YLR371W / ROM2

GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR371W / ROM2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.48|P-value:6.65E-6 ID:SGTC_67|Compound:0335-0847|FD-Score:8.80|P-value:1.43E-9 ID:SGTC_70|Compound:0141-0289|FD-Score:5.22|P-value:2.38E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:5.74|P-value:5.85E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:5.51|P-value:1.12E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.66|P-value:9.15E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:6.24|P-value:1.40E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:5.04|P-value:3.70E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:4.63|P-value:9.82E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-5.60|P-value:5.00E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.18|P-value:1.59E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:6.84|P-value:2.13E-6 ID:SGTC_590|Compound:k048-0076|FD-Score:6.05|P-value:2.41E-5 ID:SGTC_622|Compound:1348-1566|FD-Score:4.97|P-value:4.41E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:-4.88|P-value:3.43E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:8.53|P-value:4.42E-9 ID:SGTC_674|Compound:0498-0004|FD-Score:-4.70|P-value:5.27E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.00|P-value:4.09E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.37|P-value:1.62E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:5.75|P-value:5.72E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:4.95|P-value:4.65E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-5.06|P-value:2.14E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:-5.25|P-value:1.31E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:4.71|P-value:8.29E-4 ID:SGTC_1616|Compound:st002734|FD-Score:-5.02|P-value:2.38E-4 ID:SGTC_1687|Compound:st020778|FD-Score:6.92|P-value:1.60E-6 ID:SGTC_1834|Compound:st055981|FD-Score:4.63|P-value:9.85E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:6.36|P-value:9.63E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:-4.46|P-value:9.34E-4 ID:SGTC_1959|Compound:st077091|FD-Score:6.22|P-value:1.47E-5 ID:SGTC_2023|Compound:5144124|FD-Score:-5.53|P-value:6.16E-5 ID:SGTC_2092|Compound:5303694|FD-Score:4.71|P-value:8.15E-4 ID:SGTC_2179|Compound:6298986|FD-Score:5.92|P-value:3.56E-5 ID:SGTC_2254|Compound:7938975|FD-Score:-4.73|P-value:4.93E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.03|P-value:3.85E-4 ID:SGTC_2399|Compound:5884133|FD-Score:5.58|P-value:9.05E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-6.62|P-value:2.27E-6 ID:SGTC_2551|Compound:apiole|FD-Score:-5.54|P-value:5.99E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:7.68|P-value:1.15E-7 ID:SGTC_2584|Compound:androsterone|FD-Score:6.25|P-value:1.35E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:-5.60|P-value:5.03E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:4.98|P-value:4.36E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-6.14|P-value:1.05E-5 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:4.81|P-value:6.55E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-4.59|P-value:6.97E-4 ID:SGTC_2912|Compound:7964298|FD-Score:7.67|P-value:1.17E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.81|P-value:4.10E-4 ID:SGTC_3259|Compound:9138485|FD-Score:-6.38|P-value:4.98E-6 ID:SGTC_3266|Compound:9136860|FD-Score:10.90|P-value:7.38E-14 ID:SGTC_3285|Compound:9112818|FD-Score:5.21|P-value:2.45E-4 ID:SGTC_3303|Compound:9125618|FD-Score:-5.22|P-value:1.41E-4 ID:SGTC_3308|Compound:9128332|FD-Score:-5.86|P-value:2.37E-5 ID:SGTC_3311|Compound:9131146|FD-Score:7.14|P-value:7.60E-7 ID:SGTC_3321|Compound:9137793|FD-Score:7.25|P-value:5.29E-7 ID:SGTC_3322|Compound:9139058|FD-Score:5.21|P-value:2.40E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.48|P-value:6.65E-6 ID:SGTC_67|Compound:0335-0847|FD-Score:8.80|P-value:1.43E-9 ID:SGTC_70|Compound:0141-0289|FD-Score:5.22|P-value:2.38E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:5.74|P-value:5.85E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:5.51|P-value:1.12E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.66|P-value:9.15E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:6.24|P-value:1.40E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:5.04|P-value:3.70E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:4.63|P-value:9.82E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-5.60|P-value:5.00E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.18|P-value:1.59E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:6.84|P-value:2.13E-6 ID:SGTC_590|Compound:k048-0076|FD-Score:6.05|P-value:2.41E-5 ID:SGTC_622|Compound:1348-1566|FD-Score:4.97|P-value:4.41E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:-4.88|P-value:3.43E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:8.53|P-value:4.42E-9 ID:SGTC_674|Compound:0498-0004|FD-Score:-4.70|P-value:5.27E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.00|P-value:4.09E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.37|P-value:1.62E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:5.75|P-value:5.72E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:4.95|P-value:4.65E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-5.06|P-value:2.14E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:-5.25|P-value:1.31E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:4.71|P-value:8.29E-4 ID:SGTC_1616|Compound:st002734|FD-Score:-5.02|P-value:2.38E-4 ID:SGTC_1687|Compound:st020778|FD-Score:6.92|P-value:1.60E-6 ID:SGTC_1834|Compound:st055981|FD-Score:4.63|P-value:9.85E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:6.36|P-value:9.63E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:-4.46|P-value:9.34E-4 ID:SGTC_1959|Compound:st077091|FD-Score:6.22|P-value:1.47E-5 ID:SGTC_2023|Compound:5144124|FD-Score:-5.53|P-value:6.16E-5 ID:SGTC_2092|Compound:5303694|FD-Score:4.71|P-value:8.15E-4 ID:SGTC_2179|Compound:6298986|FD-Score:5.92|P-value:3.56E-5 ID:SGTC_2254|Compound:7938975|FD-Score:-4.73|P-value:4.93E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.03|P-value:3.85E-4 ID:SGTC_2399|Compound:5884133|FD-Score:5.58|P-value:9.05E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-6.62|P-value:2.27E-6 ID:SGTC_2551|Compound:apiole|FD-Score:-5.54|P-value:5.99E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:7.68|P-value:1.15E-7 ID:SGTC_2584|Compound:androsterone|FD-Score:6.25|P-value:1.35E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:-5.60|P-value:5.03E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:4.98|P-value:4.36E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-6.14|P-value:1.05E-5 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:4.81|P-value:6.55E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-4.59|P-value:6.97E-4 ID:SGTC_2912|Compound:7964298|FD-Score:7.67|P-value:1.17E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.81|P-value:4.10E-4 ID:SGTC_3259|Compound:9138485|FD-Score:-6.38|P-value:4.98E-6 ID:SGTC_3266|Compound:9136860|FD-Score:10.90|P-value:7.38E-14 ID:SGTC_3285|Compound:9112818|FD-Score:5.21|P-value:2.45E-4 ID:SGTC_3303|Compound:9125618|FD-Score:-5.22|P-value:1.41E-4 ID:SGTC_3308|Compound:9128332|FD-Score:-5.86|P-value:2.37E-5 ID:SGTC_3311|Compound:9131146|FD-Score:7.14|P-value:7.60E-7 ID:SGTC_3321|Compound:9137793|FD-Score:7.25|P-value:5.29E-7 ID:SGTC_3322|Compound:9139058|FD-Score:5.21|P-value:2.40E-4

Top fitness defect scores for YLR371W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3266 9136860 10.90 49.5 µM 7.38E-14 cell wall 5 4.35E-28
2 SGTC_67 0335-0847 8.80 266.0 µM 1.43E-9 ubiquinone biosynthesis & proteasome 32 6.83E-19
3 SGTC_625 k213-0058 8.53 121.0 µM 4.42E-9 translation 8 7.58E-18
4 SGTC_2555 cholic acid 7.68 100.0 µM 1.15E-7 1 8.02E-15
5 SGTC_2912 7964298 7.67 10.9 µM 1.17E-7 cell wall 28 8.42E-15
6 SGTC_3321 9137793 7.25 26.8 µM 5.29E-7 cell wall 18 2.09E-13
7 SGTC_3311 9131146 7.14 2.9 µM 7.60E-7 cell wall 44 4.54E-13
8 SGTC_1687 st020778 6.92 55.4 µM 1.60E-6 89 2.21E-12
9 SGTC_586 0957-0265 6.84 8.2 µM 2.13E-6 cell wall 12 4.09E-12
10 SGTC_58 0957-0393 6.48 9.0 µM 6.65E-6 cell wall 184 4.60E-11
11 SGTC_1874 staurosporine 6.36 7.6 µM 9.63E-6 cell wall signaling 9 1.01E-10
12 SGTC_2584 androsterone 6.25 48.7 µM 1.35E-5 cell wall 52 2.08E-10
13 SGTC_360 0957-0399 6.24 1.0 µM 1.40E-5 cell wall 10 2.24E-10
14 SGTC_1959 st077091 6.22 55.8 µM 1.47E-5 RPP1 & pyrimidine depletion 6 2.48E-10
15 SGTC_590 k048-0076 6.05 22.4 µM 2.41E-5 RSC & ERG11 2 7.13E-10
16 SGTC_2179 6298986 5.92 200.0 µM 3.56E-5 heme requiring 4 1.63E-9
17 SGTC_928 2764-0428 5.75 58.7 µM 5.72E-5 cell wall 18 4.44E-9
18 SGTC_138 0850-0259 5.74 40.9 µM 5.85E-5 cell wall 114 4.67E-9
19 SGTC_2399 5884133 5.58 200.0 µM 9.05E-5 cell wall signaling 31 1.17E-8
20 SGTC_351 caspofungin 5.51 25.0 nM 1.12E-4 cell wall 46 1.83E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.333 1.08E-87 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.315 2.52E-78 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.305 2.25E-73 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.300 6.65E-71 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.285 7.64E-64 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.285 1.35E-63 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.278 2.24E-60 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.263 2.43E-54 YOR008C SLG1 hom Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.246 1.56E-47 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.246 2.16E-47 YML128C MSC1 hom Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
0.244 1.61E-46 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.239 7.93E-45 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.235 1.96E-43 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.228 1.09E-40 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.227 2.23E-40 YKL114C APN1 hom Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine