YLR436C / ECM30

Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR436C / ECM30

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:8.48|P-value:1.25E-15 ID:SGTC_163|Compound:1327-0036|FD-Score:4.67|P-value:6.86E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.28|P-value:3.40E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.82|P-value:3.56E-6 ID:SGTC_245|Compound:methotrexate|FD-Score:3.99|P-value:9.96E-5 ID:SGTC_397|Compound:arbidol|FD-Score:3.44|P-value:6.85E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:5.41|P-value:2.26E-7 ID:SGTC_904|Compound:1141-0088|FD-Score:3.35|P-value:9.04E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.97|P-value:1.11E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.96|P-value:1.12E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.21|P-value:4.34E-5 ID:SGTC_1162|Compound:k048-0130|FD-Score:4.16|P-value:5.23E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.59|P-value:4.17E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.98|P-value:1.06E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.34|P-value:9.28E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-3.64|P-value:3.21E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.93|P-value:1.27E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.67|P-value:3.19E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.38|P-value:8.35E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.46|P-value:6.29E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.92|P-value:1.29E-4 ID:SGTC_2244|Compound:6635614|FD-Score:5.57|P-value:1.02E-7 ID:SGTC_2246|Compound:7293507|FD-Score:-4.29|P-value:2.94E-5 ID:SGTC_2257|Compound:7960413|FD-Score:4.11|P-value:6.33E-5 ID:SGTC_2284|Compound:7945075|FD-Score:4.66|P-value:7.15E-6 ID:SGTC_2297|Compound:7971671|FD-Score:5.05|P-value:1.23E-6 ID:SGTC_2311|Compound:7694451|FD-Score:3.55|P-value:4.75E-4 ID:SGTC_2314|Compound:7727897|FD-Score:4.46|P-value:1.64E-5 ID:SGTC_2351|Compound:9026858|FD-Score:3.84|P-value:1.74E-4 ID:SGTC_2378|Compound:9074213|FD-Score:3.37|P-value:8.47E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.30|P-value:9.75E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.47|P-value:6.19E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:10.10|P-value:2.41E-21 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.46|P-value:6.32E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:5.81|P-value:3.03E-8 ID:SGTC_2730|Compound:imipramine|FD-Score:3.56|P-value:4.65E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:3.36|P-value:8.87E-4 ID:SGTC_2831|Compound:7999036|FD-Score:4.48|P-value:1.52E-5 ID:SGTC_3052|Compound:9092717|FD-Score:5.12|P-value:9.26E-7 ID:SGTC_3149|Compound:9097855|FD-Score:4.39|P-value:2.20E-5 ID:SGTC_3280|Compound:9140961|FD-Score:-3.37|P-value:7.76E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:8.48|P-value:1.25E-15 ID:SGTC_163|Compound:1327-0036|FD-Score:4.67|P-value:6.86E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.28|P-value:3.40E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.82|P-value:3.56E-6 ID:SGTC_245|Compound:methotrexate|FD-Score:3.99|P-value:9.96E-5 ID:SGTC_397|Compound:arbidol|FD-Score:3.44|P-value:6.85E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:5.41|P-value:2.26E-7 ID:SGTC_904|Compound:1141-0088|FD-Score:3.35|P-value:9.04E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.97|P-value:1.11E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.96|P-value:1.12E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.21|P-value:4.34E-5 ID:SGTC_1162|Compound:k048-0130|FD-Score:4.16|P-value:5.23E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.59|P-value:4.17E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.98|P-value:1.06E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.34|P-value:9.28E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-3.64|P-value:3.21E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.93|P-value:1.27E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.67|P-value:3.19E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.38|P-value:8.35E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.46|P-value:6.29E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.92|P-value:1.29E-4 ID:SGTC_2244|Compound:6635614|FD-Score:5.57|P-value:1.02E-7 ID:SGTC_2246|Compound:7293507|FD-Score:-4.29|P-value:2.94E-5 ID:SGTC_2257|Compound:7960413|FD-Score:4.11|P-value:6.33E-5 ID:SGTC_2284|Compound:7945075|FD-Score:4.66|P-value:7.15E-6 ID:SGTC_2297|Compound:7971671|FD-Score:5.05|P-value:1.23E-6 ID:SGTC_2311|Compound:7694451|FD-Score:3.55|P-value:4.75E-4 ID:SGTC_2314|Compound:7727897|FD-Score:4.46|P-value:1.64E-5 ID:SGTC_2351|Compound:9026858|FD-Score:3.84|P-value:1.74E-4 ID:SGTC_2378|Compound:9074213|FD-Score:3.37|P-value:8.47E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.30|P-value:9.75E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.47|P-value:6.19E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:10.10|P-value:2.41E-21 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.46|P-value:6.32E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:5.81|P-value:3.03E-8 ID:SGTC_2730|Compound:imipramine|FD-Score:3.56|P-value:4.65E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:3.36|P-value:8.87E-4 ID:SGTC_2831|Compound:7999036|FD-Score:4.48|P-value:1.52E-5 ID:SGTC_3052|Compound:9092717|FD-Score:5.12|P-value:9.26E-7 ID:SGTC_3149|Compound:9097855|FD-Score:4.39|P-value:2.20E-5 ID:SGTC_3280|Compound:9140961|FD-Score:-3.37|P-value:7.76E-4

Top fitness defect scores for YLR436C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2651 1327-0036 10.10 5.0 µM 2.41E-21 26 3.21E-24
2 SGTC_59 0330-0160 8.48 1.3 µM 1.25E-15 12 1.12E-17
3 SGTC_2727 riluzole 5.81 51.8 µM 3.03E-8 mitochondrial processes 3 3.12E-9
4 SGTC_2244 6635614 5.57 200.0 µM 1.02E-7 1 1.25E-8
5 SGTC_400 oxethazaine 5.41 37.5 µM 2.26E-7 calcium & mitochondrial duress 7 3.11E-8
6 SGTC_3052 9092717 5.12 49.5 µM 9.26E-7 8 1.56E-7
7 SGTC_2297 7971671 5.05 152.3 µM 1.23E-6 RNA processing & uracil transport 9 2.16E-7
8 SGTC_236 1509-0011 4.82 5.3 µM 3.56E-6 335 7.25E-7
9 SGTC_163 1327-0036 4.67 7.5 µM 6.86E-6 77 1.53E-6
10 SGTC_2284 7945075 4.66 39.2 µM 7.15E-6 mitochondrial processes 15 1.61E-6
11 SGTC_2831 7999036 4.48 19.5 µM 1.52E-5 30 3.81E-6
12 SGTC_2314 7727897 4.46 200.0 µM 1.64E-5 9 4.14E-6
13 SGTC_3149 9097855 4.39 49.5 µM 2.20E-5 Golgi 17 5.80E-6
14 SGTC_221 trifluoperazine 4.28 10.3 µM 3.40E-5 NEO1-PIK1 191 9.53E-6
15 SGTC_1028 k015-0029 4.21 4.8 µM 4.34E-5 17 1.26E-5
16 SGTC_1162 k048-0130 4.16 20.1 µM 5.23E-5 Golgi 17 1.56E-5
17 SGTC_2257 7960413 4.11 200.0 µM 6.33E-5 NEO1-PIK1 51 1.94E-5
18 SGTC_245 methotrexate 3.99 289.6 µM 9.96E-5 45 3.25E-5
19 SGTC_1202 1988-0068 3.98 29.6 µM 1.06E-4 RNA processing & uracil transport 25 3.49E-5
20 SGTC_907 3448-6986 3.97 75.5 µM 1.11E-4 27 3.66E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.263 3.80E-54 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.262 1.70E-53 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.205 2.48E-33 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.203 2.03E-32 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.199 2.90E-31 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.189 2.40E-28 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.167 1.97E-22 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.166 3.53E-22 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.162 3.85E-21 YIL022W TIM44 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
0.161 7.58E-21 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.156 1.15E-19 YML106W URA5 hom Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
0.154 3.25E-19 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.152 9.71E-19 YIR012W SQT1 het Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.149 3.55E-18 YNL239W LAP3 hom Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
0.148 7.25E-18 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p