YLR405W / DUS4

Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR405W / DUS4

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-8.23|P-value:5.01E-18 ID:SGTC_427|Compound:1354-0053|FD-Score:3.56|P-value:9.89E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.60|P-value:9.03E-5 ID:SGTC_480|Compound:am 92016|FD-Score:3.23|P-value:3.72E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:3.25|P-value:3.42E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:-4.11|P-value:9.25E-6 ID:SGTC_589|Compound:1227-0070|FD-Score:3.63|P-value:7.60E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:2.99|P-value:8.88E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.49|P-value:1.33E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.20|P-value:4.32E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-4.23|P-value:5.45E-6 ID:SGTC_1610|Compound:st001799|FD-Score:-3.45|P-value:1.63E-4 ID:SGTC_1911|Compound:st060208|FD-Score:3.43|P-value:1.67E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.27|P-value:3.22E-4 ID:SGTC_1970|Compound:st070122|FD-Score:-3.23|P-value:3.83E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.39|P-value:2.01E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.03|P-value:8.16E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-4.45|P-value:1.81E-6 ID:SGTC_2331|Compound:7980721|FD-Score:-3.44|P-value:1.71E-4 ID:SGTC_2534|Compound:helenine|FD-Score:2.97|P-value:9.48E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.58|P-value:9.00E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.30|P-value:2.92E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.07|P-value:6.92E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.16|P-value:4.80E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.34|P-value:2.58E-4 ID:SGTC_3128|Compound:9127691|FD-Score:-3.42|P-value:1.85E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-3.01|P-value:8.67E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-4.79|P-value:3.10E-7 ID:SGTC_3172|Compound:9105863|FD-Score:3.59|P-value:8.94E-5 ID:SGTC_3196|Compound:9111891|FD-Score:3.74|P-value:4.68E-5 ID:SGTC_3222|Compound:9128301|FD-Score:2.99|P-value:8.93E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-3.52|P-value:1.27E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.35|P-value:2.35E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.99|P-value:1.51E-5 ID:SGTC_3322|Compound:9139058|FD-Score:-3.79|P-value:4.07E-5 ID:SGTC_3330|Compound:9141424|FD-Score:-3.49|P-value:1.40E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-8.23|P-value:5.01E-18 ID:SGTC_427|Compound:1354-0053|FD-Score:3.56|P-value:9.89E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.60|P-value:9.03E-5 ID:SGTC_480|Compound:am 92016|FD-Score:3.23|P-value:3.72E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:3.25|P-value:3.42E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:-4.11|P-value:9.25E-6 ID:SGTC_589|Compound:1227-0070|FD-Score:3.63|P-value:7.60E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:2.99|P-value:8.88E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.49|P-value:1.33E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.20|P-value:4.32E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-4.23|P-value:5.45E-6 ID:SGTC_1610|Compound:st001799|FD-Score:-3.45|P-value:1.63E-4 ID:SGTC_1911|Compound:st060208|FD-Score:3.43|P-value:1.67E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.27|P-value:3.22E-4 ID:SGTC_1970|Compound:st070122|FD-Score:-3.23|P-value:3.83E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.39|P-value:2.01E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.03|P-value:8.16E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-4.45|P-value:1.81E-6 ID:SGTC_2331|Compound:7980721|FD-Score:-3.44|P-value:1.71E-4 ID:SGTC_2534|Compound:helenine|FD-Score:2.97|P-value:9.48E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.58|P-value:9.00E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-3.30|P-value:2.92E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.07|P-value:6.92E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.16|P-value:4.80E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.34|P-value:2.58E-4 ID:SGTC_3128|Compound:9127691|FD-Score:-3.42|P-value:1.85E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-3.01|P-value:8.67E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-4.79|P-value:3.10E-7 ID:SGTC_3172|Compound:9105863|FD-Score:3.59|P-value:8.94E-5 ID:SGTC_3196|Compound:9111891|FD-Score:3.74|P-value:4.68E-5 ID:SGTC_3222|Compound:9128301|FD-Score:2.99|P-value:8.93E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-3.52|P-value:1.27E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.35|P-value:2.35E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.99|P-value:1.51E-5 ID:SGTC_3322|Compound:9139058|FD-Score:-3.79|P-value:4.07E-5 ID:SGTC_3330|Compound:9141424|FD-Score:-3.49|P-value:1.40E-4

Top fitness defect scores for YLR405W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3316 9136639 3.99 10.6 µM 1.51E-5 12 3.25E-5
2 SGTC_3196 9111891 3.74 49.5 µM 4.68E-5 mitochondrial processes 25 9.23E-5
3 SGTC_589 1227-0070 3.63 34.1 µM 7.60E-5 38 1.44E-4
4 SGTC_3172 9105863 3.59 49.5 µM 8.94E-5 ERG2 9 1.68E-4
5 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 3.58 100.0 µM 9.00E-5 33 1.69E-4
6 SGTC_427 1354-0053 3.56 47.3 µM 9.89E-5 34 1.84E-4
7 SGTC_963 1269-2853 3.49 151.0 µM 1.33E-4 40 2.42E-4
8 SGTC_1911 st060208 3.43 74.3 µM 1.67E-4 52 2.98E-4
9 SGTC_2036 5160441 3.39 105.0 µM 2.01E-4 NEO1 102 3.55E-4
10 SGTC_3313 9133968 3.35 57.0 µM 2.35E-4 16 4.08E-4
11 SGTC_1916 st067606 3.27 49.2 µM 3.22E-4 73 5.47E-4
12 SGTC_521 r062-0005 3.25 13.8 µM 3.42E-4 37 5.77E-4
13 SGTC_480 am 92016 3.23 50.0 µM 3.72E-4 39 6.24E-4
14 SGTC_2922 7999257 3.16 32.5 µM 4.80E-4 30 7.91E-4
15 SGTC_597 k018-0003 2.99 82.8 µM 8.88E-4 RNA pol III & RNase P/MRP 43 0.00139
16 SGTC_3222 9128301 2.99 49.5 µM 8.93E-4 60S ribosome export 64 0.00140
17 SGTC_2534 helenine 2.97 20.1 µM 9.48E-4 RSC complex & mRNA processing 42 0.00148
18 SGTC_1412 3909-8563 2.93 178.0 µM 0.00112 53 0.00172
19 SGTC_591 4299-0174 2.91 154.0 µM 0.00119 41 0.00182
20 SGTC_3000 9075062 2.89 71.4 µM 0.00126 70 0.00193

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.229 4.18E-41 YLR414C PUN1 hom Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
0.190 1.70E-28 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.170 2.95E-23 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.157 4.86E-20 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.155 1.64E-19 YAL005C SSA1 hom ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
0.151 1.30E-18 YOR252W TMA16 hom Protein of unknown function that associates with ribosomes
0.148 6.34E-18 YLR263W RED1 hom Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
0.142 1.77E-16 YGR139W_d YGR139W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.138 1.04E-15 YBR222C PCS60 hom Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase
0.138 1.37E-15 YDR384C ATO3 hom Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
0.131 2.63E-14 YKR050W TRK2 hom Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
0.129 5.77E-14 YJR128W_d YJR128W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2
0.128 8.52E-14 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.124 4.63E-13 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
0.124 6.60E-13 YJL058C BIT61 hom Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication