YLR355C / ILV5

Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR355C / ILV5

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:4.36|P-value:1.33E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:3.84|P-value:6.60E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:8.43|P-value:8.78E-13 ID:SGTC_90|Compound:0870-0863|FD-Score:4.13|P-value:2.79E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:5.07|P-value:1.14E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:4.21|P-value:2.19E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:3.79|P-value:7.64E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.52|P-value:7.98E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:4.09|P-value:3.10E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:5.44|P-value:2.71E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:6.07|P-value:1.94E-7 ID:SGTC_177|Compound:1327-0068|FD-Score:4.34|P-value:1.44E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.87|P-value:6.02E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.11|P-value:9.74E-6 ID:SGTC_355|Compound:1311-2252|FD-Score:3.96|P-value:4.60E-4 ID:SGTC_459|Compound:nigericin|FD-Score:5.12|P-value:9.38E-6 ID:SGTC_674|Compound:0498-0004|FD-Score:4.19|P-value:2.31E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.94|P-value:4.96E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:5.69|P-value:9.62E-7 ID:SGTC_966|Compound:1300-0360|FD-Score:8.68|P-value:1.95E-13 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.76|P-value:8.24E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.55|P-value:7.22E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.75|P-value:3.56E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.02|P-value:3.79E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:4.93|P-value:1.84E-5 ID:SGTC_1772|Compound:st048439|FD-Score:4.20|P-value:2.25E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.93|P-value:5.07E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.55|P-value:7.08E-5 ID:SGTC_1793|Compound:st048256|FD-Score:4.93|P-value:1.84E-5 ID:SGTC_1797|Compound:st048695|FD-Score:7.24|P-value:7.01E-10 ID:SGTC_1831|Compound:st055328|FD-Score:4.66|P-value:4.94E-5 ID:SGTC_1919|Compound:st067113|FD-Score:-5.18|P-value:7.06E-6 ID:SGTC_2055|Compound:5235184|FD-Score:4.50|P-value:8.53E-5 ID:SGTC_2183|Compound:6420625|FD-Score:3.88|P-value:5.82E-4 ID:SGTC_2225|Compound:6654152|FD-Score:4.46|P-value:9.48E-5 ID:SGTC_2233|Compound:sulbentine|FD-Score:8.70|P-value:1.70E-13 ID:SGTC_2612|Compound:dictamnine|FD-Score:4.01|P-value:4.02E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.21|P-value:2.17E-4 ID:SGTC_2696|Compound:st077691|FD-Score:5.01|P-value:1.37E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:-4.04|P-value:3.55E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-13.50|P-value:7.73E-30 ID:SGTC_2754|Compound:granisetron|FD-Score:-17.90|P-value:7.30E-51 ID:SGTC_2759|Compound:disulfiram|FD-Score:8.31|P-value:1.84E-12 ID:SGTC_2760|Compound:etidronate|FD-Score:6.75|P-value:8.38E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.74|P-value:8.76E-4 ID:SGTC_2799|Compound:7812892|FD-Score:4.95|P-value:1.72E-5 ID:SGTC_2805|Compound:7950783|FD-Score:4.28|P-value:1.72E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.08|P-value:3.26E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.75|P-value:8.66E-4 ID:SGTC_2980|Compound:9014342|FD-Score:4.46|P-value:9.67E-5 ID:SGTC_3045|Compound:9092950|FD-Score:-4.12|P-value:2.78E-4 ID:SGTC_3077|Compound:9117319|FD-Score:5.28|P-value:5.06E-6 ID:SGTC_3208|Compound:9114115|FD-Score:4.03|P-value:3.71E-4 ID:SGTC_3281|Compound:9140613|FD-Score:-4.26|P-value:1.80E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:4.36|P-value:1.33E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:3.84|P-value:6.60E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:8.43|P-value:8.78E-13 ID:SGTC_90|Compound:0870-0863|FD-Score:4.13|P-value:2.79E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:5.07|P-value:1.14E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:4.21|P-value:2.19E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:3.79|P-value:7.64E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.52|P-value:7.98E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:4.09|P-value:3.10E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:5.44|P-value:2.71E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:6.07|P-value:1.94E-7 ID:SGTC_177|Compound:1327-0068|FD-Score:4.34|P-value:1.44E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.87|P-value:6.02E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.11|P-value:9.74E-6 ID:SGTC_355|Compound:1311-2252|FD-Score:3.96|P-value:4.60E-4 ID:SGTC_459|Compound:nigericin|FD-Score:5.12|P-value:9.38E-6 ID:SGTC_674|Compound:0498-0004|FD-Score:4.19|P-value:2.31E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.94|P-value:4.96E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:5.69|P-value:9.62E-7 ID:SGTC_966|Compound:1300-0360|FD-Score:8.68|P-value:1.95E-13 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.76|P-value:8.24E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.55|P-value:7.22E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.75|P-value:3.56E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.02|P-value:3.79E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:4.93|P-value:1.84E-5 ID:SGTC_1772|Compound:st048439|FD-Score:4.20|P-value:2.25E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.93|P-value:5.07E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.55|P-value:7.08E-5 ID:SGTC_1793|Compound:st048256|FD-Score:4.93|P-value:1.84E-5 ID:SGTC_1797|Compound:st048695|FD-Score:7.24|P-value:7.01E-10 ID:SGTC_1831|Compound:st055328|FD-Score:4.66|P-value:4.94E-5 ID:SGTC_1919|Compound:st067113|FD-Score:-5.18|P-value:7.06E-6 ID:SGTC_2055|Compound:5235184|FD-Score:4.50|P-value:8.53E-5 ID:SGTC_2183|Compound:6420625|FD-Score:3.88|P-value:5.82E-4 ID:SGTC_2225|Compound:6654152|FD-Score:4.46|P-value:9.48E-5 ID:SGTC_2233|Compound:sulbentine|FD-Score:8.70|P-value:1.70E-13 ID:SGTC_2612|Compound:dictamnine|FD-Score:4.01|P-value:4.02E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.21|P-value:2.17E-4 ID:SGTC_2696|Compound:st077691|FD-Score:5.01|P-value:1.37E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:-4.04|P-value:3.55E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-13.50|P-value:7.73E-30 ID:SGTC_2754|Compound:granisetron|FD-Score:-17.90|P-value:7.30E-51 ID:SGTC_2759|Compound:disulfiram|FD-Score:8.31|P-value:1.84E-12 ID:SGTC_2760|Compound:etidronate|FD-Score:6.75|P-value:8.38E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.74|P-value:8.76E-4 ID:SGTC_2799|Compound:7812892|FD-Score:4.95|P-value:1.72E-5 ID:SGTC_2805|Compound:7950783|FD-Score:4.28|P-value:1.72E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.08|P-value:3.26E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.75|P-value:8.66E-4 ID:SGTC_2980|Compound:9014342|FD-Score:4.46|P-value:9.67E-5 ID:SGTC_3045|Compound:9092950|FD-Score:-4.12|P-value:2.78E-4 ID:SGTC_3077|Compound:9117319|FD-Score:5.28|P-value:5.06E-6 ID:SGTC_3208|Compound:9114115|FD-Score:4.03|P-value:3.71E-4 ID:SGTC_3281|Compound:9140613|FD-Score:-4.26|P-value:1.80E-4

