HIPHOP chemogenomics database

YLR359W / ADE13

Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency

Zygosity: Heterozygous strain

fixedexpanded
Profile for YLR359W / ADE13

Click on Significant Values for Screen Details

Top fitness defect scores for YLR359W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1786	berberine	17.90	21.6 µM	3.26E-78	60S ribosome export	1	2.72E-72	4.99
2	SGTC_63	0099-0242	17.50	26.7 µM	1.19E-74	mitochondrial stress	1	5.24E-69	4.30
3	SGTC_1317	1273-0028	16.40	910.0 nM	7.19E-66	azole & statin	2	6.54E-61	7.56
4	SGTC_601	0986-0029	15.60	62.9 µM	1.37E-59	calcium & mitochondrial duress	1	4.03E-55	2.72
5	SGTC_363	0455-0021	14.80	3.1 µM	3.42E-54	azole & statin	2	3.83E-50	3.70
6	SGTC_609	0986-0026	12.80	86.3 µM	6.21E-41	calcium & mitochondrial duress	1	6.47E-38	3.71
7	SGTC_2511	berberine	12.10	10.5 µM	1.47E-36	60S ribosome export	1	7.00E-34	4.29
8	SGTC_862	0452-0056	11.90	10.2 µM	2.35E-35	azole & statin	1	9.04E-33	7.26
9	SGTC_327	1273-0057	10.10	4.0 µM	3.17E-26	azole & statin	1	2.39E-24	4.09
10	SGTC_1829	berberine	9.77	14.4 µM	1.28E-24	60S ribosome export	3	7.25E-23	0.11
11	SGTC_1083	didemethylchlorpromazine sulfoxide	9.44	707.4 nM	4.24E-23	redox potentiating	8	1.84E-21	1.69
12	SGTC_1827	berberine	9.18	7.2 µM	6.04E-22	60S ribosome export	3	2.13E-20	1.83
13	SGTC_299	1273-0059	9.15	1.4 µM	8.35E-22		1	2.87E-20	2.10
14	SGTC_193	1273-0060	8.99	1.6 µM	4.25E-21	ergosterol biosynthesis	2	1.29E-19	3.42
15	SGTC_2418	pentamidine	8.64	31.7 µM	1.16E-19	mitochondrial processes	3	2.73E-18	0.08
16	SGTC_425	3825-7017	6.62	13.1 µM	2.81E-12		3	1.80E-11	1.24
17	SGTC_1257	0669-0121	5.74	16.0 µM	1.14E-9		1	4.63E-9	1.70
18	SGTC_1383	3049-0032	5.28	20.7 µM	1.98E-8	60S ribosome export	3	6.46E-8	0.27
19	SGTC_2501	sodium tetradecyl sulfate	5.27	13.4 µM	2.15E-8		2	6.98E-8	1.08
20	SGTC_2016	5121758	5.17	140.0 µM	3.66E-8	redox potentiating	8	1.14E-7	0.26

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.376	2.49E-113	YDR181C	SAS4	hom	Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.359	2.09E-102	YPR198W	SGE1	hom	Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
0.280	1.17E-61	YMR127C	SAS2	hom	Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.277	3.15E-60	YPR197C_d	YPR197C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.241	2.15E-45	YMR154C	RIM13	hom	Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.198	5.48E-31	YHR072W	ERG7	het	Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
0.189	3.13E-28	YGL045W	RIM8	hom	Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.186	1.77E-27	YML081W	TDA9	hom	DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
0.185	2.38E-27	YOR072W_d	YOR072W_d	hom	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
0.173	4.63E-24	YOR275C	RIM20	hom	Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.173	6.38E-24	YHR113W	APE4	hom	Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family
0.164	1.26E-21	YGR004W	PEX31	hom	Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
0.158	4.74E-20	YMR063W	RIM9	hom	Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
0.156	1.04E-19	YOR213C	SAS5	hom	Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
0.155	1.45E-19	YNL294C	RIM21	hom	pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH