YLR386W / VAC14

Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR386W / VAC14

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:4.12|P-value:1.84E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.71|P-value:6.83E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:4.95|P-value:9.04E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.42|P-value:5.47E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:4.60|P-value:3.41E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:5.03|P-value:6.67E-6 ID:SGTC_197|Compound:4544-0043|FD-Score:4.24|P-value:1.24E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:3.88|P-value:4.01E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:5.18|P-value:3.63E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:6.00|P-value:9.97E-8 ID:SGTC_607|Compound:4491-0986|FD-Score:4.33|P-value:9.04E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:5.87|P-value:1.84E-7 ID:SGTC_699|Compound:0375-0592|FD-Score:4.99|P-value:7.89E-6 ID:SGTC_706|Compound:4262-0057|FD-Score:4.26|P-value:1.15E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:5.56|P-value:7.41E-7 ID:SGTC_954|Compound:1189-0853|FD-Score:3.77|P-value:5.54E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.79|P-value:5.23E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:6.94|P-value:8.72E-10 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.69|P-value:7.11E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:5.07|P-value:5.70E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.59|P-value:9.65E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.83|P-value:4.63E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.47|P-value:5.57E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.98|P-value:1.12E-7 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.99|P-value:2.81E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.84|P-value:4.51E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.68|P-value:7.27E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.66|P-value:2.78E-5 ID:SGTC_1522|Compound:st012842|FD-Score:3.66|P-value:7.86E-4 ID:SGTC_1691|Compound:st019933|FD-Score:-3.68|P-value:6.38E-4 ID:SGTC_1704|Compound:st028805|FD-Score:4.01|P-value:2.67E-4 ID:SGTC_1752|Compound:st044819|FD-Score:6.23|P-value:3.29E-8 ID:SGTC_1754|Compound:st042065|FD-Score:3.68|P-value:7.43E-4 ID:SGTC_1757|Compound:st041620|FD-Score:8.36|P-value:2.12E-13 ID:SGTC_1760|Compound:st045453|FD-Score:4.96|P-value:8.64E-6 ID:SGTC_1775|Compound:st081588|FD-Score:3.94|P-value:3.25E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.21|P-value:1.37E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.89|P-value:3.81E-4 ID:SGTC_1884|Compound:5633444|FD-Score:5.02|P-value:6.86E-6 ID:SGTC_1915|Compound:st067105|FD-Score:3.90|P-value:3.76E-4 ID:SGTC_1937|Compound:st074714|FD-Score:5.63|P-value:5.52E-7 ID:SGTC_1981|Compound:st071826|FD-Score:3.70|P-value:7.00E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.80|P-value:4.44E-4 ID:SGTC_2307|Compound:7538926|FD-Score:3.76|P-value:5.86E-4 ID:SGTC_2353|Compound:9059630|FD-Score:4.76|P-value:1.91E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.37|P-value:7.96E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:6.09|P-value:6.48E-8 ID:SGTC_2586|Compound:curcumin|FD-Score:4.17|P-value:1.57E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.81|P-value:1.57E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.71|P-value:6.74E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-3.54|P-value:9.74E-4 ID:SGTC_2953|Compound:9074162|FD-Score:-4.18|P-value:1.27E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.59|P-value:9.62E-4 ID:SGTC_3150|Compound:9097574|FD-Score:-4.12|P-value:1.57E-4 ID:SGTC_3153|Compound:9100258|FD-Score:-4.14|P-value:1.44E-4 ID:SGTC_3161|Compound:9100388|FD-Score:4.80|P-value:1.63E-5 ID:SGTC_3175|Compound:9106517|FD-Score:6.34|P-value:1.93E-8 ID:SGTC_3258|Compound:9138477|FD-Score:3.66|P-value:7.92E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.12|P-value:1.84E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.71|P-value:6.83E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:4.95|P-value:9.04E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.42|P-value:5.47E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:4.60|P-value:3.41E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:5.03|P-value:6.67E-6 ID:SGTC_197|Compound:4544-0043|FD-Score:4.24|P-value:1.24E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:3.88|P-value:4.01E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:5.18|P-value:3.63E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:6.00|P-value:9.97E-8 ID:SGTC_607|Compound:4491-0986|FD-Score:4.33|P-value:9.04E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:5.87|P-value:1.84E-7 ID:SGTC_699|Compound:0375-0592|FD-Score:4.99|P-value:7.89E-6 ID:SGTC_706|Compound:4262-0057|FD-Score:4.26|P-value:1.15E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:5.56|P-value:7.41E-7 ID:SGTC_954|Compound:1189-0853|FD-Score:3.77|P-value:5.54E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.79|P-value:5.23E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:6.94|P-value:8.72E-10 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.69|P-value:7.11E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:5.07|P-value:5.70E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.59|P-value:9.65E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.83|P-value:4.63E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.47|P-value:5.57E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.98|P-value:1.12E-7 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.99|P-value:2.81E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.84|P-value:4.51E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.68|P-value:7.27E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.66|P-value:2.78E-5 ID:SGTC_1522|Compound:st012842|FD-Score:3.66|P-value:7.86E-4 ID:SGTC_1691|Compound:st019933|FD-Score:-3.68|P-value:6.38E-4 ID:SGTC_1704|Compound:st028805|FD-Score:4.01|P-value:2.67E-4 ID:SGTC_1752|Compound:st044819|FD-Score:6.23|P-value:3.29E-8 ID:SGTC_1754|Compound:st042065|FD-Score:3.68|P-value:7.43E-4 ID:SGTC_1757|Compound:st041620|FD-Score:8.36|P-value:2.12E-13 ID:SGTC_1760|Compound:st045453|FD-Score:4.96|P-value:8.64E-6 ID:SGTC_1775|Compound:st081588|FD-Score:3.94|P-value:3.25E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.21|P-value:1.37E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.89|P-value:3.81E-4 ID:SGTC_1884|Compound:5633444|FD-Score:5.02|P-value:6.86E-6 ID:SGTC_1915|Compound:st067105|FD-Score:3.90|P-value:3.76E-4 ID:SGTC_1937|Compound:st074714|FD-Score:5.63|P-value:5.52E-7 ID:SGTC_1981|Compound:st071826|FD-Score:3.70|P-value:7.00E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.80|P-value:4.44E-4 ID:SGTC_2307|Compound:7538926|FD-Score:3.76|P-value:5.86E-4 ID:SGTC_2353|Compound:9059630|FD-Score:4.76|P-value:1.91E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.37|P-value:7.96E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:6.09|P-value:6.48E-8 ID:SGTC_2586|Compound:curcumin|FD-Score:4.17|P-value:1.57E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.81|P-value:1.57E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.71|P-value:6.74E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-3.54|P-value:9.74E-4 ID:SGTC_2953|Compound:9074162|FD-Score:-4.18|P-value:1.27E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.59|P-value:9.62E-4 ID:SGTC_3150|Compound:9097574|FD-Score:-4.12|P-value:1.57E-4 ID:SGTC_3153|Compound:9100258|FD-Score:-4.14|P-value:1.44E-4 ID:SGTC_3161|Compound:9100388|FD-Score:4.80|P-value:1.63E-5 ID:SGTC_3175|Compound:9106517|FD-Score:6.34|P-value:1.93E-8 ID:SGTC_3258|Compound:9138477|FD-Score:3.66|P-value:7.92E-4

Top fitness defect scores for YLR386W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1757 st041620 8.36 31.8 µM 2.12E-13 5 3.25E-17
2 SGTC_1005 4092-0690 6.94 4.4 µM 8.72E-10 plasma membrane duress 8 1.89E-12
3 SGTC_3175 9106517 6.34 49.5 µM 1.93E-8 iron homeostasis 2 1.12E-10
4 SGTC_1752 st044819 6.23 83.0 µM 3.29E-8 8 2.27E-10
5 SGTC_2552 juarezic acid 6.09 87.0 µM 6.48E-8 TSC3-RPN4 5 5.52E-10
6 SGTC_525 k781-2675 6.00 26.7 µM 9.97E-8 redox potentiating 25 9.74E-10
7 SGTC_1308 1187-1617 5.98 41.4 µM 1.12E-7 fatty acid desaturase (OLE1) 10 1.13E-9
8 SGTC_612 0302-0167 5.87 22.4 µM 1.84E-7 copper-dependent oxidative stress 8 2.18E-9
9 SGTC_1937 st074714 5.63 47.2 µM 5.52E-7 16 9.27E-9
10 SGTC_722 3534-0461 5.56 190.0 µM 7.41E-7 PDR1 11 1.36E-8
11 SGTC_208 k072-0098 5.18 12.4 µM 3.63E-6 heme biosynthesis & mitochondrial translocase 23 1.10E-7
12 SGTC_1066 ketoconazole 5.07 628.0 nM 5.70E-6 6 1.99E-7
13 SGTC_173 1273-0061 5.03 2.7 µM 6.67E-6 mitochondrial stress 147 2.45E-7
14 SGTC_1884 5633444 5.02 25.0 µM 6.86E-6 redox potentiating 32 2.54E-7
15 SGTC_699 0375-0592 4.99 111.0 µM 7.89E-6 iron homeostasis 8 3.05E-7
16 SGTC_1760 st045453 4.96 10.4 µM 8.64E-6 NEO1-PIK1 111 3.44E-7
17 SGTC_160 3453-0671 4.95 289.7 µM 9.04E-6 21 3.65E-7
18 SGTC_2806 7952793 4.81 58.4 µM 1.57E-5 9 7.52E-7
19 SGTC_3161 9100388 4.80 49.5 µM 1.63E-5 iron homeostasis 3 7.93E-7
20 SGTC_2353 9059630 4.76 146.7 µM 1.91E-5 8 9.73E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 5.42E-18 YJL204C RCY1 hom F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.145 2.45E-17 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.135 4.28E-15 YHL027W RIM101 hom Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
0.130 5.08E-14 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.125 3.88E-13 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.124 5.88E-13 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.123 7.41E-13 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.121 1.95E-12 YDL226C GCS1 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.118 6.79E-12 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.116 1.69E-11 YCR094W CDC50 hom Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
0.113 5.97E-11 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.111 1.26E-10 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.110 1.84E-10 YMR077C VPS20 hom Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.108 3.27E-10 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.106 8.02E-10 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc