YLR377C / FBP1

Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR377C / FBP1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:-3.48|P-value:3.26E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:-3.27|P-value:6.72E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.58|P-value:2.22E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.91|P-value:6.39E-5 ID:SGTC_459|Compound:nigericin|FD-Score:3.30|P-value:6.02E-4 ID:SGTC_512|Compound:prima-1|FD-Score:-3.19|P-value:8.92E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.73|P-value:1.27E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:5.02|P-value:4.26E-7 ID:SGTC_860|Compound:0443-0016|FD-Score:3.23|P-value:7.76E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.74|P-value:1.23E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.38|P-value:4.65E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.19|P-value:8.64E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.39|P-value:4.39E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.87|P-value:7.31E-5 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.64|P-value:1.81E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:5.97|P-value:2.43E-9 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.23|P-value:7.77E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.50|P-value:3.02E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.25|P-value:7.11E-4 ID:SGTC_1636|Compound:st007707|FD-Score:4.13|P-value:2.58E-5 ID:SGTC_1768|Compound:st044301|FD-Score:3.98|P-value:4.73E-5 ID:SGTC_1847|Compound:st056231|FD-Score:3.94|P-value:5.57E-5 ID:SGTC_1861|Compound:st057647|FD-Score:3.64|P-value:1.80E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.51|P-value:4.79E-6 ID:SGTC_1952|Compound:st077036|FD-Score:-3.24|P-value:7.41E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.41|P-value:4.09E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.27|P-value:6.68E-4 ID:SGTC_2327|Compound:6189656|FD-Score:-4.92|P-value:6.91E-7 ID:SGTC_2397|Compound:5161689|FD-Score:-3.41|P-value:4.11E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.98|P-value:4.65E-5 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-4.14|P-value:2.48E-5 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.61|P-value:2.02E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.64|P-value:1.78E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.15|P-value:0.00100 ID:SGTC_2892|Compound:9047358|FD-Score:4.28|P-value:1.37E-5 ID:SGTC_3005|Compound:9076496|FD-Score:3.25|P-value:7.09E-4 ID:SGTC_3006|Compound:9075424|FD-Score:4.76|P-value:1.50E-6 ID:SGTC_3044|Compound:9093193|FD-Score:-3.34|P-value:5.34E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.63|P-value:1.82E-4 ID:SGTC_3106|Compound:9121286|FD-Score:-3.58|P-value:2.20E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-3.62|P-value:1.94E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.42|P-value:3.97E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.17|P-value:9.38E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.48|P-value:3.26E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:-3.27|P-value:6.72E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.58|P-value:2.22E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.91|P-value:6.39E-5 ID:SGTC_459|Compound:nigericin|FD-Score:3.30|P-value:6.02E-4 ID:SGTC_512|Compound:prima-1|FD-Score:-3.19|P-value:8.92E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.73|P-value:1.27E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:5.02|P-value:4.26E-7 ID:SGTC_860|Compound:0443-0016|FD-Score:3.23|P-value:7.76E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.74|P-value:1.23E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.38|P-value:4.65E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.19|P-value:8.64E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.39|P-value:4.39E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.87|P-value:7.31E-5 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.64|P-value:1.81E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:5.97|P-value:2.43E-9 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.23|P-value:7.77E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.50|P-value:3.02E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.25|P-value:7.11E-4 ID:SGTC_1636|Compound:st007707|FD-Score:4.13|P-value:2.58E-5 ID:SGTC_1768|Compound:st044301|FD-Score:3.98|P-value:4.73E-5 ID:SGTC_1847|Compound:st056231|FD-Score:3.94|P-value:5.57E-5 ID:SGTC_1861|Compound:st057647|FD-Score:3.64|P-value:1.80E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.51|P-value:4.79E-6 ID:SGTC_1952|Compound:st077036|FD-Score:-3.24|P-value:7.41E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.41|P-value:4.09E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.27|P-value:6.68E-4 ID:SGTC_2327|Compound:6189656|FD-Score:-4.92|P-value:6.91E-7 ID:SGTC_2397|Compound:5161689|FD-Score:-3.41|P-value:4.11E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.98|P-value:4.65E-5 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-4.14|P-value:2.48E-5 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.61|P-value:2.02E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.64|P-value:1.78E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.15|P-value:0.00100 ID:SGTC_2892|Compound:9047358|FD-Score:4.28|P-value:1.37E-5 ID:SGTC_3005|Compound:9076496|FD-Score:3.25|P-value:7.09E-4 ID:SGTC_3006|Compound:9075424|FD-Score:4.76|P-value:1.50E-6 ID:SGTC_3044|Compound:9093193|FD-Score:-3.34|P-value:5.34E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.63|P-value:1.82E-4 ID:SGTC_3106|Compound:9121286|FD-Score:-3.58|P-value:2.20E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-3.62|P-value:1.94E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.42|P-value:3.97E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.17|P-value:9.38E-4

Top fitness defect scores for YLR377C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1160 4092-0570 5.97 10.5 µM 2.43E-9 plasma membrane duress 15 1.22E-9
2 SGTC_783 0848-0141 5.02 92.7 µM 4.26E-7 RSC & ERG11 6 2.60E-7
3 SGTC_3006 9075424 4.76 71.4 µM 1.50E-6 3 9.58E-7
4 SGTC_1941 st076221 4.51 61.8 µM 4.79E-6 sphingolipid biosynthesis & PDR1 59 3.20E-6
5 SGTC_2892 9047358 4.28 58.4 µM 1.37E-5 5 9.53E-6
6 SGTC_1636 st007707 4.13 68.9 µM 2.58E-5 47 1.84E-5
7 SGTC_2499 avocadenofuran 3.98 51.5 µM 4.65E-5 34 3.39E-5
8 SGTC_1768 st044301 3.98 37.2 µM 4.73E-5 16 3.44E-5
9 SGTC_1847 st056231 3.94 15.0 µM 5.57E-5 37 4.08E-5
10 SGTC_338 4055-0136 3.91 114.0 µM 6.39E-5 11 4.71E-5
11 SGTC_1130 3454-2858 3.87 15.9 µM 7.31E-5 NEO1 92 5.41E-5
12 SGTC_923 1900-2434 3.74 73.3 µM 1.23E-4 RNA processing & uracil transport 37 9.31E-5
13 SGTC_582 0987-0078 3.73 156.0 µM 1.27E-4 calcium & mitochondrial duress 81 9.61E-5
14 SGTC_2322 7413319 3.72 38.2 µM 1.30E-4 ERAD & cell cycle 169 9.86E-5
15 SGTC_2610 ononetin 3.64 44.2 µM 1.78E-4 55 1.36E-4
16 SGTC_1861 st057647 3.64 17.4 µM 1.80E-4 29 1.38E-4
17 SGTC_1144 3486-0183 3.64 12.5 µM 1.81E-4 32 1.38E-4
18 SGTC_3054 9094397 3.63 49.5 µM 1.82E-4 fatty acid desaturase (OLE1) 44 1.40E-4
19 SGTC_2596 sinensetin 3.61 100.0 µM 2.02E-4 44 1.55E-4
20 SGTC_237 nikkomycin 3.58 200.0 µM 2.22E-4 RSC complex & mRNA processing 20 1.71E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.096 2.93E-8 YJR047C ANB1 hom Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication
0.093 7.00E-8 YMR283C RIT1 hom 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.093 7.57E-8 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.089 2.35E-7 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.088 3.35E-7 YLR354C TAL1 hom Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
0.087 3.88E-7 YGR225W AMA1 hom Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis
0.081 2.72E-6 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.080 3.84E-6 YNL022C_p YNL022C_p hom Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis
0.080 3.87E-6 YKL221W MCH2 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.079 4.08E-6 YDL098C SNU23 het Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
0.078 5.81E-6 YOR107W RGS2 hom Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
0.078 6.37E-6 YBR088C POL30 het Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
0.076 1.12E-5 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.076 1.14E-5 YOR356W CIR2 hom Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response
0.075 1.49E-5 YMR253C_p YMR253C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene