YLR332W / MID2

O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR332W / MID2

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.52|P-value:2.63E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.36|P-value:8.32E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:11.40|P-value:6.00E-30 ID:SGTC_71|Compound:0417-1750|FD-Score:5.37|P-value:5.19E-8 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.30|P-value:3.95E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:11.50|P-value:1.84E-30 ID:SGTC_160|Compound:3453-0671|FD-Score:3.32|P-value:5.40E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:4.23|P-value:1.46E-5 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.39|P-value:7.07E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.12|P-value:6.38E-10 ID:SGTC_299|Compound:1273-0059|FD-Score:5.22|P-value:1.20E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:6.29|P-value:2.24E-10 ID:SGTC_307|Compound:1501-1223|FD-Score:-3.53|P-value:1.72E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.04|P-value:3.09E-7 ID:SGTC_354|Compound:2783-3500|FD-Score:8.61|P-value:5.69E-18 ID:SGTC_360|Compound:0957-0399|FD-Score:5.50|P-value:2.58E-8 ID:SGTC_422|Compound:cantharidin|FD-Score:8.30|P-value:8.24E-17 ID:SGTC_495|Compound:decylubiquinone|FD-Score:4.02|P-value:3.65E-5 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.43|P-value:3.67E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.58|P-value:2.12E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.40|P-value:4.02E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:8.02|P-value:7.63E-16 ID:SGTC_650|Compound:1328-0195|FD-Score:-3.58|P-value:1.40E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:12.10|P-value:1.48E-33 ID:SGTC_666|Compound:0272-0154|FD-Score:21.70|P-value:1.08E-104 ID:SGTC_670|Compound:4204-0025|FD-Score:3.28|P-value:6.17E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:3.36|P-value:4.74E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:3.92|P-value:5.38E-5 ID:SGTC_761|Compound:k216-0224|FD-Score:4.94|P-value:5.11E-7 ID:SGTC_814|Compound:3342-0569|FD-Score:4.14|P-value:2.20E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.29|P-value:1.10E-5 ID:SGTC_918|Compound:glabranin|FD-Score:4.05|P-value:3.21E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:4.00|P-value:3.95E-5 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.28|P-value:6.14E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.70|P-value:1.33E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:-3.66|P-value:1.04E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:3.49|P-value:2.95E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.89|P-value:6.12E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-5.11|P-value:1.22E-7 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.22|P-value:1.18E-7 ID:SGTC_1178|Compound:0988-0033|FD-Score:5.50|P-value:2.50E-8 ID:SGTC_1181|Compound:1222-0449|FD-Score:5.20|P-value:1.27E-7 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.87|P-value:6.76E-5 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:3.52|P-value:2.57E-4 ID:SGTC_1632|Compound:st005650|FD-Score:3.55|P-value:2.32E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.03|P-value:3.46E-5 ID:SGTC_1727|Compound:st034309|FD-Score:-3.30|P-value:3.96E-4 ID:SGTC_1804|Compound:st050076|FD-Score:4.98|P-value:4.11E-7 ID:SGTC_1808|Compound:st050063|FD-Score:3.47|P-value:3.20E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.37|P-value:4.54E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-3.26|P-value:4.64E-4 ID:SGTC_1990|Compound:st072050|FD-Score:3.32|P-value:5.50E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.20|P-value:8.21E-4 ID:SGTC_2004|Compound:4023728|FD-Score:-3.42|P-value:2.60E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.89|P-value:6.29E-5 ID:SGTC_2007|Compound:4011539|FD-Score:16.10|P-value:5.26E-58 ID:SGTC_2009|Compound:4012297|FD-Score:14.70|P-value:5.78E-49 ID:SGTC_2272|Compound:7805186|FD-Score:5.49|P-value:2.68E-8 ID:SGTC_2341|Compound:7987486|FD-Score:3.52|P-value:2.57E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.35|P-value:4.81E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.28|P-value:6.32E-4 ID:SGTC_2399|Compound:5884133|FD-Score:9.95|P-value:1.99E-23 ID:SGTC_2424|Compound:5537928|FD-Score:3.29|P-value:6.04E-4 ID:SGTC_2485|Compound:5633444|FD-Score:4.93|P-value:5.34E-7 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.08|P-value:2.86E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.35|P-value:8.45E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.91|P-value:5.74E-5 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-3.38|P-value:2.97E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.86|P-value:7.07E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.37|P-value:4.59E-4 ID:SGTC_2781|Compound:5567276|FD-Score:3.58|P-value:2.06E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.22|P-value:5.23E-4 ID:SGTC_2868|Compound:9035608|FD-Score:3.15|P-value:9.64E-4 ID:SGTC_2912|Compound:7964298|FD-Score:10.20|P-value:1.77E-24 ID:SGTC_2950|Compound:9072895|FD-Score:4.51|P-value:4.08E-6 ID:SGTC_2952|Compound:9073731|FD-Score:4.00|P-value:3.86E-5 ID:SGTC_3253|Compound:9135987|FD-Score:3.63|P-value:1.72E-4 ID:SGTC_3266|Compound:9136860|FD-Score:4.96|P-value:4.66E-7 ID:SGTC_3311|Compound:9131146|FD-Score:4.19|P-value:1.74E-5 ID:SGTC_3321|Compound:9137793|FD-Score:6.83|P-value:5.86E-12 ID:SGTC_3350|Compound:9150499|FD-Score:3.15|P-value:9.57E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:3.52|P-value:2.63E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.36|P-value:8.32E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:11.40|P-value:6.00E-30 ID:SGTC_71|Compound:0417-1750|FD-Score:5.37|P-value:5.19E-8 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.30|P-value:3.95E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:11.50|P-value:1.84E-30 ID:SGTC_160|Compound:3453-0671|FD-Score:3.32|P-value:5.40E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:4.23|P-value:1.46E-5 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.39|P-value:7.07E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.12|P-value:6.38E-10 ID:SGTC_299|Compound:1273-0059|FD-Score:5.22|P-value:1.20E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:6.29|P-value:2.24E-10 ID:SGTC_307|Compound:1501-1223|FD-Score:-3.53|P-value:1.72E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.04|P-value:3.09E-7 ID:SGTC_354|Compound:2783-3500|FD-Score:8.61|P-value:5.69E-18 ID:SGTC_360|Compound:0957-0399|FD-Score:5.50|P-value:2.58E-8 ID:SGTC_422|Compound:cantharidin|FD-Score:8.30|P-value:8.24E-17 ID:SGTC_495|Compound:decylubiquinone|FD-Score:4.02|P-value:3.65E-5 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.43|P-value:3.67E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.58|P-value:2.12E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.40|P-value:4.02E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:8.02|P-value:7.63E-16 ID:SGTC_650|Compound:1328-0195|FD-Score:-3.58|P-value:1.40E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:12.10|P-value:1.48E-33 ID:SGTC_666|Compound:0272-0154|FD-Score:21.70|P-value:1.08E-104 ID:SGTC_670|Compound:4204-0025|FD-Score:3.28|P-value:6.17E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:3.36|P-value:4.74E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:3.92|P-value:5.38E-5 ID:SGTC_761|Compound:k216-0224|FD-Score:4.94|P-value:5.11E-7 ID:SGTC_814|Compound:3342-0569|FD-Score:4.14|P-value:2.20E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.29|P-value:1.10E-5 ID:SGTC_918|Compound:glabranin|FD-Score:4.05|P-value:3.21E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:4.00|P-value:3.95E-5 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.28|P-value:6.14E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.70|P-value:1.33E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:-3.66|P-value:1.04E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:3.49|P-value:2.95E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.89|P-value:6.12E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-5.11|P-value:1.22E-7 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.22|P-value:1.18E-7 ID:SGTC_1178|Compound:0988-0033|FD-Score:5.50|P-value:2.50E-8 ID:SGTC_1181|Compound:1222-0449|FD-Score:5.20|P-value:1.27E-7 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.87|P-value:6.76E-5 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:3.52|P-value:2.57E-4 ID:SGTC_1632|Compound:st005650|FD-Score:3.55|P-value:2.32E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.03|P-value:3.46E-5 ID:SGTC_1727|Compound:st034309|FD-Score:-3.30|P-value:3.96E-4 ID:SGTC_1804|Compound:st050076|FD-Score:4.98|P-value:4.11E-7 ID:SGTC_1808|Compound:st050063|FD-Score:3.47|P-value:3.20E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.37|P-value:4.54E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-3.26|P-value:4.64E-4 ID:SGTC_1990|Compound:st072050|FD-Score:3.32|P-value:5.50E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.20|P-value:8.21E-4 ID:SGTC_2004|Compound:4023728|FD-Score:-3.42|P-value:2.60E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.89|P-value:6.29E-5 ID:SGTC_2007|Compound:4011539|FD-Score:16.10|P-value:5.26E-58 ID:SGTC_2009|Compound:4012297|FD-Score:14.70|P-value:5.78E-49 ID:SGTC_2272|Compound:7805186|FD-Score:5.49|P-value:2.68E-8 ID:SGTC_2341|Compound:7987486|FD-Score:3.52|P-value:2.57E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.35|P-value:4.81E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.28|P-value:6.32E-4 ID:SGTC_2399|Compound:5884133|FD-Score:9.95|P-value:1.99E-23 ID:SGTC_2424|Compound:5537928|FD-Score:3.29|P-value:6.04E-4 ID:SGTC_2485|Compound:5633444|FD-Score:4.93|P-value:5.34E-7 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.08|P-value:2.86E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.35|P-value:8.45E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.91|P-value:5.74E-5 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-3.38|P-value:2.97E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.86|P-value:7.07E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.37|P-value:4.59E-4 ID:SGTC_2781|Compound:5567276|FD-Score:3.58|P-value:2.06E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.22|P-value:5.23E-4 ID:SGTC_2868|Compound:9035608|FD-Score:3.15|P-value:9.64E-4 ID:SGTC_2912|Compound:7964298|FD-Score:10.20|P-value:1.77E-24 ID:SGTC_2950|Compound:9072895|FD-Score:4.51|P-value:4.08E-6 ID:SGTC_2952|Compound:9073731|FD-Score:4.00|P-value:3.86E-5 ID:SGTC_3253|Compound:9135987|FD-Score:3.63|P-value:1.72E-4 ID:SGTC_3266|Compound:9136860|FD-Score:4.96|P-value:4.66E-7 ID:SGTC_3311|Compound:9131146|FD-Score:4.19|P-value:1.74E-5 ID:SGTC_3321|Compound:9137793|FD-Score:6.83|P-value:5.86E-12 ID:SGTC_3350|Compound:9150499|FD-Score:3.15|P-value:9.57E-4

Top fitness defect scores for YLR332W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_666 0272-0154 21.70 136.0 µM 1.08E-104 cell wall 1 3.99E-105
2 SGTC_2007 4011539 16.10 71.8 µM 5.26E-58 cell wall signaling 5 2.45E-58
3 SGTC_2009 4012297 14.70 12.1 µM 5.78E-49 cell wall signaling 5 2.85E-49
4 SGTC_663 1486-0733 12.10 13.9 µM 1.48E-33 cell wall signaling 8 8.17E-34
5 SGTC_138 0850-0259 11.50 40.9 µM 1.84E-30 cell wall 32 1.04E-30
6 SGTC_58 0957-0393 11.40 9.0 µM 6.00E-30 cell wall 58 3.43E-30
7 SGTC_2912 7964298 10.20 10.9 µM 1.77E-24 cell wall 14 1.07E-24
8 SGTC_2399 5884133 9.95 200.0 µM 1.99E-23 cell wall signaling 13 1.21E-23
9 SGTC_354 2783-3500 8.61 4.9 µM 5.69E-18 cell wall 9 3.69E-18
10 SGTC_422 cantharidin 8.30 155.0 µM 8.24E-17 cell wall signaling 9 5.42E-17
11 SGTC_586 0957-0265 8.02 8.2 µM 7.63E-16 cell wall 7 5.08E-16
12 SGTC_3321 9137793 6.83 26.8 µM 5.86E-12 cell wall 26 4.13E-12
13 SGTC_304 1310-0298 6.29 97.0 µM 2.24E-10 cell wall 16 1.62E-10
14 SGTC_255 tunicamycin 6.12 200.5 nM 6.38E-10 unfolded protein response 32 4.65E-10
15 SGTC_1178 0988-0033 5.50 191.0 µM 2.50E-8 7 1.87E-8
16 SGTC_360 0957-0399 5.50 1.0 µM 2.58E-8 cell wall 17 1.94E-8
17 SGTC_2272 7805186 5.49 180.0 µM 2.68E-8 cell wall signaling 9 2.01E-8
18 SGTC_71 0417-1750 5.37 47.8 µM 5.19E-8 Golgi 22 3.92E-8
19 SGTC_1133 3473-0247 5.22 50.0 µM 1.18E-7 RSC complex & mRNA processing 19 9.01E-8
20 SGTC_299 1273-0059 5.22 1.4 µM 1.20E-7 9 9.16E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.697 0 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.635 0 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.459 4.14E-174 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.443 1.99E-161 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.398 1.88E-127 YJL095W BCK1 hom Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.359 2.05E-102 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.358 7.12E-102 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.347 4.45E-95 YHR030C SLT2 hom Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.346 3.28E-95 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.345 1.77E-94 YOR008C SLG1 hom Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.342 1.11E-92 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.320 1.11E-80 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.318 1.08E-79 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.298 7.91E-70 YPL089C RLM1 hom MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
0.294 4.31E-68 YNL233W BNI4 hom Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p