Top fitness defect scores for YLR355C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2233 sulbentine 8.70 40.4 µM 1.70E-13 12 1.66E-18 0.24
2 SGTC_966 1300-0360 8.68 30.2 µM 1.95E-13 heme requiring 4 2.01E-18 2.11
3 SGTC_70 0141-0289 8.43 43.3 µM 8.78E-13 mitochondrial stress 4 1.71E-17 0.78
4 SGTC_2759 disulfiram 8.31 3.4 µM 1.84E-12 3 4.89E-17 0.65
5 SGTC_1797 st048695 7.24 56.8 µM 7.01E-10 NEO1 3 2.26E-13 0.01
6 SGTC_2760 etidronate 6.75 98.0 µM 8.38E-9 1 7.59E-12 2.05
7 SGTC_158 k038-0087 6.07 26.4 µM 1.94E-7 ubiquinone biosynthesis & proteasome 14 6.49E-10 0.47
8 SGTC_730 1436-0052 5.69 41.2 µM 9.62E-7 mitochondrial processes 6 6.24E-9 0.09
9 SGTC_153 4077-0274 5.44 519.7 µM 2.71E-6 3 2.70E-8 0.83
10 SGTC_3077 9117319 5.28 49.5 µM 5.06E-6 3 6.50E-8 0.56
11 SGTC_459 nigericin 5.12 15.3 µM 9.38E-6 4 1.55E-7 0.28
12 SGTC_275 trichlorophene 5.11 5.7 µM 9.74E-6 mitochondrial stress 2 1.64E-7 0.66
13 SGTC_120 rhodamine 6G 5.07 52.3 µM 1.14E-5 mitochondrial processes 9 2.04E-7 0.37
14 SGTC_2696 st077691 5.01 74.3 µM 1.37E-5 8 2.66E-7 0.53
15 SGTC_2799 7812892 4.95 81.8 µM 1.72E-5 superoxide 10 3.65E-7 0.24
16 SGTC_1793 st048256 4.93 72.2 µM 1.84E-5 2 4.01E-7 0.23
17 SGTC_1533 dihydroergotamine mesylate 4.93 29.4 µM 1.84E-5 DNA intercalators 3 4.02E-7 0.40
18 SGTC_1371 2421-0008 4.75 32.7 µM 3.56E-5 mitochondrial processes 16 1.02E-6 0.02
19 SGTC_1831 st055328 4.66 12.0 µM 4.94E-5 heme biosynthesis & mitochondrial translocase 9 1.61E-6 0.22
20 SGTC_1785 bathophenanthroline 4.55 24.0 µM 7.08E-5 heme requiring 17 2.67E-6 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.280 2.76E-61 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.252 1.20E-49 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.190 1.32E-28 YPL266W DIM1 het Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.184 6.93E-27 YBL105C PKC1 het Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
0.173 7.51E-24 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.164 1.20E-21 YJL031C BET4 het Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.151 1.48E-18 YDR016C DAD1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.145 3.90E-17 YEL034W HYP2 het Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.144 5.84E-17 YCR024C SLM5 hom Mitochondrial asparaginyl-tRNA synthetase
0.137 1.46E-15 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.135 3.37E-15 YPR166C MRP2 hom Mitochondrial ribosomal protein of the small subunit
0.134 5.69E-15 YFR037C RSC8 het Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.131 2.85E-14 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.130 3.50E-14 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.127 1.45E-13 YGL068W MNP1 het Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth