YML013W / UBX2

Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis

Zygosity: Homozygous strain
fixedexpanded
Profile for YML013W / UBX2

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.85|P-value:3.81E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.70|P-value:2.33E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.78|P-value:4.81E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:6.92|P-value:3.94E-10 ID:SGTC_103|Compound:0844-0104|FD-Score:3.94|P-value:2.83E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.85|P-value:3.83E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.49|P-value:5.26E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:4.41|P-value:5.37E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.85|P-value:3.76E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:4.60|P-value:2.62E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:5.87|P-value:1.00E-7 ID:SGTC_796|Compound:1234-0020|FD-Score:3.55|P-value:9.73E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:4.08|P-value:1.75E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.86|P-value:3.09E-6 ID:SGTC_902|Compound:1120-0002|FD-Score:4.85|P-value:9.70E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.74|P-value:1.52E-5 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.57|P-value:2.89E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.86|P-value:9.35E-6 ID:SGTC_1215|Compound:3852-0577|FD-Score:4.97|P-value:5.91E-6 ID:SGTC_1235|Compound:benzylparaben|FD-Score:4.42|P-value:5.04E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:5.93|P-value:7.36E-8 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.80|P-value:4.37E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.65|P-value:7.06E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:6.14|P-value:2.65E-8 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.65|P-value:3.12E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.62|P-value:2.40E-5 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.56|P-value:9.46E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.99|P-value:2.36E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:7.28|P-value:4.72E-11 ID:SGTC_1496|Compound:4469-0713|FD-Score:4.83|P-value:1.03E-5 ID:SGTC_1571|Compound:st070080|FD-Score:4.25|P-value:9.58E-5 ID:SGTC_1604|Compound:st002052|FD-Score:7.61|P-value:6.27E-12 ID:SGTC_1618|Compound:st003135|FD-Score:4.14|P-value:1.40E-4 ID:SGTC_1678|Compound:st019265|FD-Score:4.63|P-value:2.33E-5 ID:SGTC_1725|Compound:st036284|FD-Score:9.84|P-value:7.02E-19 ID:SGTC_1728|Compound:st035614|FD-Score:6.72|P-value:1.19E-9 ID:SGTC_1731|Compound:st035964|FD-Score:4.93|P-value:7.02E-6 ID:SGTC_1733|Compound:st037357|FD-Score:4.87|P-value:8.91E-6 ID:SGTC_1734|Compound:st037168|FD-Score:5.17|P-value:2.53E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.09|P-value:1.67E-4 ID:SGTC_1770|Compound:st043884|FD-Score:3.58|P-value:9.04E-4 ID:SGTC_1791|Compound:st048313|FD-Score:4.38|P-value:5.96E-5 ID:SGTC_1831|Compound:st055328|FD-Score:3.63|P-value:7.70E-4 ID:SGTC_1846|Compound:st056244|FD-Score:5.06|P-value:3.97E-6 ID:SGTC_1892|Compound:st058411|FD-Score:5.26|P-value:1.69E-6 ID:SGTC_1893|Compound:st059353|FD-Score:4.17|P-value:1.27E-4 ID:SGTC_1947|Compound:st076332|FD-Score:4.73|P-value:1.54E-5 ID:SGTC_1996|Compound:st072960|FD-Score:4.75|P-value:1.45E-5 ID:SGTC_2075|Compound:5212009|FD-Score:-4.70|P-value:6.15E-6 ID:SGTC_2079|Compound:5212404|FD-Score:-3.37|P-value:7.70E-4 ID:SGTC_2368|Compound:9071271|FD-Score:4.62|P-value:2.41E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.92|P-value:7.13E-6 ID:SGTC_2431|Compound:5490079|FD-Score:4.04|P-value:1.96E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.54|P-value:9.93E-4 ID:SGTC_2485|Compound:5633444|FD-Score:4.33|P-value:7.05E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:5.98|P-value:5.85E-8 ID:SGTC_2585|Compound:alliin|FD-Score:4.05|P-value:1.93E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.79|P-value:1.24E-5 ID:SGTC_2643|Compound:anethole|FD-Score:4.94|P-value:6.75E-6 ID:SGTC_2698|Compound:st077697|FD-Score:5.34|P-value:1.17E-6 ID:SGTC_2767|Compound:sibutramine|FD-Score:4.70|P-value:1.71E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:4.41|P-value:5.33E-5 ID:SGTC_2788|Compound:5282734|FD-Score:7.35|P-value:3.19E-11 ID:SGTC_2866|Compound:9034245|FD-Score:4.18|P-value:1.22E-4 ID:SGTC_2875|Compound:9039966|FD-Score:-3.51|P-value:4.89E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-3.38|P-value:7.51E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.76|P-value:4.98E-4 ID:SGTC_2914|Compound:7968218|FD-Score:6.87|P-value:5.25E-10 ID:SGTC_2957|Compound:9076800|FD-Score:-3.73|P-value:2.39E-4 ID:SGTC_2961|Compound:9083290|FD-Score:6.22|P-value:1.77E-8 ID:SGTC_2967|Compound:9089538|FD-Score:3.91|P-value:3.07E-4 ID:SGTC_3026|Compound:9090633|FD-Score:4.23|P-value:1.01E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.92|P-value:3.00E-4 ID:SGTC_3038|Compound:9091290|FD-Score:6.30|P-value:1.17E-8 ID:SGTC_3071|Compound:9117417|FD-Score:3.55|P-value:9.77E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.72|P-value:5.79E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.79|P-value:4.61E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.70|P-value:6.10E-4 ID:SGTC_3204|Compound:9114183|FD-Score:4.23|P-value:1.02E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.85|P-value:3.81E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.70|P-value:2.33E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.78|P-value:4.81E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:6.92|P-value:3.94E-10 ID:SGTC_103|Compound:0844-0104|FD-Score:3.94|P-value:2.83E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.85|P-value:3.83E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.49|P-value:5.26E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:4.41|P-value:5.37E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.85|P-value:3.76E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:4.60|P-value:2.62E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:5.87|P-value:1.00E-7 ID:SGTC_796|Compound:1234-0020|FD-Score:3.55|P-value:9.73E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:4.08|P-value:1.75E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.86|P-value:3.09E-6 ID:SGTC_902|Compound:1120-0002|FD-Score:4.85|P-value:9.70E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.74|P-value:1.52E-5 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.57|P-value:2.89E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.86|P-value:9.35E-6 ID:SGTC_1215|Compound:3852-0577|FD-Score:4.97|P-value:5.91E-6 ID:SGTC_1235|Compound:benzylparaben|FD-Score:4.42|P-value:5.04E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:5.93|P-value:7.36E-8 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.80|P-value:4.37E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.65|P-value:7.06E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:6.14|P-value:2.65E-8 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.65|P-value:3.12E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.62|P-value:2.40E-5 ID:SGTC_1470|Compound:k295-0292|FD-Score:3.56|P-value:9.46E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.99|P-value:2.36E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:7.28|P-value:4.72E-11 ID:SGTC_1496|Compound:4469-0713|FD-Score:4.83|P-value:1.03E-5 ID:SGTC_1571|Compound:st070080|FD-Score:4.25|P-value:9.58E-5 ID:SGTC_1604|Compound:st002052|FD-Score:7.61|P-value:6.27E-12 ID:SGTC_1618|Compound:st003135|FD-Score:4.14|P-value:1.40E-4 ID:SGTC_1678|Compound:st019265|FD-Score:4.63|P-value:2.33E-5 ID:SGTC_1725|Compound:st036284|FD-Score:9.84|P-value:7.02E-19 ID:SGTC_1728|Compound:st035614|FD-Score:6.72|P-value:1.19E-9 ID:SGTC_1731|Compound:st035964|FD-Score:4.93|P-value:7.02E-6 ID:SGTC_1733|Compound:st037357|FD-Score:4.87|P-value:8.91E-6 ID:SGTC_1734|Compound:st037168|FD-Score:5.17|P-value:2.53E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.09|P-value:1.67E-4 ID:SGTC_1770|Compound:st043884|FD-Score:3.58|P-value:9.04E-4 ID:SGTC_1791|Compound:st048313|FD-Score:4.38|P-value:5.96E-5 ID:SGTC_1831|Compound:st055328|FD-Score:3.63|P-value:7.70E-4 ID:SGTC_1846|Compound:st056244|FD-Score:5.06|P-value:3.97E-6 ID:SGTC_1892|Compound:st058411|FD-Score:5.26|P-value:1.69E-6 ID:SGTC_1893|Compound:st059353|FD-Score:4.17|P-value:1.27E-4 ID:SGTC_1947|Compound:st076332|FD-Score:4.73|P-value:1.54E-5 ID:SGTC_1996|Compound:st072960|FD-Score:4.75|P-value:1.45E-5 ID:SGTC_2075|Compound:5212009|FD-Score:-4.70|P-value:6.15E-6 ID:SGTC_2079|Compound:5212404|FD-Score:-3.37|P-value:7.70E-4 ID:SGTC_2368|Compound:9071271|FD-Score:4.62|P-value:2.41E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.92|P-value:7.13E-6 ID:SGTC_2431|Compound:5490079|FD-Score:4.04|P-value:1.96E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.54|P-value:9.93E-4 ID:SGTC_2485|Compound:5633444|FD-Score:4.33|P-value:7.05E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:5.98|P-value:5.85E-8 ID:SGTC_2585|Compound:alliin|FD-Score:4.05|P-value:1.93E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.79|P-value:1.24E-5 ID:SGTC_2643|Compound:anethole|FD-Score:4.94|P-value:6.75E-6 ID:SGTC_2698|Compound:st077697|FD-Score:5.34|P-value:1.17E-6 ID:SGTC_2767|Compound:sibutramine|FD-Score:4.70|P-value:1.71E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:4.41|P-value:5.33E-5 ID:SGTC_2788|Compound:5282734|FD-Score:7.35|P-value:3.19E-11 ID:SGTC_2866|Compound:9034245|FD-Score:4.18|P-value:1.22E-4 ID:SGTC_2875|Compound:9039966|FD-Score:-3.51|P-value:4.89E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-3.38|P-value:7.51E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.76|P-value:4.98E-4 ID:SGTC_2914|Compound:7968218|FD-Score:6.87|P-value:5.25E-10 ID:SGTC_2957|Compound:9076800|FD-Score:-3.73|P-value:2.39E-4 ID:SGTC_2961|Compound:9083290|FD-Score:6.22|P-value:1.77E-8 ID:SGTC_2967|Compound:9089538|FD-Score:3.91|P-value:3.07E-4 ID:SGTC_3026|Compound:9090633|FD-Score:4.23|P-value:1.01E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.92|P-value:3.00E-4 ID:SGTC_3038|Compound:9091290|FD-Score:6.30|P-value:1.17E-8 ID:SGTC_3071|Compound:9117417|FD-Score:3.55|P-value:9.77E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.72|P-value:5.79E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.79|P-value:4.61E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.70|P-value:6.10E-4 ID:SGTC_3204|Compound:9114183|FD-Score:4.23|P-value:1.02E-4

Top fitness defect scores for YML013W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1725 st036284 9.84 20.6 µM 7.02E-19 1 3.64E-23
2 SGTC_1604 st002052 7.61 72.4 µM 6.27E-12 TSC3-RPN4 3 1.37E-14
3 SGTC_2788 5282734 7.35 71.4 µM 3.19E-11 5 1.03E-13
4 SGTC_1491 4138-0063 7.28 26.5 µM 4.72E-11 1 1.67E-13
5 SGTC_86 0417-1630 6.92 471.9 µM 3.94E-10 ubiquinone biosynthesis & proteasome 72 2.29E-12
6 SGTC_2914 7968218 6.87 45.3 µM 5.25E-10 1 3.27E-12
7 SGTC_1728 st035614 6.72 33.8 µM 1.19E-9 6 8.99E-12
8 SGTC_3038 9091290 6.30 49.5 µM 1.17E-8 3 1.52E-10
9 SGTC_2961 9083290 6.22 32.5 µM 1.77E-8 1 2.53E-10
10 SGTC_1346 1488-2224 6.14 62.4 µM 2.65E-8 6 4.18E-10
11 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 5.98 63.6 µM 5.85E-8 13 1.11E-9
12 SGTC_1238 0333-0287 5.93 48.8 µM 7.36E-8 Golgi 3 1.48E-9
13 SGTC_733 4069-0007 5.87 56.1 µM 1.00E-7 ERAD & cell cycle 42 2.17E-9
14 SGTC_64 000l-0018 5.70 272.1 µM 2.33E-7 ubiquinone biosynthesis & proteasome 86 6.16E-9
15 SGTC_2698 st077697 5.34 67.5 µM 1.17E-6 6 4.56E-8
16 SGTC_1892 st058411 5.26 7.4 µM 1.69E-6 8 7.21E-8
17 SGTC_1734 st037168 5.17 69.4 µM 2.53E-6 7 1.19E-7
18 SGTC_1846 st056244 5.06 14.8 µM 3.97E-6 8 2.07E-7
19 SGTC_1215 3852-0577 4.97 11.2 µM 5.91E-6 8 3.40E-7
20 SGTC_2643 anethole 4.94 100.0 µM 6.75E-6 6 4.00E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 5.74E-33 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.179 1.33E-25 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.169 5.71E-23 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.144 7.13E-17 YDL020C RPN4 hom Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
0.133 8.93E-15 YMR264W CUE1 hom Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
0.133 1.15E-14 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.128 8.76E-14 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.128 1.11E-13 YDR200C VPS64 hom Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
0.121 2.05E-12 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.120 2.47E-12 YML012C-A_d YML012C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
0.119 5.49E-12 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.117 9.37E-12 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.116 1.49E-11 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.114 3.52E-11 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.114 4.28E-11 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover