YML010W / SPT5

Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML010W / SPT5

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:4.71|P-value:7.40E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.83|P-value:3.51E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.06|P-value:7.40E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.69|P-value:8.31E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.35|P-value:2.51E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.04|P-value:7.78E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.23|P-value:3.91E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:-4.05|P-value:1.35E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:-4.31|P-value:4.02E-6 ID:SGTC_211|Compound:3455-0679|FD-Score:5.55|P-value:6.79E-9 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-3.21|P-value:4.20E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-3.89|P-value:2.74E-5 ID:SGTC_349|Compound:k015-0014|FD-Score:4.09|P-value:1.47E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.45|P-value:2.22E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.30|P-value:3.91E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-3.29|P-value:3.19E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.52|P-value:1.30E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:-4.77|P-value:3.82E-7 ID:SGTC_913|Compound:3487-1245|FD-Score:3.05|P-value:9.39E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.44|P-value:1.75E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.12|P-value:7.39E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.38|P-value:2.27E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-4.53|P-value:1.34E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.39|P-value:2.76E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.81|P-value:5.03E-5 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.27|P-value:4.26E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.23|P-value:3.96E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.22|P-value:5.15E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.23|P-value:5.07E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.05|P-value:9.49E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.89|P-value:2.78E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-3.59|P-value:9.54E-5 ID:SGTC_2175|Compound:5807670|FD-Score:3.21|P-value:5.32E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.15|P-value:6.59E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.08|P-value:6.81E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.53|P-value:1.62E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.72|P-value:5.78E-5 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.88|P-value:3.79E-5 ID:SGTC_2495|Compound:cafestol|FD-Score:4.65|P-value:1.04E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.95|P-value:2.07E-7 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.51|P-value:1.35E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.28|P-value:4.50E-6 ID:SGTC_2994|Compound:9053520|FD-Score:4.20|P-value:9.13E-6 ID:SGTC_3231|Compound:9132313|FD-Score:3.11|P-value:7.77E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-3.17|P-value:4.99E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:4.71|P-value:7.40E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.83|P-value:3.51E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.06|P-value:7.40E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.69|P-value:8.31E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.35|P-value:2.51E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.04|P-value:7.78E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.23|P-value:3.91E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:-4.05|P-value:1.35E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:-4.31|P-value:4.02E-6 ID:SGTC_211|Compound:3455-0679|FD-Score:5.55|P-value:6.79E-9 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-3.21|P-value:4.20E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-3.89|P-value:2.74E-5 ID:SGTC_349|Compound:k015-0014|FD-Score:4.09|P-value:1.47E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.45|P-value:2.22E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.30|P-value:3.91E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-3.29|P-value:3.19E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.52|P-value:1.30E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:-4.77|P-value:3.82E-7 ID:SGTC_913|Compound:3487-1245|FD-Score:3.05|P-value:9.39E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.44|P-value:1.75E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.12|P-value:7.39E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.38|P-value:2.27E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-4.53|P-value:1.34E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.39|P-value:2.76E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.81|P-value:5.03E-5 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.27|P-value:4.26E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.23|P-value:3.96E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.22|P-value:5.15E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.23|P-value:5.07E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.05|P-value:9.49E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.89|P-value:2.78E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-3.59|P-value:9.54E-5 ID:SGTC_2175|Compound:5807670|FD-Score:3.21|P-value:5.32E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.15|P-value:6.59E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.08|P-value:6.81E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.53|P-value:1.62E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.72|P-value:5.78E-5 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.88|P-value:3.79E-5 ID:SGTC_2495|Compound:cafestol|FD-Score:4.65|P-value:1.04E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.95|P-value:2.07E-7 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.51|P-value:1.35E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-4.28|P-value:4.50E-6 ID:SGTC_2994|Compound:9053520|FD-Score:4.20|P-value:9.13E-6 ID:SGTC_3231|Compound:9132313|FD-Score:3.11|P-value:7.77E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-3.17|P-value:4.99E-4

Top fitness defect scores for YML010W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_211 3455-0679 5.55 143.5 µM 6.79E-9 4 1.42E-8 0.25
2 SGTC_2629 bisabolol 4.95 100.0 µM 2.07E-7 4 3.66E-7 0.34
3 SGTC_50 0929-0078 4.71 56.4 µM 7.40E-7 3 1.23E-6 0.09
4 SGTC_2495 cafestol 4.65 54.5 µM 1.04E-6 Golgi 5 1.70E-6 0.19
5 SGTC_2994 9053520 4.20 71.4 µM 9.13E-6 RPP1 & pyrimidine depletion 7 1.35E-5 0.20
6 SGTC_349 k015-0014 4.09 24.0 µM 1.47E-5 4 2.13E-5 0.07
7 SGTC_2392 N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide 3.88 200.0 µM 3.79E-5 4 5.25E-5 0.07
8 SGTC_1263 0774-1208 3.81 64.8 µM 5.03E-5 4 6.88E-5 0.07
9 SGTC_124 0136-0217 3.69 7.1 µM 8.31E-5 mitochondrial stress 29 1.11E-4 0.02
10 SGTC_161 4065-0411 3.63 162.2 µM 1.05E-4 11 1.39E-4 0.06
11 SGTC_2309 7664088 3.53 163.9 µM 1.62E-4 14 2.11E-4 0.08
12 SGTC_474 dl-dihydrosphingosine 3.45 5.0 µM 2.22E-4 12 2.84E-4 0.01
13 SGTC_1261 0712-0010 3.39 12.7 µM 2.76E-4 RNA processing & uracil transport 14 3.50E-4 0.04
14 SGTC_732 3970-1727 3.30 52.7 µM 3.91E-4 8 4.88E-4 0.02
15 SGTC_1270 0831-1170 3.27 17.3 µM 4.26E-4 3 5.31E-4 0.10
16 SGTC_1449 4112-4002 3.23 201.0 µM 5.07E-4 RPP1 & pyrimidine depletion 13 6.26E-4 0.01
17 SGTC_1381 2324-0066 3.22 62.1 µM 5.15E-4 15 6.36E-4 0.08
18 SGTC_2175 5807670 3.21 165.3 µM 5.32E-4 5 6.56E-4 0.31
19 SGTC_2177 5811945 3.15 192.0 µM 6.59E-4 8 8.06E-4 0.10
20 SGTC_1035 k081-0041 3.12 55.2 µM 7.39E-4 Golgi 14 8.98E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 5.69E-12 YOL079W_d YOL079W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.100 6.21E-9 YMR315W YMR315W hom Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress
0.090 1.52E-7 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.090 1.52E-7 YDR531W CAB1 het Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
0.086 5.34E-7 YGR230W BNS1 hom Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis
0.084 1.20E-6 YGL008C PMA1 het Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.083 1.57E-6 YJL138C TIF2 hom Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
0.082 2.05E-6 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.081 2.77E-6 YLR361C DCR2 hom Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START
0.080 3.22E-6 YOR025W HST3 hom Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.078 5.78E-6 YGR114C_d YGR114C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
0.078 7.12E-6 YCL012C YCL012C hom Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
0.077 8.23E-6 YLR043C TRX1 hom Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.076 9.90E-6 YDL172C_d YDL172C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.076 1.06E-5 YDR426C_d YDR426C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41