YLR424W / SPP382

Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR424W / SPP382

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:9.32|P-value:6.27E-18 ID:SGTC_58|Compound:0957-0393|FD-Score:5.48|P-value:2.51E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:-3.51|P-value:6.10E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:5.34|P-value:4.97E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:6.12|P-value:1.03E-8 ID:SGTC_184|Compound:1494-0393|FD-Score:3.66|P-value:3.97E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:6.63|P-value:6.18E-10 ID:SGTC_208|Compound:k072-0098|FD-Score:3.70|P-value:3.53E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:7.45|P-value:4.12E-12 ID:SGTC_331|Compound:k048-0133|FD-Score:3.46|P-value:7.74E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.87|P-value:4.03E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:3.53|P-value:6.15E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:5.03|P-value:2.05E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:3.63|P-value:4.48E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:6.69|P-value:4.24E-10 ID:SGTC_709|Compound:3807-1509|FD-Score:4.52|P-value:1.72E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:4.19|P-value:6.16E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.49|P-value:6.92E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:4.84|P-value:4.54E-6 ID:SGTC_927|Compound:2425-1185|FD-Score:3.49|P-value:7.00E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:4.60|P-value:1.25E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.92|P-value:1.67E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.92|P-value:1.65E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.25|P-value:4.90E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:8.29|P-value:1.44E-14 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.81|P-value:5.24E-6 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.75|P-value:2.95E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.78|P-value:2.68E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.51|P-value:1.76E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.45|P-value:2.27E-5 ID:SGTC_1493|Compound:4348-0038|FD-Score:4.74|P-value:7.11E-6 ID:SGTC_1547|Compound:harmine|FD-Score:3.46|P-value:7.67E-4 ID:SGTC_1627|Compound:st003713|FD-Score:3.51|P-value:6.52E-4 ID:SGTC_1628|Compound:st004252|FD-Score:-4.89|P-value:3.47E-6 ID:SGTC_1637|Compound:st007924|FD-Score:5.48|P-value:2.49E-7 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.82|P-value:2.32E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.56|P-value:1.49E-5 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.43|P-value:8.32E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.52|P-value:6.22E-4 ID:SGTC_2049|Compound:5262831|FD-Score:4.85|P-value:4.36E-6 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.26|P-value:4.73E-5 ID:SGTC_2315|Compound:7733312|FD-Score:3.54|P-value:5.87E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.67|P-value:3.65E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.74|P-value:6.86E-6 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.45|P-value:7.91E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-5.21|P-value:8.16E-7 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.64|P-value:4.05E-4 ID:SGTC_2780|Compound:5215298|FD-Score:-3.41|P-value:8.53E-4 ID:SGTC_2790|Compound:5423970|FD-Score:5.18|P-value:1.04E-6 ID:SGTC_2864|Compound:9034755|FD-Score:5.43|P-value:3.16E-7 ID:SGTC_2912|Compound:7964298|FD-Score:4.00|P-value:1.25E-4 ID:SGTC_57|Compound:miconazole|FD-Score:9.32|P-value:6.27E-18 ID:SGTC_58|Compound:0957-0393|FD-Score:5.48|P-value:2.51E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:-3.51|P-value:6.10E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:5.34|P-value:4.97E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:6.12|P-value:1.03E-8 ID:SGTC_184|Compound:1494-0393|FD-Score:3.66|P-value:3.97E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:6.63|P-value:6.18E-10 ID:SGTC_208|Compound:k072-0098|FD-Score:3.70|P-value:3.53E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:7.45|P-value:4.12E-12 ID:SGTC_331|Compound:k048-0133|FD-Score:3.46|P-value:7.74E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.87|P-value:4.03E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:3.53|P-value:6.15E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:5.03|P-value:2.05E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:3.63|P-value:4.48E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:6.69|P-value:4.24E-10 ID:SGTC_709|Compound:3807-1509|FD-Score:4.52|P-value:1.72E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:4.19|P-value:6.16E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.49|P-value:6.92E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:4.84|P-value:4.54E-6 ID:SGTC_927|Compound:2425-1185|FD-Score:3.49|P-value:7.00E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:4.60|P-value:1.25E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.92|P-value:1.67E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.92|P-value:1.65E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.25|P-value:4.90E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:8.29|P-value:1.44E-14 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.81|P-value:5.24E-6 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.75|P-value:2.95E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.78|P-value:2.68E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.51|P-value:1.76E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.45|P-value:2.27E-5 ID:SGTC_1493|Compound:4348-0038|FD-Score:4.74|P-value:7.11E-6 ID:SGTC_1547|Compound:harmine|FD-Score:3.46|P-value:7.67E-4 ID:SGTC_1627|Compound:st003713|FD-Score:3.51|P-value:6.52E-4 ID:SGTC_1628|Compound:st004252|FD-Score:-4.89|P-value:3.47E-6 ID:SGTC_1637|Compound:st007924|FD-Score:5.48|P-value:2.49E-7 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.82|P-value:2.32E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.56|P-value:1.49E-5 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.43|P-value:8.32E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.52|P-value:6.22E-4 ID:SGTC_2049|Compound:5262831|FD-Score:4.85|P-value:4.36E-6 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.26|P-value:4.73E-5 ID:SGTC_2315|Compound:7733312|FD-Score:3.54|P-value:5.87E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.67|P-value:3.65E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.74|P-value:6.86E-6 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.45|P-value:7.91E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-5.21|P-value:8.16E-7 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.64|P-value:4.05E-4 ID:SGTC_2780|Compound:5215298|FD-Score:-3.41|P-value:8.53E-4 ID:SGTC_2790|Compound:5423970|FD-Score:5.18|P-value:1.04E-6 ID:SGTC_2864|Compound:9034755|FD-Score:5.43|P-value:3.16E-7 ID:SGTC_2912|Compound:7964298|FD-Score:4.00|P-value:1.25E-4

Top fitness defect scores for YLR424W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_57 miconazole 9.32 162.5 nM 6.27E-18 ergosterol depletion effects on membrane 8 5.94E-21 0.46
2 SGTC_1133 3473-0247 8.29 50.0 µM 1.44E-14 RSC complex & mRNA processing 12 5.71E-17 0.14
3 SGTC_268 camptothecin 7.45 39.6 µM 4.12E-12 DNA damage response 13 4.61E-14 0.09
4 SGTC_682 0kpi-0099 6.69 17.0 µM 4.24E-10 15 1.11E-11 1.00
5 SGTC_207 4185-0010 6.63 4.5 µM 6.18E-10 endomembrane recycling 6 1.73E-11 0.27
6 SGTC_161 4065-0411 6.12 162.2 µM 1.03E-8 5 4.81E-10 0.01
7 SGTC_1637 st007924 5.48 17.4 µM 2.49E-7 1 2.08E-8 0.86
8 SGTC_58 0957-0393 5.48 9.0 µM 2.51E-7 cell wall 32 2.10E-8 0.07
9 SGTC_2864 9034755 5.43 47.6 µM 3.16E-7 1 2.76E-8 0.56
10 SGTC_93 0109-0045 5.34 59.2 µM 4.97E-7 DNA damage response 6 4.72E-8 0.02
11 SGTC_2790 5423970 5.18 71.4 µM 1.04E-6 endomembrane recycling 7 1.13E-7 0.30
12 SGTC_667 1498-1044 5.03 59.0 µM 2.05E-6 RPP1 & pyrimidine depletion 4 2.51E-7 0.26
13 SGTC_580 1123-0028 4.87 41.0 µM 4.03E-6 DNA damage response 13 5.58E-7 0.20
14 SGTC_2049 5262831 4.85 67.2 µM 4.36E-6 2 6.13E-7 0.38
15 SGTC_892 0929-0076 4.84 29.0 µM 4.54E-6 TRP & mitochondrial translation 3 6.43E-7 0.49
16 SGTC_1242 0402-0103 4.81 60.1 µM 5.24E-6 3 7.62E-7 0.24
17 SGTC_2573 2',4'-dihydroxychalcone 4.74 15.0 µM 6.86E-6 8 1.05E-6 0.20
18 SGTC_1493 4348-0038 4.74 111.0 µM 7.11E-6 RSC complex & mRNA processing 27 1.09E-6 0.13
19 SGTC_1006 4099-6547 4.60 255.0 µM 1.25E-5 3 2.13E-6 0.76
20 SGTC_1745 st081495 4.56 74.0 µM 1.49E-5 RNA processing & uracil transport 22 2.60E-6 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.234 4.89E-43 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.211 6.45E-35 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.205 3.03E-33 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.205 2.97E-33 YDR172W SUP35 het Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
0.205 4.11E-33 YLR321C SFH1 het Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
0.200 1.45E-31 YMR277W FCP1 het Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
0.200 1.73E-31 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.189 3.01E-28 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.189 3.25E-28 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.185 2.56E-27 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.177 5.42E-25 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.176 1.08E-24 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.176 1.10E-24 YFL002C SPB4 het Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
0.169 5.70E-23 YBR143C SUP45 het Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
0.164 1.53E-21 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress