YLR359W / ADE13

Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR359W / ADE13

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:2.98|P-value:9.75E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:4.66|P-value:6.24E-7 ID:SGTC_53|Compound:0973-0023|FD-Score:3.31|P-value:2.85E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.05|P-value:7.59E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:4.87|P-value:2.06E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:17.50|P-value:1.19E-74 ID:SGTC_68|Compound:0986-0284|FD-Score:3.73|P-value:5.35E-5 ID:SGTC_85|Compound:0868-0259|FD-Score:4.32|P-value:3.43E-6 ID:SGTC_193|Compound:1273-0060|FD-Score:8.99|P-value:4.25E-21 ID:SGTC_198|Compound:3454-2873|FD-Score:4.89|P-value:1.82E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:4.79|P-value:3.08E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:2.98|P-value:9.77E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.06|P-value:7.29E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:2.98|P-value:9.86E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:9.15|P-value:8.35E-22 ID:SGTC_310|Compound:1273-0058|FD-Score:3.75|P-value:4.78E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:4.37|P-value:2.73E-6 ID:SGTC_327|Compound:1273-0057|FD-Score:10.10|P-value:3.17E-26 ID:SGTC_348|Compound:k064-0012|FD-Score:3.81|P-value:3.69E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:14.80|P-value:3.42E-54 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.27|P-value:3.21E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:6.62|P-value:2.81E-12 ID:SGTC_522|Compound:1273-0010|FD-Score:3.33|P-value:2.73E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.26|P-value:3.51E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:3.71|P-value:5.61E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:3.72|P-value:5.43E-5 ID:SGTC_601|Compound:0986-0029|FD-Score:15.60|P-value:1.37E-59 ID:SGTC_609|Compound:0986-0026|FD-Score:12.80|P-value:6.21E-41 ID:SGTC_612|Compound:0302-0167|FD-Score:3.32|P-value:2.77E-4 ID:SGTC_862|Compound:0452-0056|FD-Score:11.90|P-value:2.35E-35 ID:SGTC_884|Compound:0851-0618|FD-Score:3.68|P-value:6.47E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:9.44|P-value:4.24E-23 ID:SGTC_1101|Compound:ebselen|FD-Score:3.10|P-value:6.36E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:5.74|P-value:1.14E-9 ID:SGTC_1260|Compound:0700-1617|FD-Score:-4.52|P-value:1.19E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.66|P-value:6.21E-7 ID:SGTC_1317|Compound:1273-0028|FD-Score:16.40|P-value:7.19E-66 ID:SGTC_1383|Compound:3049-0032|FD-Score:5.28|P-value:1.98E-8 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.53|P-value:1.20E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.47|P-value:1.65E-6 ID:SGTC_1621|Compound:st003705|FD-Score:3.85|P-value:3.07E-5 ID:SGTC_1664|Compound:st014217|FD-Score:3.89|P-value:2.64E-5 ID:SGTC_1675|Compound:st018485|FD-Score:4.07|P-value:1.13E-5 ID:SGTC_1682|Compound:st012968|FD-Score:-5.12|P-value:4.66E-8 ID:SGTC_1756|Compound:st041558|FD-Score:-3.91|P-value:2.21E-5 ID:SGTC_1758|Compound:st042942|FD-Score:3.40|P-value:2.03E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.34|P-value:2.57E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.76|P-value:4.64E-5 ID:SGTC_1767|Compound:st044992|FD-Score:3.24|P-value:3.85E-4 ID:SGTC_1786|Compound:berberine|FD-Score:17.90|P-value:3.26E-78 ID:SGTC_1827|Compound:berberine|FD-Score:9.18|P-value:6.04E-22 ID:SGTC_1829|Compound:berberine|FD-Score:9.77|P-value:1.28E-24 ID:SGTC_1979|Compound:st071809|FD-Score:3.86|P-value:2.98E-5 ID:SGTC_1986|Compound:st071812|FD-Score:-3.89|P-value:2.46E-5 ID:SGTC_1989|Compound:st071868|FD-Score:3.43|P-value:1.84E-4 ID:SGTC_2016|Compound:5121758|FD-Score:5.17|P-value:3.66E-8 ID:SGTC_2131|Compound:toluylene red|FD-Score:4.16|P-value:7.44E-6 ID:SGTC_2135|Compound:5326629|FD-Score:-3.13|P-value:5.33E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.66|P-value:7.10E-5 ID:SGTC_2382|Compound:9035899|FD-Score:3.96|P-value:1.93E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:8.64|P-value:1.16E-19 ID:SGTC_2424|Compound:5537928|FD-Score:5.10|P-value:5.51E-8 ID:SGTC_2460|Compound:5552655|FD-Score:3.61|P-value:8.63E-5 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:5.27|P-value:2.15E-8 ID:SGTC_2511|Compound:berberine|FD-Score:12.10|P-value:1.47E-36 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.30|P-value:3.05E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.80|P-value:3.84E-5 ID:SGTC_2787|Compound:5284540|FD-Score:-3.57|P-value:9.77E-5 ID:SGTC_2790|Compound:5423970|FD-Score:-3.25|P-value:3.44E-4 ID:SGTC_2791|Compound:5663188|FD-Score:4.58|P-value:9.27E-7 ID:SGTC_2874|Compound:9043904|FD-Score:-3.70|P-value:5.68E-5 ID:SGTC_2913|Compound:7966556|FD-Score:4.16|P-value:7.71E-6 ID:SGTC_3026|Compound:9090633|FD-Score:3.41|P-value:2.00E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.54|P-value:1.17E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.64|P-value:7.69E-5 ID:SGTC_3263|Compound:9137018|FD-Score:4.34|P-value:3.11E-6 ID:SGTC_3300|Compound:9124615|FD-Score:3.45|P-value:1.71E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:2.98|P-value:9.75E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:4.66|P-value:6.24E-7 ID:SGTC_53|Compound:0973-0023|FD-Score:3.31|P-value:2.85E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.05|P-value:7.59E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:4.87|P-value:2.06E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:17.50|P-value:1.19E-74 ID:SGTC_68|Compound:0986-0284|FD-Score:3.73|P-value:5.35E-5 ID:SGTC_85|Compound:0868-0259|FD-Score:4.32|P-value:3.43E-6 ID:SGTC_193|Compound:1273-0060|FD-Score:8.99|P-value:4.25E-21 ID:SGTC_198|Compound:3454-2873|FD-Score:4.89|P-value:1.82E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:4.79|P-value:3.08E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:2.98|P-value:9.77E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.06|P-value:7.29E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:2.98|P-value:9.86E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:9.15|P-value:8.35E-22 ID:SGTC_310|Compound:1273-0058|FD-Score:3.75|P-value:4.78E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:4.37|P-value:2.73E-6 ID:SGTC_327|Compound:1273-0057|FD-Score:10.10|P-value:3.17E-26 ID:SGTC_348|Compound:k064-0012|FD-Score:3.81|P-value:3.69E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:14.80|P-value:3.42E-54 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.27|P-value:3.21E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:6.62|P-value:2.81E-12 ID:SGTC_522|Compound:1273-0010|FD-Score:3.33|P-value:2.73E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.26|P-value:3.51E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:3.71|P-value:5.61E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:3.72|P-value:5.43E-5 ID:SGTC_601|Compound:0986-0029|FD-Score:15.60|P-value:1.37E-59 ID:SGTC_609|Compound:0986-0026|FD-Score:12.80|P-value:6.21E-41 ID:SGTC_612|Compound:0302-0167|FD-Score:3.32|P-value:2.77E-4 ID:SGTC_862|Compound:0452-0056|FD-Score:11.90|P-value:2.35E-35 ID:SGTC_884|Compound:0851-0618|FD-Score:3.68|P-value:6.47E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:9.44|P-value:4.24E-23 ID:SGTC_1101|Compound:ebselen|FD-Score:3.10|P-value:6.36E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:5.74|P-value:1.14E-9 ID:SGTC_1260|Compound:0700-1617|FD-Score:-4.52|P-value:1.19E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.66|P-value:6.21E-7 ID:SGTC_1317|Compound:1273-0028|FD-Score:16.40|P-value:7.19E-66 ID:SGTC_1383|Compound:3049-0032|FD-Score:5.28|P-value:1.98E-8 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.53|P-value:1.20E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.47|P-value:1.65E-6 ID:SGTC_1621|Compound:st003705|FD-Score:3.85|P-value:3.07E-5 ID:SGTC_1664|Compound:st014217|FD-Score:3.89|P-value:2.64E-5 ID:SGTC_1675|Compound:st018485|FD-Score:4.07|P-value:1.13E-5 ID:SGTC_1682|Compound:st012968|FD-Score:-5.12|P-value:4.66E-8 ID:SGTC_1756|Compound:st041558|FD-Score:-3.91|P-value:2.21E-5 ID:SGTC_1758|Compound:st042942|FD-Score:3.40|P-value:2.03E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.34|P-value:2.57E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.76|P-value:4.64E-5 ID:SGTC_1767|Compound:st044992|FD-Score:3.24|P-value:3.85E-4 ID:SGTC_1786|Compound:berberine|FD-Score:17.90|P-value:3.26E-78 ID:SGTC_1827|Compound:berberine|FD-Score:9.18|P-value:6.04E-22 ID:SGTC_1829|Compound:berberine|FD-Score:9.77|P-value:1.28E-24 ID:SGTC_1979|Compound:st071809|FD-Score:3.86|P-value:2.98E-5 ID:SGTC_1986|Compound:st071812|FD-Score:-3.89|P-value:2.46E-5 ID:SGTC_1989|Compound:st071868|FD-Score:3.43|P-value:1.84E-4 ID:SGTC_2016|Compound:5121758|FD-Score:5.17|P-value:3.66E-8 ID:SGTC_2131|Compound:toluylene red|FD-Score:4.16|P-value:7.44E-6 ID:SGTC_2135|Compound:5326629|FD-Score:-3.13|P-value:5.33E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.66|P-value:7.10E-5 ID:SGTC_2382|Compound:9035899|FD-Score:3.96|P-value:1.93E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:8.64|P-value:1.16E-19 ID:SGTC_2424|Compound:5537928|FD-Score:5.10|P-value:5.51E-8 ID:SGTC_2460|Compound:5552655|FD-Score:3.61|P-value:8.63E-5 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:5.27|P-value:2.15E-8 ID:SGTC_2511|Compound:berberine|FD-Score:12.10|P-value:1.47E-36 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.30|P-value:3.05E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.80|P-value:3.84E-5 ID:SGTC_2787|Compound:5284540|FD-Score:-3.57|P-value:9.77E-5 ID:SGTC_2790|Compound:5423970|FD-Score:-3.25|P-value:3.44E-4 ID:SGTC_2791|Compound:5663188|FD-Score:4.58|P-value:9.27E-7 ID:SGTC_2874|Compound:9043904|FD-Score:-3.70|P-value:5.68E-5 ID:SGTC_2913|Compound:7966556|FD-Score:4.16|P-value:7.71E-6 ID:SGTC_3026|Compound:9090633|FD-Score:3.41|P-value:2.00E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.54|P-value:1.17E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.64|P-value:7.69E-5 ID:SGTC_3263|Compound:9137018|FD-Score:4.34|P-value:3.11E-6 ID:SGTC_3300|Compound:9124615|FD-Score:3.45|P-value:1.71E-4

Top fitness defect scores for YLR359W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1786 berberine 17.90 21.6 µM 3.26E-78 60S ribosome export 1 2.72E-72 4.99
2 SGTC_63 0099-0242 17.50 26.7 µM 1.19E-74 mitochondrial stress 1 5.24E-69 4.30
3 SGTC_1317 1273-0028 16.40 910.0 nM 7.19E-66 azole & statin 2 6.54E-61 7.56
4 SGTC_601 0986-0029 15.60 62.9 µM 1.37E-59 calcium & mitochondrial duress 1 4.03E-55 2.72
5 SGTC_363 0455-0021 14.80 3.1 µM 3.42E-54 azole & statin 2 3.83E-50 3.70
6 SGTC_609 0986-0026 12.80 86.3 µM 6.21E-41 calcium & mitochondrial duress 1 6.47E-38 3.71
7 SGTC_2511 berberine 12.10 10.5 µM 1.47E-36 60S ribosome export 1 7.00E-34 4.29
8 SGTC_862 0452-0056 11.90 10.2 µM 2.35E-35 azole & statin 1 9.04E-33 7.26
9 SGTC_327 1273-0057 10.10 4.0 µM 3.17E-26 azole & statin 1 2.39E-24 4.09
10 SGTC_1829 berberine 9.77 14.4 µM 1.28E-24 60S ribosome export 3 7.25E-23 0.11
11 SGTC_1083 didemethylchlorpromazine sulfoxide 9.44 707.4 nM 4.24E-23 redox potentiating 8 1.84E-21 1.69
12 SGTC_1827 berberine 9.18 7.2 µM 6.04E-22 60S ribosome export 3 2.13E-20 1.83
13 SGTC_299 1273-0059 9.15 1.4 µM 8.35E-22 1 2.87E-20 2.10
14 SGTC_193 1273-0060 8.99 1.6 µM 4.25E-21 ergosterol biosynthesis 2 1.29E-19 3.42
15 SGTC_2418 pentamidine 8.64 31.7 µM 1.16E-19 mitochondrial processes 3 2.73E-18 0.08
16 SGTC_425 3825-7017 6.62 13.1 µM 2.81E-12 3 1.80E-11 1.24
17 SGTC_1257 0669-0121 5.74 16.0 µM 1.14E-9 1 4.63E-9 1.70
18 SGTC_1383 3049-0032 5.28 20.7 µM 1.98E-8 60S ribosome export 3 6.46E-8 0.27
19 SGTC_2501 sodium tetradecyl sulfate 5.27 13.4 µM 2.15E-8 2 6.98E-8 1.08
20 SGTC_2016 5121758 5.17 140.0 µM 3.66E-8 redox potentiating 8 1.14E-7 0.26

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.376 2.49E-113 YDR181C SAS4 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.359 2.09E-102 YPR198W SGE1 hom Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
0.280 1.17E-61 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.277 3.15E-60 YPR197C_d YPR197C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.241 2.15E-45 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.198 5.48E-31 YHR072W ERG7 het Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
0.189 3.13E-28 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.186 1.77E-27 YML081W TDA9 hom DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
0.185 2.38E-27 YOR072W_d YOR072W_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
0.173 4.63E-24 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.173 6.38E-24 YHR113W APE4 hom Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
0.164 1.26E-21 YGR004W PEX31 hom Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
0.158 4.74E-20 YMR063W RIM9 hom Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
0.156 1.04E-19 YOR213C SAS5 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
0.155 1.45E-19 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH