Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress

Zygosity: Homozygous strain
Profile for YLR363W-A / YLR363W-A

Click on Significant Values for Screen Details

Top fitness defect scores for YLR363W-A deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1129 3453-1384 6.47 223.0 µM 2.83E-13 8 4.99E-11
2 SGTC_946 3434-1334 5.77 87.4 µM 5.91E-11 5 3.87E-9
3 SGTC_945 3381-0768 4.89 11.3 µM 2.27E-8 endomembrane recycling 16 4.94E-7
4 SGTC_803 4488-1585 4.85 147.0 µM 2.95E-8 3 6.12E-7
5 SGTC_1136 pergolide 4.82 1.1 µM 3.55E-8 18 7.10E-7
6 SGTC_1334 1486-0610 4.78 236.0 µM 4.53E-8 60S ribosome export 6 8.67E-7
7 SGTC_939 1165-0479 4.27 45.3 µM 9.00E-7 19 9.91E-6
8 SGTC_80 0159-0065 3.46 512.2 µM 5.13E-5 7 2.68E-4
9 SGTC_1678 st019265 3.42 80.2 µM 6.23E-5 60S ribosome export 41 3.14E-4
10 SGTC_1284 0929-0043 3.34 16.0 µM 8.73E-5 51 4.14E-4
11 SGTC_2395 5646831 3.31 180.2 µM 1.01E-4 49 4.67E-4
12 SGTC_1894 3',6'-dihydroxyflavone 3.29 78.7 µM 1.10E-4 31 4.99E-4
13 SGTC_1269 0831-0796 3.28 3.0 µM 1.16E-4 60S ribosome export 33 5.21E-4
14 SGTC_2625 euphol 3.24 100.0 µM 1.39E-4 63 6.04E-4
15 SGTC_2643 anethole 3.23 100.0 µM 1.45E-4 40 6.25E-4
16 SGTC_2856 9022925 3.22 19.5 µM 1.46E-4 53 6.31E-4
17 SGTC_1762 st045503 3.11 25.5 µM 2.34E-4 60 9.27E-4
18 SGTC_2476 5806916 3.08 10.5 µM 2.63E-4 45 0.00102
19 SGTC_97 0535-0701 3.02 19.1 µM 3.45E-4 60S ribosome export 58 0.00127
20 SGTC_2859 9028643 3.01 45.5 µM 3.54E-4 46 0.00130

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.195 3.03E-30 YMR095C SNO1 hom Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
0.150 1.88E-18 YER109C FLO8 hom Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene
0.149 4.94E-18 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.143 8.68E-17 YDR513W GRX2 hom Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
0.137 1.44E-15 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.133 9.16E-15 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.132 1.89E-14 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.113 4.62E-11 YKL141W SDH3 het Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
0.113 5.55E-11 YAL062W GDH3 hom NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
0.110 1.51E-10 YJR035W RAD26 hom Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
0.109 2.30E-10 YML116W-A_d YML116W-A_d hom Putative protein of unknown function
0.109 2.39E-10 YKL022C CDC16 het Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
0.108 3.58E-10 YCR072C RSA4 het WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
0.106 6.60E-10 YMR199W CLN1 hom G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
0.105 9.54E-10 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p