 4 4.22E-6 0.08
4 SGTC_2638 epoxomicin 3.88 18.0 µM 8.94E-6 4 5.13E-5 0.46
5 SGTC_4 0960-0234 3.61 25.7 µM 3.31E-5 Golgi 15 1.51E-4 0.32
6 SGTC_1783 st039459 3.45 87.5 µM 7.14E-5 Golgi 11 2.83E-4 0.03
7 SGTC_2363 9053361 3.44 176.0 µM 7.38E-5 Golgi 16 2.91E-4 0.02
8 SGTC_1179 1021-1431 3.42 9.3 µM 8.21E-5 10 3.18E-4 0.04
9 SGTC_41 0478-0632 3.28 82.6 µM 1.49E-4 18 5.19E-4 0.00
10 SGTC_2439 5492030 3.27 7.2 µM 1.53E-4 13 5.31E-4 0.09
11 SGTC_1895 st058434 3.15 18.3 µM 2.54E-4 9 8.07E-4 0.01
12 SGTC_2796 7949634 3.15 45.5 µM 2.60E-4 17 8.23E-4 0.07
13 SGTC_45 0kpi-0036 3.00 14.9 µM 4.65E-4 excess fatty acid 14 0.00133 0.07
14 SGTC_2375 9072683 2.99 200.0 µM 4.92E-4 mitochondrial processes 12 0.00139 0.08
15 SGTC_280 3448-3607 2.95 3.0 µM 5.82E-4 60S ribosome export 9 0.00160 0.15
16 SGTC_2115 tiliquinol 2.92 12.8 µM 6.45E-4 14 0.00174 0.05
17 SGTC_2973 9094665 2.91 18.0 µM 6.83E-4 NEO1 35 0.00183 0.02
18 SGTC_2640 pentamidine 2.89 10.4 µM 7.15E-4 mitochondrial processes 31 0.00190 0.02
19 SGTC_883 0850-0245 2.87 24.6 µM 7.75E-4 cell wall 27 0.00203 0.06
20 SGTC_1248 0469-0687 2.82 58.6 µM 9.54E-4 22 0.00241 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.269 7.99E-57 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.223 5.69E-39 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.204 6.41E-33 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.196 2.12E-30 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.188 4.24E-28 YLR259C HSP60 het Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
0.165 6.93E-22 YGR127W_p YGR127W_p hom Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
0.162 2.89E-21 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.153 6.19E-19 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.152 8.45E-19 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.152 9.38E-19 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.150 1.98E-18 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.146 1.51E-17 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.144 5.29E-17 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.142 1.21E-16 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.141 1.82E-16 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants