YLR364W / GRX8

Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR364W / GRX8

Click on Significant Values for Screen Details ID:SGTC_168|Compound:3970-0862|FD-Score:-3.13|P-value:3.43E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.89|P-value:2.98E-8 ID:SGTC_323|Compound:4013-0906|FD-Score:3.14|P-value:2.38E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.31|P-value:1.15E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-2.93|P-value:7.54E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.18|P-value:2.66E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:3.62|P-value:2.89E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.42|P-value:6.95E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:2.84|P-value:7.61E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.11|P-value:2.61E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.02|P-value:3.76E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-2.95|P-value:6.96E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.49|P-value:5.26E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.43|P-value:6.62E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.96|P-value:4.87E-4 ID:SGTC_1115|Compound:galangin|FD-Score:4.11|P-value:2.45E-6 ID:SGTC_1227|Compound:3909-8010|FD-Score:2.84|P-value:7.57E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:3.48|P-value:5.49E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:4.59|P-value:1.79E-7 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.30|P-value:1.22E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:2.86|P-value:7.13E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:-3.03|P-value:5.05E-4 ID:SGTC_1523|Compound:st012844|FD-Score:2.80|P-value:9.06E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:4.38|P-value:5.79E-7 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.37|P-value:8.92E-5 ID:SGTC_1699|Compound:st031318|FD-Score:3.45|P-value:6.13E-5 ID:SGTC_1942|Compound:st076330|FD-Score:-4.11|P-value:3.83E-6 ID:SGTC_2022|Compound:5136654|FD-Score:3.03|P-value:3.65E-4 ID:SGTC_2280|Compound:7785178|FD-Score:2.88|P-value:6.54E-4 ID:SGTC_2288|Compound:7946289|FD-Score:2.83|P-value:8.06E-4 ID:SGTC_2354|Compound:9060492|FD-Score:2.78|P-value:9.63E-4 ID:SGTC_2437|Compound:5741531|FD-Score:-3.29|P-value:1.79E-4 ID:SGTC_2517|Compound:totarol|FD-Score:2.77|P-value:9.91E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.81|P-value:1.14E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.04|P-value:3.46E-4 ID:SGTC_2888|Compound:9058547|FD-Score:2.94|P-value:5.33E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.37|P-value:8.80E-5 ID:SGTC_3072|Compound:9116419|FD-Score:3.02|P-value:3.75E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-2.99|P-value:5.89E-4 ID:SGTC_3320|Compound:9137005|FD-Score:3.55|P-value:3.98E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.13|P-value:3.43E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.89|P-value:2.98E-8 ID:SGTC_323|Compound:4013-0906|FD-Score:3.14|P-value:2.38E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.31|P-value:1.15E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-2.93|P-value:7.54E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.18|P-value:2.66E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:3.62|P-value:2.89E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.42|P-value:6.95E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:2.84|P-value:7.61E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.11|P-value:2.61E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.02|P-value:3.76E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-2.95|P-value:6.96E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.49|P-value:5.26E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.43|P-value:6.62E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.96|P-value:4.87E-4 ID:SGTC_1115|Compound:galangin|FD-Score:4.11|P-value:2.45E-6 ID:SGTC_1227|Compound:3909-8010|FD-Score:2.84|P-value:7.57E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:3.48|P-value:5.49E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:4.59|P-value:1.79E-7 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.30|P-value:1.22E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:2.86|P-value:7.13E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:-3.03|P-value:5.05E-4 ID:SGTC_1523|Compound:st012844|FD-Score:2.80|P-value:9.06E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:4.38|P-value:5.79E-7 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.37|P-value:8.92E-5 ID:SGTC_1699|Compound:st031318|FD-Score:3.45|P-value:6.13E-5 ID:SGTC_1942|Compound:st076330|FD-Score:-4.11|P-value:3.83E-6 ID:SGTC_2022|Compound:5136654|FD-Score:3.03|P-value:3.65E-4 ID:SGTC_2280|Compound:7785178|FD-Score:2.88|P-value:6.54E-4 ID:SGTC_2288|Compound:7946289|FD-Score:2.83|P-value:8.06E-4 ID:SGTC_2354|Compound:9060492|FD-Score:2.78|P-value:9.63E-4 ID:SGTC_2437|Compound:5741531|FD-Score:-3.29|P-value:1.79E-4 ID:SGTC_2517|Compound:totarol|FD-Score:2.77|P-value:9.91E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.81|P-value:1.14E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.04|P-value:3.46E-4 ID:SGTC_2888|Compound:9058547|FD-Score:2.94|P-value:5.33E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.37|P-value:8.80E-5 ID:SGTC_3072|Compound:9116419|FD-Score:3.02|P-value:3.75E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-2.99|P-value:5.89E-4 ID:SGTC_3320|Compound:9137005|FD-Score:3.55|P-value:3.98E-5

Top fitness defect scores for YLR364W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_312 4092-0653 4.89 6.8 µM 2.98E-8 ergosterol depletion effects on membrane 60 5.09E-7
2 SGTC_1469 k284-2957 4.59 126.0 µM 1.79E-7 5 2.23E-6
3 SGTC_1589 2',5'-dimethoxyflavone 4.38 47.2 µM 5.79E-7 8 5.90E-6
4 SGTC_1115 galangin 4.11 695.7 nM 2.45E-6 RPP1 & pyrimidine depletion 10 1.94E-5
5 SGTC_2539 epi(13)torulosol 3.81 80.0 µM 1.14E-5 31 6.94E-5
6 SGTC_430 0958-0115 3.62 16.4 µM 2.89E-5 36 1.49E-4
7 SGTC_3320 9137005 3.55 35.1 µM 3.98E-5 endomembrane recycling 24 1.95E-4
8 SGTC_890 0929-0006 3.49 47.2 µM 5.26E-5 24 2.45E-4
9 SGTC_1235 benzylparaben 3.48 21.5 µM 5.49E-5 22 2.54E-4
10 SGTC_1699 st031318 3.45 29.1 µM 6.13E-5 35 2.78E-4
11 SGTC_1083 didemethylchlorpromazine sulfoxide 3.43 707.4 nM 6.62E-5 redox potentiating 111 2.97E-4
12 SGTC_473 2-arachidonoylglycerol 3.42 10.0 µM 6.95E-5 29 3.09E-4
13 SGTC_2928 9011484 3.37 9.8 µM 8.80E-5 heme biosynthesis & mitochondrial translocase 55 3.75E-4
14 SGTC_1596 piperlongumine 3.37 63.0 µM 8.92E-5 35 3.79E-4
15 SGTC_363 0455-0021 3.31 3.1 µM 1.15E-4 azole & statin 103 4.67E-4
16 SGTC_1473 k844-0725 3.30 134.0 µM 1.22E-4 37 4.90E-4
17 SGTC_183 3914-0051 3.19 30.6 µM 1.87E-4 60S ribosome export 51 7.00E-4
18 SGTC_323 4013-0906 3.14 34.3 µM 2.38E-4 ERG2 29 8.56E-4
19 SGTC_705 1124-0212 3.11 310.0 µM 2.61E-4 46 9.23E-4
20 SGTC_2788 5282734 3.04 71.4 µM 3.46E-4 99 0.00117

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.178 2.84E-25 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.151 1.77E-18 YDR144C MKC7 hom GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p
0.139 6.60E-16 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.128 9.30E-14 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.107 6.12E-10 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.103 2.51E-9 YGR274C TAF1 het TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
0.101 5.08E-9 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.101 5.18E-9 YPR160W GPH1 hom Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway
0.099 8.91E-9 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.098 1.14E-8 YER180C-A SLO1 hom Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
0.091 1.48E-7 YOR338W_p YOR338W_p hom Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication
0.090 1.76E-7 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.088 2.82E-7 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.087 4.92E-7 YFL015C_d YFL015C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
0.086 5.70E-7 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability