YLR328W / NMA1

Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR328W / NMA1

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:-3.83|P-value:2.45E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-4.11|P-value:9.16E-5 ID:SGTC_499|Compound:genistein|FD-Score:3.75|P-value:4.01E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.89|P-value:2.45E-4 ID:SGTC_509|Compound:pifithrin|FD-Score:3.61|P-value:6.11E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:4.41|P-value:3.94E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:4.60|P-value:1.87E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:5.09|P-value:2.51E-6 ID:SGTC_1805|Compound:st050013|FD-Score:-4.42|P-value:2.86E-5 ID:SGTC_1819|Compound:st053448|FD-Score:4.67|P-value:1.39E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.99|P-value:1.75E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.46|P-value:8.03E-4 ID:SGTC_2045|Compound:5106652|FD-Score:-3.44|P-value:8.53E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-4.77|P-value:7.02E-6 ID:SGTC_2435|Compound:5706476|FD-Score:-3.71|P-value:3.59E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:-4.24|P-value:5.70E-5 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.98|P-value:1.43E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.50|P-value:2.14E-5 ID:SGTC_3226|Compound:9131256|FD-Score:-3.70|P-value:3.71E-4 ID:SGTC_3230|Compound:9131665|FD-Score:-4.70|P-value:9.53E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.83|P-value:2.45E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-4.11|P-value:9.16E-5 ID:SGTC_499|Compound:genistein|FD-Score:3.75|P-value:4.01E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.89|P-value:2.45E-4 ID:SGTC_509|Compound:pifithrin|FD-Score:3.61|P-value:6.11E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:4.41|P-value:3.94E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:4.60|P-value:1.87E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:5.09|P-value:2.51E-6 ID:SGTC_1805|Compound:st050013|FD-Score:-4.42|P-value:2.86E-5 ID:SGTC_1819|Compound:st053448|FD-Score:4.67|P-value:1.39E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.99|P-value:1.75E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.46|P-value:8.03E-4 ID:SGTC_2045|Compound:5106652|FD-Score:-3.44|P-value:8.53E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-4.77|P-value:7.02E-6 ID:SGTC_2435|Compound:5706476|FD-Score:-3.71|P-value:3.59E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:-4.24|P-value:5.70E-5 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.98|P-value:1.43E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.50|P-value:2.14E-5 ID:SGTC_3226|Compound:9131256|FD-Score:-3.70|P-value:3.71E-4 ID:SGTC_3230|Compound:9131665|FD-Score:-4.70|P-value:9.53E-6

Top fitness defect scores for YLR328W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1568 digitonin 5.09 820.0 nM 2.51E-6 sphingolipid biosynthesis & PDR1 16 1.81E-7
2 SGTC_1819 st053448 4.67 58.6 µM 1.39E-5 amide catabolism 7 1.48E-6
3 SGTC_880 0833-0487 4.60 87.2 µM 1.87E-5 mitochondrial processes 9 2.12E-6
4 SGTC_638 3970-0837 4.41 4.1 µM 3.94E-5 25 5.27E-6
5 SGTC_1821 st053465 3.99 65.6 µM 1.75E-4 4 3.27E-5
6 SGTC_508 roscovitine 3.89 141.0 µM 2.45E-4 7 4.93E-5
7 SGTC_499 genistein 3.75 192.0 µM 4.01E-4 3 8.99E-5
8 SGTC_509 pifithrin 3.61 175.0 µM 6.11E-4 mitochondrial processes 8 1.50E-4
9 SGTC_2427 5545055 3.38 42.8 µM 0.00126 34 3.65E-4
10 SGTC_814 3342-0569 3.33 96.0 µM 0.00144 42 4.27E-4
11 SGTC_2855 9016525 3.22 58.4 µM 0.00199 48 6.36E-4
12 SGTC_1822 st052468 3.20 42.0 µM 0.00215 28 6.97E-4
13 SGTC_1016 4226-1401 3.19 38.0 µM 0.00219 RNA pol III & RNase P/MRP 43 7.13E-4
14 SGTC_632 0781-2353 3.18 4.7 µM 0.00227 Golgi 80 7.47E-4
15 SGTC_2293 7624732 3.15 200.0 µM 0.00247 53 8.26E-4
16 SGTC_1310 1188-0297 3.09 994.0 nM 0.00286 azole & statin 69 9.86E-4
17 SGTC_785 1270-0111 3.09 65.3 µM 0.00287 64 9.91E-4
18 SGTC_2429 5902844 3.08 64.4 µM 0.00301 72 0.00105
19 SGTC_2039 Aminomethylbenzodioxan 3.07 200.0 µM 0.00303 26 0.00106
20 SGTC_3191 9110352 3.03 49.5 µM 0.00343 40 0.00123

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.220 4.75E-38 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.131 2.70E-14 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.129 6.75E-14 YPL041C_p YPL041C_p hom Protein of unknown function involved in maintenance of proper telomere length
0.124 5.19E-13 YGR096W TPC1 hom Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
0.119 4.38E-12 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.102 2.92E-9 YPR079W MRL1 hom Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
0.101 3.90E-9 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.100 6.10E-9 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.098 1.16E-8 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.096 2.19E-8 YKR071C DRE2 het Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
0.095 3.17E-8 YBR176W ECM31 hom Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
0.095 3.29E-8 YNR032W PPG1 hom Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
0.092 9.06E-8 YJR068W RFC2 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.092 1.02E-7 YDL235C YPD1 het Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
0.091 1.32E-7 YMR031C EIS1 hom Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress