HIPHOP chemogenomics database

YLR353W / BUD8

Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR353W / BUD8

Click on Significant Values for Screen Details

Top fitness defect scores for YLR353W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2309	7664088	6.02	163.9 µM	3.13E-12		5	8.86E-10
2	SGTC_2292	7671323	5.12	168.1 µM	2.32E-9	RNA processing & uracil transport	15	1.50E-7
3	SGTC_2310	7668677	4.95	200.0 µM	7.25E-9		14	3.62E-7
4	SGTC_998	3996-0085	4.75	177.0 µM	2.69E-8		8	1.00E-6
5	SGTC_2103	5378138	4.07	10.0 µM	1.57E-6		13	2.37E-5
6	SGTC_2097	5376186	3.93	200.0 µM	3.32E-6		20	4.24E-5
7	SGTC_2337	9009472	3.72	200.0 µM	9.80E-6		40	9.86E-5
8	SGTC_996	3970-0799	3.26	1.8 µM	8.94E-5		135	5.52E-4
9	SGTC_3039	9090270	3.26	49.5 µM	9.08E-5	RPP1 & pyrimidine depletion	26	5.59E-4
10	SGTC_3323	9138564	3.10	53.9 µM	1.81E-4		53	9.59E-4
11	SGTC_2757	clomiphene	3.09	4.2 µM	1.92E-4	fatty acid desaturase (OLE1)	86	0.00100
12	SGTC_699	0375-0592	3.08	111.0 µM	1.97E-4	iron homeostasis	57	0.00102
13	SGTC_2093	5331342	3.05	194.4 µM	2.29E-4		53	0.00115
14	SGTC_1808	st050063	3.00	61.5 µM	2.85E-4		48	0.00137
15	SGTC_1561	5-methoxyflavone	2.97	52.9 µM	3.14E-4		23	0.00147
16	SGTC_705	1124-0212	2.90	310.0 µM	4.22E-4		61	0.00185
17	SGTC_1527	coumarin	2.90	137.0 µM	4.32E-4		66	0.00189
18	SGTC_2584	androsterone	2.84	48.7 µM	5.45E-4	cell wall	230	0.00227
19	SGTC_189	3970-0742	2.83	2.1 µM	5.68E-4		35	0.00234
20	SGTC_2374	9071832	2.77	200.0 µM	7.05E-4	endomembrane recycling	49	0.00277

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.322	6.21E-82	YKL131W_d	YKL131W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.282	1.84E-62	YFL063W_d	YFL063W_d	hom	Dubious open reading frame, based on available experimental and comparative sequence data
0.275	4.14E-59	YKL220C	FRE2	hom	Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.256	1.54E-51	YOR037W	CYC2	hom	Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.255	4.67E-51	YKR023W_p	YKR023W_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.243	4.61E-46	YDL110C	TMA17	hom	Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.239	8.39E-45	YOR298C-A	MBF1	hom	Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.236	9.44E-44	YBR262C	AIM5	hom	Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.234	7.51E-43	YBR207W	FTH1	hom	Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.228	6.79E-41	YKL179C	COY1	hom	Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.226	2.89E-40	YBL015W	ACH1	hom	Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.223	3.20E-39	YPR010C	RPA135	het	RNA polymerase I second largest subunit A135
0.223	5.37E-39	YBL036C	YBL036C	hom	Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.219	1.22E-37	YAL060W	BDH1	hom	NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
0.218	2.56E-37	YMR139W	RIM11	hom	Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress