YLR400W_d

Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR400W / YLR400W

Click on Significant Values for Screen Details ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-3.03|P-value:8.65E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.17|P-value:5.30E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:-3.03|P-value:8.88E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.54|P-value:1.44E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.59|P-value:1.18E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.89|P-value:3.01E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.38|P-value:2.41E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.02|P-value:2.77E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.79|P-value:3.99E-7 ID:SGTC_1725|Compound:st036284|FD-Score:-3.43|P-value:2.02E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-3.71|P-value:6.51E-5 ID:SGTC_1765|Compound:st044815|FD-Score:3.15|P-value:6.21E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.31|P-value:3.47E-4 ID:SGTC_1865|Compound:st057639|FD-Score:-3.00|P-value:9.81E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:-3.48|P-value:1.66E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.50|P-value:1.69E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.43|P-value:1.99E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.17|P-value:5.79E-4 ID:SGTC_2123|Compound:5259487|FD-Score:-3.01|P-value:9.26E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.38|P-value:2.69E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.20|P-value:5.32E-4 ID:SGTC_2273|Compound:7815444|FD-Score:4.26|P-value:6.23E-6 ID:SGTC_2304|Compound:7443016|FD-Score:-3.20|P-value:4.72E-4 ID:SGTC_2312|Compound:7706453|FD-Score:3.09|P-value:7.94E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:4.25|P-value:6.56E-6 ID:SGTC_2873|Compound:9040596|FD-Score:-3.45|P-value:1.85E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.48|P-value:1.80E-4 ID:SGTC_3120|Compound:9124077|FD-Score:-3.22|P-value:4.37E-4 ID:SGTC_3122|Compound:9126309|FD-Score:-3.60|P-value:1.02E-4 ID:SGTC_3167|Compound:9103034|FD-Score:3.09|P-value:7.77E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.26|P-value:4.29E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.08|P-value:8.19E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.69|P-value:7.88E-5 ID:SGTC_3351|Compound:9150312|FD-Score:-3.02|P-value:9.08E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-3.03|P-value:8.65E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.17|P-value:5.30E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:-3.03|P-value:8.88E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.54|P-value:1.44E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.59|P-value:1.18E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.89|P-value:3.01E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.38|P-value:2.41E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.02|P-value:2.77E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.79|P-value:3.99E-7 ID:SGTC_1725|Compound:st036284|FD-Score:-3.43|P-value:2.02E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-3.71|P-value:6.51E-5 ID:SGTC_1765|Compound:st044815|FD-Score:3.15|P-value:6.21E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.31|P-value:3.47E-4 ID:SGTC_1865|Compound:st057639|FD-Score:-3.00|P-value:9.81E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:-3.48|P-value:1.66E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.50|P-value:1.69E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.43|P-value:1.99E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.17|P-value:5.79E-4 ID:SGTC_2123|Compound:5259487|FD-Score:-3.01|P-value:9.26E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.38|P-value:2.69E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.20|P-value:5.32E-4 ID:SGTC_2273|Compound:7815444|FD-Score:4.26|P-value:6.23E-6 ID:SGTC_2304|Compound:7443016|FD-Score:-3.20|P-value:4.72E-4 ID:SGTC_2312|Compound:7706453|FD-Score:3.09|P-value:7.94E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:4.25|P-value:6.56E-6 ID:SGTC_2873|Compound:9040596|FD-Score:-3.45|P-value:1.85E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.48|P-value:1.80E-4 ID:SGTC_3120|Compound:9124077|FD-Score:-3.22|P-value:4.37E-4 ID:SGTC_3122|Compound:9126309|FD-Score:-3.60|P-value:1.02E-4 ID:SGTC_3167|Compound:9103034|FD-Score:3.09|P-value:7.77E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.26|P-value:4.29E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.08|P-value:8.19E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.69|P-value:7.88E-5 ID:SGTC_3351|Compound:9150312|FD-Score:-3.02|P-value:9.08E-4

Top fitness defect scores for YLR400W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2273 7815444 4.26 169.1 µM 6.23E-6 16 1.01E-5
2 SGTC_2524 avocatin b 4.25 10.6 µM 6.56E-6 21 1.06E-5
3 SGTC_3251 9135047 3.69 49.5 µM 7.88E-5 60S ribosome export 6 1.12E-4
4 SGTC_770 0389-1121 3.59 67.2 µM 1.18E-4 RPP1 & pyrimidine depletion 39 1.65E-4
5 SGTC_480 am 92016 3.54 50.0 µM 1.44E-4 22 1.99E-4
6 SGTC_1874 staurosporine 3.50 7.6 µM 1.69E-4 cell wall signaling 42 2.33E-4
7 SGTC_2973 9094665 3.48 18.0 µM 1.80E-4 NEO1 44 2.47E-4
8 SGTC_2139 5578692 3.38 56.5 µM 2.69E-4 superoxide 29 3.62E-4
9 SGTC_1820 st053765 3.31 18.6 µM 3.47E-4 RPP1 & pyrimidine depletion 37 4.62E-4
10 SGTC_3234 9132280 3.26 49.5 µM 4.29E-4 44 5.65E-4
11 SGTC_2151 5563267 3.20 85.4 µM 5.32E-4 45 6.93E-4
12 SGTC_2113 5491891 3.17 107.0 µM 5.79E-4 TSC3-RPN4 60 7.52E-4
13 SGTC_1765 st044815 3.15 90.4 µM 6.21E-4 54 8.04E-4
14 SGTC_3167 9103034 3.09 49.5 µM 7.77E-4 RPP1 & pyrimidine depletion 18 9.95E-4
15 SGTC_2312 7706453 3.09 155.7 µM 7.94E-4 66 0.00102
16 SGTC_3238 9133470 3.08 49.5 µM 8.19E-4 36 0.00105
17 SGTC_2678 equilin 2.99 100.0 µM 0.00110 59 0.00138
18 SGTC_2518 prenyletin 2.98 46.3 µM 0.00115 33 0.00144
19 SGTC_897 1000-0139 2.98 31.4 µM 0.00116 55 0.00145
20 SGTC_1282 0886-0010 2.97 32.4 µM 0.00121 endomembrane recycling 77 0.00151

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.276 8.09E-60 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.274 2.60E-58 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.273 1.51E-58 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.261 2.22E-53 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.249 1.84E-48 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.238 2.14E-44 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.230 1.23E-41 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.220 5.47E-38 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.216 8.86E-37 YNL339C YRF1-6 hom Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.215 3.07E-36 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.212 2.08E-35 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.210 7.72E-35 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.210 1.27E-34 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.203 1.84E-32 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.203 1.44E-32 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C