YLR384C / IKI3

Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR384C / IKI3

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.23|P-value:5.35E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.81|P-value:8.60E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.27|P-value:4.70E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.52|P-value:1.88E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.08|P-value:9.11E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.80|P-value:8.97E-5 ID:SGTC_252|Compound:miconazole|FD-Score:-3.83|P-value:7.79E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:5.52|P-value:1.35E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:3.52|P-value:1.82E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:-3.15|P-value:9.58E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:4.42|P-value:4.03E-6 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.15|P-value:9.76E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.49|P-value:2.08E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.60|P-value:1.38E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:5.81|P-value:2.43E-9 ID:SGTC_800|Compound:3448-7362|FD-Score:3.14|P-value:7.24E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:4.88|P-value:4.31E-7 ID:SGTC_940|Compound:3232-1287|FD-Score:-4.69|P-value:1.77E-6 ID:SGTC_941|Compound:3237-0776|FD-Score:4.18|P-value:1.20E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:3.78|P-value:6.73E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:4.01|P-value:2.53E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:3.30|P-value:4.23E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:4.12|P-value:1.61E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.65|P-value:1.59E-4 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.92|P-value:3.74E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.04|P-value:2.27E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.34|P-value:5.91E-6 ID:SGTC_1578|Compound:josamycin|FD-Score:3.10|P-value:8.44E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.65|P-value:1.11E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.94|P-value:4.97E-5 ID:SGTC_1827|Compound:berberine|FD-Score:4.27|P-value:8.21E-6 ID:SGTC_1842|Compound:st055362|FD-Score:-4.26|P-value:1.28E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:17.90|P-value:2.29E-72 ID:SGTC_1927|Compound:st060202|FD-Score:3.42|P-value:2.72E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.74|P-value:7.79E-5 ID:SGTC_2104|Compound:5389443|FD-Score:3.62|P-value:1.27E-4 ID:SGTC_2105|Compound:5350118|FD-Score:7.73|P-value:4.13E-15 ID:SGTC_2109|Compound:5483026|FD-Score:5.19|P-value:8.38E-8 ID:SGTC_2306|Compound:7482397|FD-Score:-3.41|P-value:3.94E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.24|P-value:7.26E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.07|P-value:9.28E-4 ID:SGTC_2387|Compound:9077560|FD-Score:3.08|P-value:8.85E-4 ID:SGTC_2427|Compound:5545055|FD-Score:3.13|P-value:7.47E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.66|P-value:1.77E-18 ID:SGTC_2679|Compound:rapamycin|FD-Score:9.89|P-value:1.68E-23 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.23|P-value:1.82E-10 ID:SGTC_2724|Compound:maprotiline|FD-Score:-3.91|P-value:5.63E-5 ID:SGTC_2730|Compound:imipramine|FD-Score:3.59|P-value:1.40E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.69|P-value:1.38E-4 ID:SGTC_2742|Compound:clothiapine|FD-Score:7.22|P-value:1.95E-13 ID:SGTC_2770|Compound:toltrazuril|FD-Score:6.30|P-value:1.19E-10 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:3.35|P-value:3.45E-4 ID:SGTC_2802|Compound:7925815|FD-Score:3.69|P-value:9.54E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.13|P-value:7.54E-4 ID:SGTC_3089|Compound:9117425|FD-Score:-3.45|P-value:3.35E-4 ID:SGTC_3095|Compound:9117207|FD-Score:3.68|P-value:1.01E-4 ID:SGTC_3098|Compound:9119039|FD-Score:-3.82|P-value:8.18E-5 ID:SGTC_3115|Compound:9123671|FD-Score:3.36|P-value:3.30E-4 ID:SGTC_3317|Compound:9138495|FD-Score:3.82|P-value:5.58E-5 ID:SGTC_3326|Compound:9140064|FD-Score:3.33|P-value:3.73E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.62|P-value:1.27E-4 ID:SGTC_3353|Compound:9151644|FD-Score:3.42|P-value:2.70E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.23|P-value:5.35E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.81|P-value:8.60E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.27|P-value:4.70E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.52|P-value:1.88E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.08|P-value:9.11E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.80|P-value:8.97E-5 ID:SGTC_252|Compound:miconazole|FD-Score:-3.83|P-value:7.79E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:5.52|P-value:1.35E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:3.52|P-value:1.82E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:-3.15|P-value:9.58E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:4.42|P-value:4.03E-6 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.15|P-value:9.76E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.49|P-value:2.08E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.60|P-value:1.38E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:5.81|P-value:2.43E-9 ID:SGTC_800|Compound:3448-7362|FD-Score:3.14|P-value:7.24E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:4.88|P-value:4.31E-7 ID:SGTC_940|Compound:3232-1287|FD-Score:-4.69|P-value:1.77E-6 ID:SGTC_941|Compound:3237-0776|FD-Score:4.18|P-value:1.20E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:3.78|P-value:6.73E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:4.01|P-value:2.53E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:3.30|P-value:4.23E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:4.12|P-value:1.61E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.65|P-value:1.59E-4 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.92|P-value:3.74E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.04|P-value:2.27E-5 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.34|P-value:5.91E-6 ID:SGTC_1578|Compound:josamycin|FD-Score:3.10|P-value:8.44E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.65|P-value:1.11E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.94|P-value:4.97E-5 ID:SGTC_1827|Compound:berberine|FD-Score:4.27|P-value:8.21E-6 ID:SGTC_1842|Compound:st055362|FD-Score:-4.26|P-value:1.28E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:17.90|P-value:2.29E-72 ID:SGTC_1927|Compound:st060202|FD-Score:3.42|P-value:2.72E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.74|P-value:7.79E-5 ID:SGTC_2104|Compound:5389443|FD-Score:3.62|P-value:1.27E-4 ID:SGTC_2105|Compound:5350118|FD-Score:7.73|P-value:4.13E-15 ID:SGTC_2109|Compound:5483026|FD-Score:5.19|P-value:8.38E-8 ID:SGTC_2306|Compound:7482397|FD-Score:-3.41|P-value:3.94E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.24|P-value:7.26E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.07|P-value:9.28E-4 ID:SGTC_2387|Compound:9077560|FD-Score:3.08|P-value:8.85E-4 ID:SGTC_2427|Compound:5545055|FD-Score:3.13|P-value:7.47E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.66|P-value:1.77E-18 ID:SGTC_2679|Compound:rapamycin|FD-Score:9.89|P-value:1.68E-23 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.23|P-value:1.82E-10 ID:SGTC_2724|Compound:maprotiline|FD-Score:-3.91|P-value:5.63E-5 ID:SGTC_2730|Compound:imipramine|FD-Score:3.59|P-value:1.40E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.69|P-value:1.38E-4 ID:SGTC_2742|Compound:clothiapine|FD-Score:7.22|P-value:1.95E-13 ID:SGTC_2770|Compound:toltrazuril|FD-Score:6.30|P-value:1.19E-10 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:3.35|P-value:3.45E-4 ID:SGTC_2802|Compound:7925815|FD-Score:3.69|P-value:9.54E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.13|P-value:7.54E-4 ID:SGTC_3089|Compound:9117425|FD-Score:-3.45|P-value:3.35E-4 ID:SGTC_3095|Compound:9117207|FD-Score:3.68|P-value:1.01E-4 ID:SGTC_3098|Compound:9119039|FD-Score:-3.82|P-value:8.18E-5 ID:SGTC_3115|Compound:9123671|FD-Score:3.36|P-value:3.30E-4 ID:SGTC_3317|Compound:9138495|FD-Score:3.82|P-value:5.58E-5 ID:SGTC_3326|Compound:9140064|FD-Score:3.33|P-value:3.73E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.62|P-value:1.27E-4 ID:SGTC_3353|Compound:9151644|FD-Score:3.42|P-value:2.70E-4

Top fitness defect scores for YLR384C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 17.90 4.0 nM 2.29E-72 8 3.25E-72
2 SGTC_2679 rapamycin 9.89 1.0 nM 1.68E-23 27 2.31E-23
3 SGTC_2582 agaric acid 8.66 100.0 µM 1.77E-18 redox potentiating 92 2.37E-18
4 SGTC_2105 5350118 7.73 200.0 µM 4.13E-15 2 5.43E-15
5 SGTC_2742 clothiapine 7.22 36.3 µM 1.95E-13 NEO1 2 2.54E-13
6 SGTC_2770 toltrazuril 6.30 17.5 µM 1.19E-10 5 1.51E-10
7 SGTC_2688 kanamycin b 6.23 96.7 µM 1.82E-10 calcium & mitochondrial duress 142 2.31E-10
8 SGTC_775 4577-0580 5.81 57.3 µM 2.43E-9 1 3.05E-9
9 SGTC_265 mycophenolic acid 5.52 32.9 µM 1.35E-8 RPP1 & pyrimidine depletion 2 1.68E-8
10 SGTC_2109 5483026 5.19 200.0 µM 8.38E-8 RSC complex & mRNA processing 9 1.03E-7
11 SGTC_860 0443-0016 4.88 25.1 µM 4.31E-7 14 5.26E-7
12 SGTC_629 3448-6466 4.42 76.7 µM 4.03E-6 9 4.85E-6
13 SGTC_1447 4239-0816 4.34 81.9 µM 5.91E-6 20 7.09E-6
14 SGTC_1827 berberine 4.27 7.2 µM 8.21E-6 60S ribosome export 15 9.84E-6
15 SGTC_941 3237-0776 4.18 460.0 µM 1.20E-5 11 1.43E-5
16 SGTC_1126 isoteolin 4.12 1.0 µM 1.61E-5 24 1.91E-5
17 SGTC_1365 1672-3266 4.04 55.4 µM 2.27E-5 16 2.69E-5
18 SGTC_952 1185-0122 4.01 232.0 µM 2.53E-5 17 3.00E-5
19 SGTC_1333 1482-0047 3.92 286.0 µM 3.74E-5 18 4.43E-5
20 SGTC_3317 9138495 3.82 8.8 µM 5.58E-5 35 6.59E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.395 1.45E-125 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.374 3.41E-112 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.372 1.90E-110 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.351 4.07E-98 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.334 3.95E-88 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.328 6.25E-85 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.312 1.55E-76 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.290 3.35E-66 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.283 6.55E-63 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.281 4.55E-62 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.280 1.98E-61 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.272 5.65E-58 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.271 3.06E-57 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.265 6.25E-55 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.254 1.61E-50 YPL102C_d YPL102C_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4