HIPHOP chemogenomics database
YLR397C / AFG2 
ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YLR397C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.125 | 3.83E-13 | YDR416W | SYF1 | het | Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans |
| 0.119 | 5.45E-12 | YLR106C | MDN1 | het | Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus |
| 0.105 | 1.14E-9 | YDR460W | TFB3 | het | Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit |
| 0.102 | 2.74E-9 | YNL245C | CWC25 | het | Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p |
| 0.095 | 4.05E-8 | YKL088W | CAB3 | het | Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC |
| 0.094 | 5.15E-8 | YLR193C | UPS1 | hom | Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI |
| 0.089 | 2.80E-7 | YFR052W | RPN12 | het | Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress |
| 0.087 | 4.61E-7 | YBL071W-A | KTI11 | hom | Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs |
| 0.087 | 4.95E-7 | YBL041W | PRE7 | het | Beta 6 subunit of the 20S proteasome |
| 0.087 | 5.09E-7 | YER148W | SPT15 | het | TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability |
| 0.086 | 5.72E-7 | YCR071C | IMG2 | hom | Mitochondrial ribosomal protein of the large subunit |
| 0.082 | 1.99E-6 | YOR335C | ALA1 | het | Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog |
| 0.081 | 2.83E-6 | YJR017C | ESS1 | het | Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 |
| 0.080 | 3.16E-6 | YJL143W | TIM17 | het | Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex |
| 0.077 | 8.62E-6 | YLR418C | CDC73 | hom | Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress |