YLR420W / URA4

Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR420W / URA4

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.74|P-value:1.55E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.27|P-value:1.88E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:6.68|P-value:5.38E-11 ID:SGTC_67|Compound:0335-0847|FD-Score:4.48|P-value:7.65E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:4.84|P-value:1.42E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:3.88|P-value:8.90E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:4.77|P-value:2.04E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:5.99|P-value:3.44E-9 ID:SGTC_171|Compound:k064-0041|FD-Score:4.52|P-value:6.23E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:3.36|P-value:5.97E-4 ID:SGTC_442|Compound:cape|FD-Score:-3.92|P-value:6.82E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.25|P-value:7.88E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:3.85|P-value:9.87E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:4.10|P-value:3.82E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:4.09|P-value:3.97E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:-3.30|P-value:6.70E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:-3.41|P-value:4.61E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:3.34|P-value:6.34E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.53|P-value:6.09E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:4.14|P-value:3.18E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:3.42|P-value:4.84E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.27|P-value:7.99E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:6.86|P-value:1.68E-11 ID:SGTC_1074|Compound:carmofur|FD-Score:4.79|P-value:1.84E-6 ID:SGTC_1146|Compound:3579-1023|FD-Score:4.08|P-value:4.09E-5 ID:SGTC_1160|Compound:4092-0570|FD-Score:-3.37|P-value:5.21E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.97|P-value:6.27E-5 ID:SGTC_1201|Compound:1889-3372|FD-Score:-4.19|P-value:2.34E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.42|P-value:4.33E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.43|P-value:4.58E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.93|P-value:7.35E-5 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.75|P-value:1.45E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.57|P-value:2.83E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.80|P-value:1.23E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.52|P-value:3.34E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.55|P-value:4.10E-8 ID:SGTC_1745|Compound:st081495|FD-Score:12.10|P-value:4.56E-32 ID:SGTC_1750|Compound:st081859|FD-Score:4.36|P-value:1.29E-5 ID:SGTC_1936|Compound:st074407|FD-Score:3.40|P-value:5.17E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.50|P-value:3.59E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-3.20|P-value:9.40E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-4.63|P-value:3.37E-6 ID:SGTC_2192|Compound:5915882|FD-Score:10.40|P-value:4.28E-24 ID:SGTC_2248|Compound:tofa|FD-Score:10.50|P-value:9.22E-25 ID:SGTC_2311|Compound:7694451|FD-Score:-3.28|P-value:7.07E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.68|P-value:1.93E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.22|P-value:9.43E-4 ID:SGTC_2416|Compound:tofa|FD-Score:10.20|P-value:2.10E-23 ID:SGTC_2427|Compound:5545055|FD-Score:3.56|P-value:2.89E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.29|P-value:6.88E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.45|P-value:8.76E-6 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.46|P-value:4.23E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.48|P-value:3.90E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.78|P-value:1.96E-6 ID:SGTC_2970|Compound:9090938|FD-Score:4.44|P-value:9.14E-6 ID:SGTC_3027|Compound:9089541|FD-Score:-4.25|P-value:1.83E-5 ID:SGTC_3054|Compound:9094397|FD-Score:4.64|P-value:3.61E-6 ID:SGTC_3056|Compound:9094900|FD-Score:3.55|P-value:3.10E-4 ID:SGTC_3123|Compound:9126162|FD-Score:3.78|P-value:1.31E-4 ID:SGTC_3143|Compound:9086658|FD-Score:4.07|P-value:4.27E-5 ID:SGTC_3314|Compound:9135860|FD-Score:3.49|P-value:3.73E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.74|P-value:1.55E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.27|P-value:1.88E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:6.68|P-value:5.38E-11 ID:SGTC_67|Compound:0335-0847|FD-Score:4.48|P-value:7.65E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:4.84|P-value:1.42E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:3.88|P-value:8.90E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:4.77|P-value:2.04E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:5.99|P-value:3.44E-9 ID:SGTC_171|Compound:k064-0041|FD-Score:4.52|P-value:6.23E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:3.36|P-value:5.97E-4 ID:SGTC_442|Compound:cape|FD-Score:-3.92|P-value:6.82E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.25|P-value:7.88E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:3.85|P-value:9.87E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:4.10|P-value:3.82E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:4.09|P-value:3.97E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:-3.30|P-value:6.70E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:-3.41|P-value:4.61E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:3.34|P-value:6.34E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.53|P-value:6.09E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:4.14|P-value:3.18E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:3.42|P-value:4.84E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.27|P-value:7.99E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:6.86|P-value:1.68E-11 ID:SGTC_1074|Compound:carmofur|FD-Score:4.79|P-value:1.84E-6 ID:SGTC_1146|Compound:3579-1023|FD-Score:4.08|P-value:4.09E-5 ID:SGTC_1160|Compound:4092-0570|FD-Score:-3.37|P-value:5.21E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.97|P-value:6.27E-5 ID:SGTC_1201|Compound:1889-3372|FD-Score:-4.19|P-value:2.34E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.42|P-value:4.33E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.43|P-value:4.58E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.93|P-value:7.35E-5 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.75|P-value:1.45E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.57|P-value:2.83E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.80|P-value:1.23E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.52|P-value:3.34E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.55|P-value:4.10E-8 ID:SGTC_1745|Compound:st081495|FD-Score:12.10|P-value:4.56E-32 ID:SGTC_1750|Compound:st081859|FD-Score:4.36|P-value:1.29E-5 ID:SGTC_1936|Compound:st074407|FD-Score:3.40|P-value:5.17E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.50|P-value:3.59E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-3.20|P-value:9.40E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-4.63|P-value:3.37E-6 ID:SGTC_2192|Compound:5915882|FD-Score:10.40|P-value:4.28E-24 ID:SGTC_2248|Compound:tofa|FD-Score:10.50|P-value:9.22E-25 ID:SGTC_2311|Compound:7694451|FD-Score:-3.28|P-value:7.07E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.68|P-value:1.93E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.22|P-value:9.43E-4 ID:SGTC_2416|Compound:tofa|FD-Score:10.20|P-value:2.10E-23 ID:SGTC_2427|Compound:5545055|FD-Score:3.56|P-value:2.89E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.29|P-value:6.88E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.45|P-value:8.76E-6 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.46|P-value:4.23E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.48|P-value:3.90E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.78|P-value:1.96E-6 ID:SGTC_2970|Compound:9090938|FD-Score:4.44|P-value:9.14E-6 ID:SGTC_3027|Compound:9089541|FD-Score:-4.25|P-value:1.83E-5 ID:SGTC_3054|Compound:9094397|FD-Score:4.64|P-value:3.61E-6 ID:SGTC_3056|Compound:9094900|FD-Score:3.55|P-value:3.10E-4 ID:SGTC_3123|Compound:9126162|FD-Score:3.78|P-value:1.31E-4 ID:SGTC_3143|Compound:9086658|FD-Score:4.07|P-value:4.27E-5 ID:SGTC_3314|Compound:9135860|FD-Score:3.49|P-value:3.73E-4

Top fitness defect scores for YLR420W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1745 st081495 12.10 74.0 µM 4.56E-32 RNA processing & uracil transport 3 4.26E-34
2 SGTC_2248 tofa 10.50 1.1 µM 9.22E-25 sphingolipid biosynthesis & PDR1 8 2.61E-26
3 SGTC_2192 5915882 10.40 198.5 µM 4.28E-24 RNA processing & uracil transport 1 1.34E-25
4 SGTC_2416 tofa 10.20 880.0 nM 2.10E-23 sphingolipid biosynthesis & PDR1 11 7.31E-25
5 SGTC_1072 oxiconazole 6.86 14.9 nM 1.68E-11 ergosterol depletion effects on membrane 15 3.55E-12
6 SGTC_64 000l-0018 6.68 272.1 µM 5.38E-11 ubiquinone biosynthesis & proteasome 66 1.23E-11
7 SGTC_158 k038-0087 5.99 26.4 µM 3.44E-9 ubiquinone biosynthesis & proteasome 68 1.03E-9
8 SGTC_1679 4130-1276 5.55 6.7 µM 4.10E-8 Golgi 29 1.45E-8
9 SGTC_138 0850-0259 4.84 40.9 µM 1.42E-6 cell wall 150 6.34E-7
10 SGTC_1074 carmofur 4.79 3.8 µM 1.84E-6 exosome 8 8.33E-7
11 SGTC_2947 9072639 4.78 7.7 µM 1.96E-6 tubulin folding & SWR complex 24 8.93E-7
12 SGTC_157 1348-1695 4.77 65.8 µM 2.04E-6 iron homeostasis 38 9.29E-7
13 SGTC_3054 9094397 4.64 49.5 µM 3.61E-6 fatty acid desaturase (OLE1) 14 1.71E-6
14 SGTC_951 1171-0579 4.53 82.7 µM 6.09E-6 cell wall 54 2.98E-6
15 SGTC_171 k064-0041 4.52 9.7 µM 6.23E-6 heme requiring 21 3.06E-6
16 SGTC_67 0335-0847 4.48 266.0 µM 7.65E-6 ubiquinone biosynthesis & proteasome 96 3.80E-6
17 SGTC_2629 bisabolol 4.45 100.0 µM 8.76E-6 14 4.39E-6
18 SGTC_2970 9090938 4.44 41.9 µM 9.14E-6 RNA processing & uracil transport 25 4.60E-6
19 SGTC_1750 st081859 4.36 2.4 µM 1.29E-5 endomembrane recycling 160 6.64E-6
20 SGTC_58 0957-0393 4.27 9.0 µM 1.88E-5 cell wall 347 9.94E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.291 2.57E-66 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.281 4.50E-62 YLR014C PPR1 hom Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.245 2.97E-47 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
0.234 4.37E-43 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.223 6.54E-39 YER177W BMH1 hom 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.217 3.59E-37 YKL216W URA1 hom Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
0.217 3.32E-37 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.214 3.20E-36 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.214 4.27E-36 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.206 1.99E-33 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.204 8.84E-33 YPL153C RAD53 het Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
0.200 1.73E-31 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.197 8.42E-31 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.194 6.18E-30 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.194 8.62E-30 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data