YLR421C / RPN13

Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR421C / RPN13

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.16|P-value:2.75E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:6.32|P-value:4.31E-10 ID:SGTC_605|Compound:1315-0399|FD-Score:-3.57|P-value:2.57E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.97|P-value:5.96E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:8.30|P-value:3.96E-16 ID:SGTC_1176|Compound:0848-0131|FD-Score:-3.71|P-value:1.48E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.18|P-value:9.78E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:4.04|P-value:4.52E-5 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.72|P-value:1.54E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.22|P-value:9.02E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.60|P-value:2.42E-4 ID:SGTC_1640|Compound:st009078|FD-Score:6.41|P-value:2.56E-10 ID:SGTC_1656|Compound:st012921|FD-Score:4.84|P-value:1.36E-6 ID:SGTC_1777|Compound:st045864|FD-Score:3.78|P-value:1.26E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.58|P-value:2.66E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.69|P-value:1.76E-4 ID:SGTC_1911|Compound:st060208|FD-Score:-4.16|P-value:2.59E-5 ID:SGTC_1974|Compound:st069448|FD-Score:3.27|P-value:7.60E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-3.49|P-value:3.38E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.91|P-value:7.11E-5 ID:SGTC_2376|Compound:9072922|FD-Score:-3.33|P-value:5.86E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.27|P-value:7.64E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-4.10|P-value:3.28E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:5.15|P-value:2.96E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.33|P-value:1.36E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.62|P-value:2.29E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.72|P-value:1.47E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.54|P-value:3.00E-4 ID:SGTC_2849|Compound:9019321|FD-Score:3.55|P-value:2.93E-4 ID:SGTC_2895|Compound:9054447|FD-Score:3.74|P-value:1.45E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-5.76|P-value:1.04E-8 ID:SGTC_3289|Compound:9119912|FD-Score:-3.36|P-value:5.35E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.61|P-value:2.37E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.16|P-value:2.75E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:6.32|P-value:4.31E-10 ID:SGTC_605|Compound:1315-0399|FD-Score:-3.57|P-value:2.57E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.97|P-value:5.96E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:8.30|P-value:3.96E-16 ID:SGTC_1176|Compound:0848-0131|FD-Score:-3.71|P-value:1.48E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.18|P-value:9.78E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:4.04|P-value:4.52E-5 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.72|P-value:1.54E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.22|P-value:9.02E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.60|P-value:2.42E-4 ID:SGTC_1640|Compound:st009078|FD-Score:6.41|P-value:2.56E-10 ID:SGTC_1656|Compound:st012921|FD-Score:4.84|P-value:1.36E-6 ID:SGTC_1777|Compound:st045864|FD-Score:3.78|P-value:1.26E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.58|P-value:2.66E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.69|P-value:1.76E-4 ID:SGTC_1911|Compound:st060208|FD-Score:-4.16|P-value:2.59E-5 ID:SGTC_1974|Compound:st069448|FD-Score:3.27|P-value:7.60E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-3.49|P-value:3.38E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.91|P-value:7.11E-5 ID:SGTC_2376|Compound:9072922|FD-Score:-3.33|P-value:5.86E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.27|P-value:7.64E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-4.10|P-value:3.28E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:5.15|P-value:2.96E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.33|P-value:1.36E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.62|P-value:2.29E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.72|P-value:1.47E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.54|P-value:3.00E-4 ID:SGTC_2849|Compound:9019321|FD-Score:3.55|P-value:2.93E-4 ID:SGTC_2895|Compound:9054447|FD-Score:3.74|P-value:1.45E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-5.76|P-value:1.04E-8 ID:SGTC_3289|Compound:9119912|FD-Score:-3.36|P-value:5.35E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.61|P-value:2.37E-4

Top fitness defect scores for YLR421C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1131 3454-3128 8.30 32.3 µM 3.96E-16 2 5.13E-17
2 SGTC_1640 st009078 6.41 66.4 µM 2.56E-10 1 7.40E-11
3 SGTC_103 0844-0104 6.32 51.8 µM 4.31E-10 ERAD & cell cycle 74 1.28E-10
4 SGTC_2408 sulbentine 5.15 18.4 µM 2.96E-7 30 1.30E-7
5 SGTC_1656 st012921 4.84 11.0 µM 1.36E-6 18 6.52E-7
6 SGTC_2419 paclitaxel 4.33 166.5 µM 1.36E-5 29 7.51E-6
7 SGTC_6 0844-0013 4.16 19.8 µM 2.75E-5 72 1.58E-5
8 SGTC_1439 4092-1168 4.04 46.0 µM 4.52E-5 9 2.67E-5
9 SGTC_1046 2001-0062 3.97 216.0 µM 5.96E-5 17 3.59E-5
10 SGTC_1777 st045864 3.78 34.8 µM 1.26E-4 11 7.89E-5
11 SGTC_2895 9054447 3.74 58.4 µM 1.45E-4 11 9.17E-5
12 SGTC_1477 0139-0234 3.72 16.0 µM 1.54E-4 10 9.78E-5
13 SGTC_1808 st050063 3.69 61.5 µM 1.76E-4 15 1.13E-4
14 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.62 3.7 µM 2.29E-4 112 1.49E-4
15 SGTC_3295 9122362 3.61 53.6 µM 2.37E-4 22 1.55E-4
16 SGTC_1563 conessine 3.60 56.1 µM 2.42E-4 25 1.58E-4
17 SGTC_1800 st050079 3.58 29.1 µM 2.66E-4 NEO1-PIK1 153 1.75E-4
18 SGTC_2849 9019321 3.55 13.0 µM 2.93E-4 43 1.93E-4
19 SGTC_2553 4-methylesculetin 3.54 85.1 µM 3.00E-4 35 1.98E-4
20 SGTC_1974 st069448 3.27 26.9 µM 7.60E-4 49 5.31E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 2.82E-16 YOR220W RCN2 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
0.139 7.80E-16 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.134 5.66E-15 YGR226C_d YGR226C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
0.134 6.23E-15 YGR242W_d YGR242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
0.130 4.34E-14 YLR204W QRI5 hom Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
0.130 4.38E-14 YDL037C BSC1 hom Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
0.126 2.29E-13 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.125 4.13E-13 YKL030W_d YKL030W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
0.123 9.86E-13 YDL027C_p YDL027C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
0.123 1.00E-12 YGR049W SCM4 hom Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
0.116 1.38E-11 YFL042C_p YFL042C_p hom Putative protein of unknown function; YFL042C is not an essential gene
0.113 4.37E-11 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.110 1.42E-10 YDR234W LYS4 hom Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
0.110 1.97E-10 YER038W-A_d YER038W-A_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria
0.109 2.47E-10 YOL061W PRS5 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress