YLR450W / HMG2

HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR450W / HMG2

Click on Significant Values for Screen Details ID:SGTC_45|Compound:0kpi-0036|FD-Score:-2.90|P-value:7.65E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:3.39|P-value:3.24E-5 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.17|P-value:2.58E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:2.96|P-value:2.26E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.67|P-value:2.67E-5 ID:SGTC_279|Compound:1273-0055|FD-Score:3.42|P-value:2.69E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:4.17|P-value:5.20E-7 ID:SGTC_393|Compound:acivicin|FD-Score:3.17|P-value:8.86E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:2.81|P-value:4.21E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:2.86|P-value:3.44E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:2.87|P-value:3.24E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.24|P-value:6.51E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:2.69|P-value:6.94E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:2.85|P-value:3.56E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.34|P-value:4.11E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.93|P-value:1.98E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:2.64|P-value:8.14E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:-3.05|P-value:4.26E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.89|P-value:2.48E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.40|P-value:9.43E-5 ID:SGTC_1699|Compound:st031318|FD-Score:-3.19|P-value:2.34E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.41|P-value:2.94E-5 ID:SGTC_1786|Compound:berberine|FD-Score:3.26|P-value:5.78E-5 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.23|P-value:6.61E-5 ID:SGTC_1889|Compound:st059080|FD-Score:2.82|P-value:4.08E-4 ID:SGTC_1923|Compound:st058458|FD-Score:3.46|P-value:2.30E-5 ID:SGTC_2056|Compound:5235370|FD-Score:3.05|P-value:1.53E-4 ID:SGTC_2063|Compound:5246145|FD-Score:2.74|P-value:5.60E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.08|P-value:1.31E-4 ID:SGTC_2273|Compound:7815444|FD-Score:3.41|P-value:2.86E-5 ID:SGTC_2426|Compound:5582046|FD-Score:-4.14|P-value:2.27E-6 ID:SGTC_2431|Compound:5490079|FD-Score:3.07|P-value:1.39E-4 ID:SGTC_2432|Compound:5630864|FD-Score:4.12|P-value:6.96E-7 ID:SGTC_2552|Compound:juarezic acid|FD-Score:2.68|P-value:7.09E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-2.96|P-value:6.21E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.68|P-value:7.51E-6 ID:SGTC_2761|Compound:levamisole|FD-Score:2.90|P-value:2.91E-4 ID:SGTC_2895|Compound:9054447|FD-Score:-3.42|P-value:8.49E-5 ID:SGTC_3171|Compound:9103192|FD-Score:2.62|P-value:8.96E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.02|P-value:1.18E-6 ID:SGTC_3180|Compound:9109287|FD-Score:3.58|P-value:1.27E-5 ID:SGTC_3183|Compound:9108214|FD-Score:3.51|P-value:1.76E-5 ID:SGTC_3285|Compound:9112818|FD-Score:-3.38|P-value:1.03E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-4.40|P-value:5.57E-7 ID:SGTC_3287|Compound:9117173|FD-Score:4.67|P-value:2.43E-8 ID:SGTC_3346|Compound:9158254|FD-Score:-3.26|P-value:1.78E-4 ID:SGTC_3348|Compound:9149464|FD-Score:-3.04|P-value:4.47E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:-2.90|P-value:7.65E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:3.39|P-value:3.24E-5 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.17|P-value:2.58E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:2.96|P-value:2.26E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.67|P-value:2.67E-5 ID:SGTC_279|Compound:1273-0055|FD-Score:3.42|P-value:2.69E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:4.17|P-value:5.20E-7 ID:SGTC_393|Compound:acivicin|FD-Score:3.17|P-value:8.86E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:2.81|P-value:4.21E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:2.86|P-value:3.44E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:2.87|P-value:3.24E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.24|P-value:6.51E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:2.69|P-value:6.94E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:2.85|P-value:3.56E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.34|P-value:4.11E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.93|P-value:1.98E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:2.64|P-value:8.14E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:-3.05|P-value:4.26E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.89|P-value:2.48E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.40|P-value:9.43E-5 ID:SGTC_1699|Compound:st031318|FD-Score:-3.19|P-value:2.34E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.41|P-value:2.94E-5 ID:SGTC_1786|Compound:berberine|FD-Score:3.26|P-value:5.78E-5 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.23|P-value:6.61E-5 ID:SGTC_1889|Compound:st059080|FD-Score:2.82|P-value:4.08E-4 ID:SGTC_1923|Compound:st058458|FD-Score:3.46|P-value:2.30E-5 ID:SGTC_2056|Compound:5235370|FD-Score:3.05|P-value:1.53E-4 ID:SGTC_2063|Compound:5246145|FD-Score:2.74|P-value:5.60E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.08|P-value:1.31E-4 ID:SGTC_2273|Compound:7815444|FD-Score:3.41|P-value:2.86E-5 ID:SGTC_2426|Compound:5582046|FD-Score:-4.14|P-value:2.27E-6 ID:SGTC_2431|Compound:5490079|FD-Score:3.07|P-value:1.39E-4 ID:SGTC_2432|Compound:5630864|FD-Score:4.12|P-value:6.96E-7 ID:SGTC_2552|Compound:juarezic acid|FD-Score:2.68|P-value:7.09E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-2.96|P-value:6.21E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.68|P-value:7.51E-6 ID:SGTC_2761|Compound:levamisole|FD-Score:2.90|P-value:2.91E-4 ID:SGTC_2895|Compound:9054447|FD-Score:-3.42|P-value:8.49E-5 ID:SGTC_3171|Compound:9103192|FD-Score:2.62|P-value:8.96E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.02|P-value:1.18E-6 ID:SGTC_3180|Compound:9109287|FD-Score:3.58|P-value:1.27E-5 ID:SGTC_3183|Compound:9108214|FD-Score:3.51|P-value:1.76E-5 ID:SGTC_3285|Compound:9112818|FD-Score:-3.38|P-value:1.03E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-4.40|P-value:5.57E-7 ID:SGTC_3287|Compound:9117173|FD-Score:4.67|P-value:2.43E-8 ID:SGTC_3346|Compound:9158254|FD-Score:-3.26|P-value:1.78E-4 ID:SGTC_3348|Compound:9149464|FD-Score:-3.04|P-value:4.47E-4

Top fitness defect scores for YLR450W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3287 9117173 4.67 37.1 µM 2.43E-8 60S ribosome export 2 1.52E-6
2 SGTC_327 1273-0057 4.17 4.0 µM 5.20E-7 azole & statin 28 1.55E-5
3 SGTC_2432 5630864 4.12 12.2 µM 6.96E-7 17 1.94E-5
4 SGTC_3173 9105693 4.02 49.5 µM 1.18E-6 20 2.88E-5
5 SGTC_1179 1021-1431 3.93 9.3 µM 1.98E-6 43 4.28E-5
6 SGTC_1592 4',5'-dihydroxyflavone 3.89 8.8 µM 2.48E-6 60S ribosome export 12 5.06E-5
7 SGTC_2697 mebhydrolin 3.68 72.4 µM 7.51E-6 NEO1 80 1.17E-4
8 SGTC_3180 9109287 3.58 49.5 µM 1.27E-5 19 1.74E-4
9 SGTC_3183 9108214 3.51 49.5 µM 1.76E-5 RPP1 & pyrimidine depletion 5 2.24E-4
10 SGTC_1923 st058458 3.46 19.1 µM 2.30E-5 52 2.74E-4
11 SGTC_279 1273-0055 3.42 2.9 µM 2.69E-5 azole & statin 54 3.09E-4
12 SGTC_2273 7815444 3.41 169.1 µM 2.86E-5 43 3.24E-4
13 SGTC_1752 st044819 3.41 83.0 µM 2.94E-5 38 3.31E-4
14 SGTC_86 0417-1630 3.39 471.9 µM 3.24E-5 ubiquinone biosynthesis & proteasome 186 3.56E-4
15 SGTC_1165 k072-0275 3.34 30.3 µM 4.11E-5 62 4.25E-4
16 SGTC_1786 berberine 3.26 21.6 µM 5.78E-5 60S ribosome export 67 5.51E-4
17 SGTC_877 0771-0151 3.24 33.1 µM 6.51E-5 29 6.03E-4
18 SGTC_1869 3',7'-dimethoxyflavone 3.23 40.3 µM 6.61E-5 51 6.10E-4
19 SGTC_393 acivicin 3.17 6.2 µM 8.86E-5 36 7.62E-4
20 SGTC_2108 5365811 3.08 200.0 µM 1.31E-4 55 0.00102

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 3.05E-13 YLR290C_p YLR290C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
0.118 8.02E-12 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.094 5.49E-8 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.087 3.95E-7 YLR359W ADE13 het Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
0.084 1.02E-6 YJR103W URA8 hom Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
0.082 2.15E-6 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.082 2.20E-6 YER066W_p RRT13_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.080 4.02E-6 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.079 4.52E-6 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.079 4.62E-6 YNL290W RFC3 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.075 1.27E-5 YDR181C SAS4 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.075 1.49E-5 YML076C WAR1 hom Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively
0.074 1.62E-5 YPL117C IDI1 het Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
0.073 2.47E-5 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.071 3.48E-5 YBR031W RPL4A hom Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication