YML006C / GIS4

CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YML006C / GIS4

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:7.81|P-value:3.68E-14 ID:SGTC_38|Compound:0929-0051|FD-Score:4.64|P-value:4.38E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.25|P-value:9.35E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.90|P-value:1.31E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:3.30|P-value:7.67E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:-3.97|P-value:7.37E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.83|P-value:1.26E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.89|P-value:9.43E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.85|P-value:1.13E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.03|P-value:5.52E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-6.78|P-value:4.30E-11 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-12.50|P-value:1.77E-33 ID:SGTC_501|Compound:nsc-95397|FD-Score:5.12|P-value:4.68E-7 ID:SGTC_514|Compound:4466-0038|FD-Score:6.60|P-value:1.30E-10 ID:SGTC_530|Compound:4358-1714|FD-Score:3.39|P-value:5.67E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-4.35|P-value:1.61E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:3.85|P-value:1.13E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.33|P-value:6.95E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.57|P-value:3.28E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.93|P-value:8.06E-5 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.50|P-value:3.96E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:4.30|P-value:1.91E-5 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.64|P-value:2.40E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.52|P-value:3.70E-4 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.36|P-value:6.28E-4 ID:SGTC_1602|Compound:st001878|FD-Score:4.22|P-value:2.62E-5 ID:SGTC_1616|Compound:st002734|FD-Score:3.91|P-value:8.80E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.75|P-value:1.62E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.40|P-value:5.53E-4 ID:SGTC_1886|Compound:st057356|FD-Score:6.35|P-value:5.87E-10 ID:SGTC_1892|Compound:st058411|FD-Score:6.28|P-value:8.82E-10 ID:SGTC_1896|Compound:st058441|FD-Score:3.27|P-value:8.52E-4 ID:SGTC_1899|Compound:st063254|FD-Score:3.59|P-value:2.86E-4 ID:SGTC_2204|Compound:7233102|FD-Score:3.53|P-value:3.57E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.65|P-value:2.36E-4 ID:SGTC_2376|Compound:9072922|FD-Score:3.23|P-value:9.80E-4 ID:SGTC_2483|Compound:5729050|FD-Score:4.24|P-value:2.38E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:4.41|P-value:1.16E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.79|P-value:2.23E-6 ID:SGTC_2694|Compound:st077685|FD-Score:3.35|P-value:6.55E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:4.50|P-value:8.16E-6 ID:SGTC_3328|Compound:9140624|FD-Score:3.91|P-value:8.84E-5 ID:SGTC_8|Compound:0354-0001|FD-Score:7.81|P-value:3.68E-14 ID:SGTC_38|Compound:0929-0051|FD-Score:4.64|P-value:4.38E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.25|P-value:9.35E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.90|P-value:1.31E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:3.30|P-value:7.67E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:-3.97|P-value:7.37E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.83|P-value:1.26E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.89|P-value:9.43E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.85|P-value:1.13E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.03|P-value:5.52E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-6.78|P-value:4.30E-11 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-12.50|P-value:1.77E-33 ID:SGTC_501|Compound:nsc-95397|FD-Score:5.12|P-value:4.68E-7 ID:SGTC_514|Compound:4466-0038|FD-Score:6.60|P-value:1.30E-10 ID:SGTC_530|Compound:4358-1714|FD-Score:3.39|P-value:5.67E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-4.35|P-value:1.61E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:3.85|P-value:1.13E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.33|P-value:6.95E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.57|P-value:3.28E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.93|P-value:8.06E-5 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.50|P-value:3.96E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:4.30|P-value:1.91E-5 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.64|P-value:2.40E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.52|P-value:3.70E-4 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.36|P-value:6.28E-4 ID:SGTC_1602|Compound:st001878|FD-Score:4.22|P-value:2.62E-5 ID:SGTC_1616|Compound:st002734|FD-Score:3.91|P-value:8.80E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.75|P-value:1.62E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.40|P-value:5.53E-4 ID:SGTC_1886|Compound:st057356|FD-Score:6.35|P-value:5.87E-10 ID:SGTC_1892|Compound:st058411|FD-Score:6.28|P-value:8.82E-10 ID:SGTC_1896|Compound:st058441|FD-Score:3.27|P-value:8.52E-4 ID:SGTC_1899|Compound:st063254|FD-Score:3.59|P-value:2.86E-4 ID:SGTC_2204|Compound:7233102|FD-Score:3.53|P-value:3.57E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.65|P-value:2.36E-4 ID:SGTC_2376|Compound:9072922|FD-Score:3.23|P-value:9.80E-4 ID:SGTC_2483|Compound:5729050|FD-Score:4.24|P-value:2.38E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:4.41|P-value:1.16E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.79|P-value:2.23E-6 ID:SGTC_2694|Compound:st077685|FD-Score:3.35|P-value:6.55E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:4.50|P-value:8.16E-6 ID:SGTC_3328|Compound:9140624|FD-Score:3.91|P-value:8.84E-5

Top fitness defect scores for YML006C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_8 0354-0001 7.81 70.0 µM 3.68E-14 11 2.92E-15
2 SGTC_514 4466-0038 6.60 2.6 nM 1.30E-10 cell wall 60 2.11E-11
3 SGTC_1886 st057356 6.35 49.9 µM 5.87E-10 60S ribosome export 4 1.09E-10
4 SGTC_1892 st058411 6.28 7.4 µM 8.82E-10 4 1.70E-10
5 SGTC_501 nsc-95397 5.12 161.0 µM 4.68E-7 3 1.56E-7
6 SGTC_42 0133-0086 4.90 60.6 µM 1.31E-6 8 4.78E-7
7 SGTC_2651 1327-0036 4.79 5.0 µM 2.23E-6 119 8.48E-7
8 SGTC_38 0929-0051 4.64 58.3 µM 4.38E-6 12 1.77E-6
9 SGTC_2769 toremifene 4.50 3.7 µM 8.16E-6 13 3.47E-6
10 SGTC_2574 2',4'-dihydroxychalcone 4.41 14.6 µM 1.16E-5 15 5.09E-6
11 SGTC_1198 1612-1005 4.30 86.7 µM 1.91E-5 6 8.72E-6
12 SGTC_2483 5729050 4.24 27.2 µM 2.38E-5 20 1.11E-5
13 SGTC_1602 st001878 4.22 87.6 µM 2.62E-5 16 1.23E-5
14 SGTC_453 enantio-paf c16 4.03 10.0 µM 5.52E-5 138 2.77E-5
15 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 3.93 15.5 µM 8.06E-5 27 4.17E-5
16 SGTC_1616 st002734 3.91 56.1 µM 8.80E-5 31 4.59E-5
17 SGTC_3328 9140624 3.91 29.0 µM 8.84E-5 22 4.61E-5
18 SGTC_363 0455-0021 3.89 3.1 µM 9.43E-5 azole & statin 69 4.94E-5
19 SGTC_445 e6 berbamine 3.85 66.0 µM 1.13E-4 NEO1-PIK1 114 6.01E-5
20 SGTC_683 panaxydol 3.85 45.0 µM 1.13E-4 sphingolipid biosynthesis & PDR1 44 6.02E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.182 2.81E-26 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.159 1.58E-20 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.154 3.68E-19 YLR102C APC9 hom Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.149 3.79E-18 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.145 2.96E-17 YMR298W LIP1 het Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
0.145 3.82E-17 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
0.140 4.30E-16 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.128 1.13E-13 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
0.122 1.17E-12 YJR059W PTK2 hom Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
0.122 1.18E-12 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.117 9.59E-12 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.117 1.25E-11 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.115 2.65E-11 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.113 5.38E-11 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.110 2.01E-10 YKL008C LAC1 hom Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p