YLR397C / AFG2

ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR397C / AFG2

Click on Significant Values for Screen Details ID:SGTC_70|Compound:0141-0289|FD-Score:2.84|P-value:8.16E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-2.98|P-value:4.62E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:2.90|P-value:6.40E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:2.88|P-value:6.86E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.39|P-value:8.17E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:3.26|P-value:1.45E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.66|P-value:2.37E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-8.22|P-value:3.28E-20 ID:SGTC_496|Compound:calpeptin|FD-Score:3.28|P-value:1.33E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:-3.12|P-value:2.57E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.21|P-value:1.81E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.01|P-value:4.09E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:2.87|P-value:7.14E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.79|P-value:1.30E-5 ID:SGTC_657|Compound:3534-0529|FD-Score:3.14|P-value:2.46E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:-3.47|P-value:5.63E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:3.16|P-value:2.20E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.26|P-value:1.47E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.09|P-value:2.96E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:4.56|P-value:1.99E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.33|P-value:1.08E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:-2.90|P-value:6.32E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.25|P-value:1.56E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.44|P-value:6.67E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-9.45|P-value:4.06E-26 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.38|P-value:8.75E-5 ID:SGTC_1467|Compound:k284-1082|FD-Score:-2.83|P-value:8.33E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.73|P-value:1.67E-5 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.08|P-value:3.06E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.04|P-value:3.67E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.01|P-value:4.19E-4 ID:SGTC_1628|Compound:st004252|FD-Score:-4.05|P-value:3.42E-6 ID:SGTC_1636|Compound:st007707|FD-Score:-4.79|P-value:4.93E-8 ID:SGTC_1748|Compound:st037455|FD-Score:3.98|P-value:4.98E-6 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.54|P-value:2.27E-7 ID:SGTC_1814|Compound:st052110|FD-Score:3.40|P-value:8.04E-5 ID:SGTC_1980|Compound:st071811|FD-Score:3.92|P-value:6.52E-6 ID:SGTC_2003|Compound:4023581|FD-Score:2.83|P-value:8.18E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.04|P-value:3.63E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.00|P-value:4.29E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.05|P-value:3.48E-4 ID:SGTC_2215|Compound:7188065|FD-Score:3.68|P-value:2.14E-5 ID:SGTC_2287|Compound:7945887|FD-Score:-3.11|P-value:2.72E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.02|P-value:3.97E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.09|P-value:2.93E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.21|P-value:1.83E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.41|P-value:7.66E-5 ID:SGTC_2467|Compound:5474540|FD-Score:-2.82|P-value:8.70E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.19|P-value:1.98E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.14|P-value:2.43E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.82|P-value:1.12E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.00|P-value:4.25E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:3.23|P-value:1.70E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.21|P-value:1.80E-4 ID:SGTC_2885|Compound:9057732|FD-Score:-4.74|P-value:6.86E-8 ID:SGTC_2895|Compound:9054447|FD-Score:4.68|P-value:9.70E-8 ID:SGTC_2950|Compound:9072895|FD-Score:3.99|P-value:4.68E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-4.56|P-value:2.05E-7 ID:SGTC_2967|Compound:9089538|FD-Score:5.59|P-value:2.66E-10 ID:SGTC_3004|Compound:9076122|FD-Score:-2.91|P-value:6.07E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.03|P-value:3.87E-4 ID:SGTC_3210|Compound:9128157|FD-Score:2.83|P-value:8.24E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.15|P-value:2.36E-4 ID:SGTC_3260|Compound:9137109|FD-Score:3.43|P-value:6.95E-5 ID:SGTC_3319|Compound:9138450|FD-Score:2.80|P-value:9.47E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.14|P-value:2.46E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:2.84|P-value:8.16E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-2.98|P-value:4.62E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:2.90|P-value:6.40E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:2.88|P-value:6.86E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.39|P-value:8.17E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:3.26|P-value:1.45E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.66|P-value:2.37E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-8.22|P-value:3.28E-20 ID:SGTC_496|Compound:calpeptin|FD-Score:3.28|P-value:1.33E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:-3.12|P-value:2.57E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.21|P-value:1.81E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.01|P-value:4.09E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:2.87|P-value:7.14E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.79|P-value:1.30E-5 ID:SGTC_657|Compound:3534-0529|FD-Score:3.14|P-value:2.46E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:-3.47|P-value:5.63E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:3.16|P-value:2.20E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.26|P-value:1.47E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.09|P-value:2.96E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:4.56|P-value:1.99E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.33|P-value:1.08E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:-2.90|P-value:6.32E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.25|P-value:1.56E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.44|P-value:6.67E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-9.45|P-value:4.06E-26 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.38|P-value:8.75E-5 ID:SGTC_1467|Compound:k284-1082|FD-Score:-2.83|P-value:8.33E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.73|P-value:1.67E-5 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.08|P-value:3.06E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.04|P-value:3.67E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.01|P-value:4.19E-4 ID:SGTC_1628|Compound:st004252|FD-Score:-4.05|P-value:3.42E-6 ID:SGTC_1636|Compound:st007707|FD-Score:-4.79|P-value:4.93E-8 ID:SGTC_1748|Compound:st037455|FD-Score:3.98|P-value:4.98E-6 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.54|P-value:2.27E-7 ID:SGTC_1814|Compound:st052110|FD-Score:3.40|P-value:8.04E-5 ID:SGTC_1980|Compound:st071811|FD-Score:3.92|P-value:6.52E-6 ID:SGTC_2003|Compound:4023581|FD-Score:2.83|P-value:8.18E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.04|P-value:3.63E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.00|P-value:4.29E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.05|P-value:3.48E-4 ID:SGTC_2215|Compound:7188065|FD-Score:3.68|P-value:2.14E-5 ID:SGTC_2287|Compound:7945887|FD-Score:-3.11|P-value:2.72E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.02|P-value:3.97E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.09|P-value:2.93E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.21|P-value:1.83E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.41|P-value:7.66E-5 ID:SGTC_2467|Compound:5474540|FD-Score:-2.82|P-value:8.70E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.19|P-value:1.98E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.14|P-value:2.43E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.82|P-value:1.12E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.00|P-value:4.25E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:3.23|P-value:1.70E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.21|P-value:1.80E-4 ID:SGTC_2885|Compound:9057732|FD-Score:-4.74|P-value:6.86E-8 ID:SGTC_2895|Compound:9054447|FD-Score:4.68|P-value:9.70E-8 ID:SGTC_2950|Compound:9072895|FD-Score:3.99|P-value:4.68E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-4.56|P-value:2.05E-7 ID:SGTC_2967|Compound:9089538|FD-Score:5.59|P-value:2.66E-10 ID:SGTC_3004|Compound:9076122|FD-Score:-2.91|P-value:6.07E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.03|P-value:3.87E-4 ID:SGTC_3210|Compound:9128157|FD-Score:2.83|P-value:8.24E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.15|P-value:2.36E-4 ID:SGTC_3260|Compound:9137109|FD-Score:3.43|P-value:6.95E-5 ID:SGTC_3319|Compound:9138450|FD-Score:2.80|P-value:9.47E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.14|P-value:2.46E-4

Top fitness defect scores for YLR397C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2967 9089538 5.59 49.9 µM 2.66E-10 3 1.14E-8 0.67
2 SGTC_2895 9054447 4.68 58.4 µM 9.70E-8 1 1.40E-6 1.13
3 SGTC_938 3126-1755 4.56 342.0 µM 1.99E-7 1 2.52E-6 0.51
4 SGTC_1787 fluconazole 4.54 80.0 µM 2.27E-7 iron homeostasis 7 2.81E-6 0.11
5 SGTC_2950 9072895 3.99 21.5 µM 4.68E-6 10 3.32E-5 0.49
6 SGTC_1748 st037455 3.98 53.6 µM 4.98E-6 8 3.49E-5 0.09
7 SGTC_1980 st071811 3.92 45.7 µM 6.52E-6 RPP1 & pyrimidine depletion 3 4.36E-5 0.03
8 SGTC_2593 nobiletin 3.82 86.2 µM 1.12E-5 13 6.78E-5 0.20
9 SGTC_618 k072-0232 3.79 33.2 µM 1.30E-5 60S ribosome export 7 7.65E-5 0.05
10 SGTC_1501 4491-0275 3.73 38.4 µM 1.67E-5 4 9.41E-5 0.23
11 SGTC_2215 7188065 3.68 200.0 µM 2.14E-5 3 1.15E-4 0.45
12 SGTC_1237 0330-0133 3.44 46.1 µM 6.67E-5 ergosterol biosynthesis 15 2.92E-4 0.11
13 SGTC_3260 9137109 3.43 49.5 µM 6.95E-5 60S ribosome export 5 3.02E-4 0.05
14 SGTC_2454 5-benzylidenerhodanine 3.41 10.4 µM 7.66E-5 TRP & mitochondrial translation 11 3.28E-4 0.29
15 SGTC_1814 st052110 3.40 31.5 µM 8.04E-5 RNA processing & uracil transport 15 3.41E-4 0.01
16 SGTC_239 5hr heat shock (37°C) + mitomycin C 3.39 819.4 µM 8.17E-5 heat shock/prefoldin 4 3.45E-4 0.16
17 SGTC_1206 3978-0017 3.33 132.0 µM 1.08E-4 ERG2 13 4.35E-4 0.08
18 SGTC_496 calpeptin 3.28 138.0 µM 1.33E-4 12 5.15E-4 0.04
19 SGTC_375 3970-0961 3.26 9.3 µM 1.45E-4 tubulin folding & SWR complex 17 5.53E-4 0.05
20 SGTC_891 0929-0073 3.26 133.0 µM 1.47E-4 4 5.58E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 3.83E-13 YDR416W SYF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.119 5.45E-12 YLR106C MDN1 het Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
0.105 1.14E-9 YDR460W TFB3 het Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
0.102 2.74E-9 YNL245C CWC25 het Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
0.095 4.05E-8 YKL088W CAB3 het Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
0.094 5.15E-8 YLR193C UPS1 hom Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI
0.089 2.80E-7 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.087 4.61E-7 YBL071W-A KTI11 hom Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
0.087 4.95E-7 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.087 5.09E-7 YER148W SPT15 het TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
0.086 5.72E-7 YCR071C IMG2 hom Mitochondrial ribosomal protein of the large subunit
0.082 1.99E-6 YOR335C ALA1 het Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
0.081 2.83E-6 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.080 3.16E-6 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.077 8.62E-6 YLR418C CDC73 hom Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress