YLR376C / PSY3

Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR376C / PSY3

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:3.21|P-value:7.33E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:4.24|P-value:1.40E-5 ID:SGTC_248|Compound:streptozotocin|FD-Score:6.62|P-value:3.47E-11 ID:SGTC_266|Compound:formosanin C|FD-Score:4.10|P-value:2.56E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.42|P-value:3.49E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.89|P-value:5.99E-5 ID:SGTC_500|Compound:h-89|FD-Score:3.24|P-value:6.52E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:4.93|P-value:5.64E-7 ID:SGTC_532|Compound:1548-0774|FD-Score:3.77|P-value:9.43E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:3.71|P-value:1.20E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.36|P-value:4.40E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:4.43|P-value:5.88E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:3.76|P-value:9.86E-5 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:4.29|P-value:1.13E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.13|P-value:2.25E-5 ID:SGTC_975|Compound:1335-0046|FD-Score:4.56|P-value:3.25E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:4.22|P-value:1.49E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.31|P-value:6.49E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.05|P-value:3.13E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.53|P-value:2.38E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.90|P-value:5.62E-5 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.55|P-value:2.22E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.49|P-value:2.75E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:5.32|P-value:7.68E-8 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-4.14|P-value:2.88E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.39|P-value:3.89E-4 ID:SGTC_1742|Compound:st038235|FD-Score:-4.24|P-value:1.87E-5 ID:SGTC_1822|Compound:st052468|FD-Score:3.75|P-value:1.02E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.68|P-value:1.36E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.70|P-value:1.61E-4 ID:SGTC_2058|Compound:5236698|FD-Score:-3.87|P-value:8.33E-5 ID:SGTC_2102|Compound:5377816|FD-Score:-3.91|P-value:7.19E-5 ID:SGTC_2146|Compound:5545642|FD-Score:3.83|P-value:7.49E-5 ID:SGTC_2377|Compound:9073351|FD-Score:3.96|P-value:4.43E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.44|P-value:3.34E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.65|P-value:1.50E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:4.52|P-value:4.01E-6 ID:SGTC_2799|Compound:7812892|FD-Score:6.23|P-value:3.97E-10 ID:SGTC_2849|Compound:9019321|FD-Score:3.25|P-value:6.34E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.20|P-value:9.49E-4 ID:SGTC_2958|Compound:9077254|FD-Score:4.24|P-value:1.36E-5 ID:SGTC_3000|Compound:9075062|FD-Score:3.46|P-value:3.02E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.89|P-value:5.96E-5 ID:SGTC_3013|Compound:9080133|FD-Score:3.64|P-value:1.56E-4 ID:SGTC_3035|Compound:9091303|FD-Score:3.26|P-value:6.16E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_3213|Compound:9129884|FD-Score:4.35|P-value:8.68E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:3.21|P-value:7.33E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:4.24|P-value:1.40E-5 ID:SGTC_248|Compound:streptozotocin|FD-Score:6.62|P-value:3.47E-11 ID:SGTC_266|Compound:formosanin C|FD-Score:4.10|P-value:2.56E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.42|P-value:3.49E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.89|P-value:5.99E-5 ID:SGTC_500|Compound:h-89|FD-Score:3.24|P-value:6.52E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:4.93|P-value:5.64E-7 ID:SGTC_532|Compound:1548-0774|FD-Score:3.77|P-value:9.43E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:3.71|P-value:1.20E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.36|P-value:4.40E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:4.43|P-value:5.88E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:3.76|P-value:9.86E-5 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:4.29|P-value:1.13E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.13|P-value:2.25E-5 ID:SGTC_975|Compound:1335-0046|FD-Score:4.56|P-value:3.25E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:4.22|P-value:1.49E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.31|P-value:6.49E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.05|P-value:3.13E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.53|P-value:2.38E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.90|P-value:5.62E-5 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.55|P-value:2.22E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.49|P-value:2.75E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:5.32|P-value:7.68E-8 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-4.14|P-value:2.88E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.39|P-value:3.89E-4 ID:SGTC_1742|Compound:st038235|FD-Score:-4.24|P-value:1.87E-5 ID:SGTC_1822|Compound:st052468|FD-Score:3.75|P-value:1.02E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.68|P-value:1.36E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.70|P-value:1.61E-4 ID:SGTC_2058|Compound:5236698|FD-Score:-3.87|P-value:8.33E-5 ID:SGTC_2102|Compound:5377816|FD-Score:-3.91|P-value:7.19E-5 ID:SGTC_2146|Compound:5545642|FD-Score:3.83|P-value:7.49E-5 ID:SGTC_2377|Compound:9073351|FD-Score:3.96|P-value:4.43E-5 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.44|P-value:3.34E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.65|P-value:1.50E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:4.52|P-value:4.01E-6 ID:SGTC_2799|Compound:7812892|FD-Score:6.23|P-value:3.97E-10 ID:SGTC_2849|Compound:9019321|FD-Score:3.25|P-value:6.34E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.20|P-value:9.49E-4 ID:SGTC_2958|Compound:9077254|FD-Score:4.24|P-value:1.36E-5 ID:SGTC_3000|Compound:9075062|FD-Score:3.46|P-value:3.02E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.89|P-value:5.96E-5 ID:SGTC_3013|Compound:9080133|FD-Score:3.64|P-value:1.56E-4 ID:SGTC_3035|Compound:9091303|FD-Score:3.26|P-value:6.16E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_3213|Compound:9129884|FD-Score:4.35|P-value:8.68E-6

Top fitness defect scores for YLR376C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_248 streptozotocin 6.62 5.2 mM 3.47E-11 DNA damage response 10 1.84E-11
2 SGTC_2799 7812892 6.23 81.8 µM 3.97E-10 superoxide 8 2.29E-10
3 SGTC_1574 spiramycin 5.32 23.7 µM 7.68E-8 9 5.26E-8
4 SGTC_530 4358-1714 4.93 28.0 µM 5.64E-7 17 4.12E-7
5 SGTC_975 1335-0046 4.56 28.5 µM 3.25E-6 RPP1 & pyrimidine depletion 18 2.51E-6
6 SGTC_2642 cholecalciferol 4.52 100.0 µM 4.01E-6 16 3.11E-6
7 SGTC_829 1635-0120 4.43 117.0 µM 5.88E-6 DNA damage response 29 4.63E-6
8 SGTC_3213 9129884 4.35 49.5 µM 8.68E-6 16 6.91E-6
9 SGTC_905 2-aminofluorene 4.29 215.0 µM 1.13E-5 cell wall signaling 20 9.07E-6
10 SGTC_2958 9077254 4.24 16.7 µM 1.36E-5 22 1.10E-5
11 SGTC_93 0109-0045 4.24 59.2 µM 1.40E-5 DNA damage response 33 1.13E-5
12 SGTC_1014 4130-3005 4.22 69.3 µM 1.49E-5 11 1.20E-5
13 SGTC_915 methyl methanesulfonate (MMS) 4.13 110.0 mM 2.25E-5 DNA damage response 37 1.85E-5
14 SGTC_266 formosanin C 4.10 406.0 nM 2.56E-5 sphingolipid biosynthesis & PDR1 64 2.10E-5
15 SGTC_1028 k015-0029 4.05 4.8 µM 3.13E-5 24 2.59E-5
16 SGTC_2377 9073351 3.96 62.6 µM 4.43E-5 ergosterol biosynthesis 23 3.70E-5
17 SGTC_1464 k208-0027 3.90 110.0 µM 5.62E-5 26 4.73E-5
18 SGTC_3012 9080433 3.89 71.4 µM 5.96E-5 14 5.03E-5
19 SGTC_360 0957-0399 3.89 1.0 µM 5.99E-5 cell wall 45 5.05E-5
20 SGTC_2146 5545642 3.83 200.0 µM 7.49E-5 20 6.36E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 2.08E-27 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.177 5.98E-25 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.167 2.51E-22 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.152 9.99E-19 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.146 2.01E-17 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.143 7.74E-17 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.117 9.76E-12 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.105 9.18E-10 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.105 1.28E-9 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.103 2.19E-9 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.102 3.40E-9 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.101 4.18E-9 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.098 1.27E-8 YER142C MAG1 hom 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
0.097 1.87E-8 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.097 2.03E-8 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein