YLR363C / NMD4

Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR363C / NMD4

Click on Significant Values for Screen Details ID:SGTC_82|Compound:0054-0107|FD-Score:3.87|P-value:4.83E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.61|P-value:1.35E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:4.99|P-value:2.52E-7 ID:SGTC_359|Compound:0081-0058|FD-Score:4.43|P-value:4.07E-6 ID:SGTC_466|Compound:geldanamycin|FD-Score:-4.84|P-value:5.28E-7 ID:SGTC_762|Compound:1165-0429|FD-Score:3.46|P-value:2.49E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-4.59|P-value:1.83E-6 ID:SGTC_927|Compound:2425-1185|FD-Score:3.62|P-value:1.35E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.52|P-value:2.00E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-4.61|P-value:1.72E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:6.10|P-value:3.91E-10 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.41|P-value:2.93E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.76|P-value:7.70E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.67|P-value:1.07E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.55|P-value:1.74E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:-3.28|P-value:4.68E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.37|P-value:3.49E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.58|P-value:1.51E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.13|P-value:8.04E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-3.11|P-value:8.58E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.61|P-value:1.39E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.25|P-value:5.35E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-3.62|P-value:1.32E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-5.51|P-value:1.40E-8 ID:SGTC_1750|Compound:st081859|FD-Score:5.56|P-value:1.08E-8 ID:SGTC_1863|Compound:st057641|FD-Score:3.40|P-value:3.10E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:6.02|P-value:6.83E-10 ID:SGTC_1888|Compound:azatryptophan|FD-Score:-4.40|P-value:4.49E-6 ID:SGTC_2066|Compound:5222451|FD-Score:-3.60|P-value:1.42E-4 ID:SGTC_2127|Compound:5253358|FD-Score:-3.10|P-value:8.79E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-4.22|P-value:1.07E-5 ID:SGTC_2258|Compound:7960471|FD-Score:3.25|P-value:5.28E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.10|P-value:9.05E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:5.40|P-value:2.62E-8 ID:SGTC_2464|Compound:5717415|FD-Score:4.02|P-value:2.62E-5 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.22|P-value:1.09E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.74|P-value:8.26E-5 ID:SGTC_2837|Compound:9008476|FD-Score:-3.29|P-value:4.56E-4 ID:SGTC_3151|Compound:9099821|FD-Score:5.02|P-value:2.19E-7 ID:SGTC_3356|Compound:9151811|FD-Score:-3.74|P-value:8.19E-5 ID:SGTC_82|Compound:0054-0107|FD-Score:3.87|P-value:4.83E-5 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.61|P-value:1.35E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:4.99|P-value:2.52E-7 ID:SGTC_359|Compound:0081-0058|FD-Score:4.43|P-value:4.07E-6 ID:SGTC_466|Compound:geldanamycin|FD-Score:-4.84|P-value:5.28E-7 ID:SGTC_762|Compound:1165-0429|FD-Score:3.46|P-value:2.49E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-4.59|P-value:1.83E-6 ID:SGTC_927|Compound:2425-1185|FD-Score:3.62|P-value:1.35E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.52|P-value:2.00E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-4.61|P-value:1.72E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:6.10|P-value:3.91E-10 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.41|P-value:2.93E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.76|P-value:7.70E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.67|P-value:1.07E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.55|P-value:1.74E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:-3.28|P-value:4.68E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.37|P-value:3.49E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.58|P-value:1.51E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.13|P-value:8.04E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-3.11|P-value:8.58E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.61|P-value:1.39E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.25|P-value:5.35E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-3.62|P-value:1.32E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-5.51|P-value:1.40E-8 ID:SGTC_1750|Compound:st081859|FD-Score:5.56|P-value:1.08E-8 ID:SGTC_1863|Compound:st057641|FD-Score:3.40|P-value:3.10E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:6.02|P-value:6.83E-10 ID:SGTC_1888|Compound:azatryptophan|FD-Score:-4.40|P-value:4.49E-6 ID:SGTC_2066|Compound:5222451|FD-Score:-3.60|P-value:1.42E-4 ID:SGTC_2127|Compound:5253358|FD-Score:-3.10|P-value:8.79E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-4.22|P-value:1.07E-5 ID:SGTC_2258|Compound:7960471|FD-Score:3.25|P-value:5.28E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.10|P-value:9.05E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:5.40|P-value:2.62E-8 ID:SGTC_2464|Compound:5717415|FD-Score:4.02|P-value:2.62E-5 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.22|P-value:1.09E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.74|P-value:8.26E-5 ID:SGTC_2837|Compound:9008476|FD-Score:-3.29|P-value:4.56E-4 ID:SGTC_3151|Compound:9099821|FD-Score:5.02|P-value:2.19E-7 ID:SGTC_3356|Compound:9151811|FD-Score:-3.74|P-value:8.19E-5

Top fitness defect scores for YLR363C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1137 ixabepilone 6.10 100.0 µM 3.91E-10 6 5.19E-10
2 SGTC_1877 wortmannin 6.02 25.0 µM 6.83E-10 47 8.97E-10
3 SGTC_1750 st081859 5.56 2.4 µM 1.08E-8 endomembrane recycling 118 1.36E-8
4 SGTC_2408 sulbentine 5.40 18.4 µM 2.62E-8 26 3.27E-8
5 SGTC_3151 9099821 5.02 49.5 µM 2.19E-7 7 2.65E-7
6 SGTC_344 k064-0035 4.99 6.4 µM 2.52E-7 60S ribosome export 11 3.05E-7
7 SGTC_359 0081-0058 4.43 15.9 µM 4.07E-6 60S ribosome export 28 4.72E-6
8 SGTC_2675 theaflavin digallate 4.22 100.0 µM 1.09E-5 iron homeostasis 25 1.24E-5
9 SGTC_2464 5717415 4.02 7.7 µM 2.62E-5 16 2.96E-5
10 SGTC_82 0054-0107 3.87 293.8 µM 4.83E-5 11 5.41E-5
11 SGTC_1349 flufenamic acid 3.76 69.0 µM 7.70E-5 17 8.57E-5
12 SGTC_1396 3511-0007 3.67 98.6 µM 1.07E-4 mitochondrial processes 18 1.19E-4
13 SGTC_927 2425-1185 3.62 123.0 µM 1.35E-4 38 1.49E-4
14 SGTC_1675 st018485 3.61 24.5 µM 1.39E-4 60S ribosome export 39 1.53E-4
15 SGTC_1462 k081-0014 3.55 26.5 µM 1.74E-4 13 1.92E-4
16 SGTC_1011 4112-3381 3.52 17.4 µM 2.00E-4 59 2.19E-4
17 SGTC_762 1165-0429 3.46 115.0 µM 2.49E-4 TSC3-RPN4 34 2.73E-4
18 SGTC_1175 0741-0056 3.41 27.6 µM 2.93E-4 43 3.20E-4
19 SGTC_1863 st057641 3.40 20.1 µM 3.10E-4 30 3.38E-4
20 SGTC_1538 2',3',6-trimethoxyflavanone 3.37 63.6 µM 3.49E-4 29 3.81E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 3.08E-23 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.151 1.60E-18 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.141 2.91E-16 YPR153W YPR153W hom Putative protein of unknown function
0.133 1.04E-14 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.132 1.83E-14 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.122 1.12E-12 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.122 1.30E-12 YGR009C SEC9 het t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
0.118 7.37E-12 YML072C TCB3 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.102 3.22E-9 YGL227W VID30 hom Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
0.101 4.05E-9 YJL026W RNR2 het Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
0.101 4.21E-9 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.098 1.25E-8 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.093 6.38E-8 YHR151C MTC6 hom Protein of unknown function; mtc6 is synthetically sick with cdc13-1
0.092 1.06E-7 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.086 6.45E-7 YBR255W MTC4 hom Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1