HIPHOP chemogenomics database

YLR382C / NAM2

Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR382C / NAM2

Click on Significant Values for Screen Details

Top fitness defect scores for YLR382C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	8.45	5.3 µM	8.52E-16		236	1.41E-17
2	SGTC_2849	9019321	8.43	13.0 µM	1.03E-15		4	1.75E-17
3	SGTC_386	1509-0011	7.83	3.1 µM	8.72E-14		66	2.53E-15
4	SGTC_1249	0481-0015	6.82	10.2 µM	7.01E-11		6	4.59E-12
5	SGTC_712	r015-0009	6.00	80.6 µM	8.49E-9		8	9.95E-10
6	SGTC_2802	7925815	5.73	54.5 µM	3.62E-8		9	5.06E-9
7	SGTC_365	0121-0052	5.30	13.6 µM	3.18E-7	mitochondrial response to ROS	23	5.79E-8
8	SGTC_800	3448-7362	5.16	155.0 µM	6.36E-7	translation	82	1.26E-7
9	SGTC_10	0279-0212	5.15	42.6 µM	6.50E-7	TRP & mitochondrial translation	55	1.29E-7
10	SGTC_2266	7929956	5.07	200.0 µM	9.75E-7		13	2.03E-7
11	SGTC_1742	st038235	4.89	28.9 µM	2.23E-6	mitochondrial stress	82	5.13E-7
12	SGTC_2854	9024559	4.76	71.4 µM	3.91E-6		10	9.66E-7
13	SGTC_2785	7756463	4.75	72.7 µM	4.05E-6		13	1.00E-6
14	SGTC_1182	1222-0449	4.72	220.0 µM	4.71E-6		6	1.19E-6
15	SGTC_70	0141-0289	4.56	43.3 µM	9.30E-6	mitochondrial stress	133	2.55E-6
16	SGTC_2783	7738165	4.51	71.4 µM	1.14E-5		19	3.19E-6
17	SGTC_157	1348-1695	4.44	65.8 µM	1.55E-5	iron homeostasis	46	4.50E-6
18	SGTC_1796	st049173	4.42	110.4 µM	1.68E-5		9	4.95E-6
19	SGTC_462	ag-879	4.38	158.0 µM	1.96E-5		18	5.86E-6
20	SGTC_882	0843-0131	4.32	351.0 µM	2.51E-5		11	7.76E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.303	4.75E-72	YHR116W	COX23	hom	Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.300	2.93E-70	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.296	1.07E-68	YDR237W	MRPL7	hom	Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
0.294	1.43E-67	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.293	2.72E-67	YJL022W_d	YJL022W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.293	3.31E-67	YPR166C	MRP2	hom	Mitochondrial ribosomal protein of the small subunit
0.281	6.11E-62	YNL213C	RRG9	hom	Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.278	1.78E-60	YOR158W	PET123	hom	Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
0.277	5.00E-60	YGR150C	CCM1	hom	Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
0.275	3.04E-59	YMR138W	CIN4	hom	GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
0.268	5.31E-56	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.263	6.28E-54	YNL252C	MRPL17	hom	Mitochondrial ribosomal protein of the large subunit
0.263	6.36E-54	YJL023C	PET130	hom	Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.260	1.40E-52	YJL062W-A	COA3	hom	Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly
0.258	6.68E-52	YJR144W	MGM101	hom	Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage