YML018C

Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YML018C / YML018C

Click on Significant Values for Screen Details

Top fitness defect scores for YML018C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2620 irigenin 8.78 100.0 µM 3.38E-18 6 8.03E-19
2 SGTC_1576 erythromycin ethylsuccinate 5.44 23.2 µM 4.49E-8 6 2.64E-8
3 SGTC_145 3737-0113 5.25 38.4 µM 1.27E-7 DNA intercalators 36 7.80E-8
4 SGTC_1926 methyl fluorone black 5.02 53.6 µM 4.11E-7 DNA intercalators 35 2.63E-7
5 SGTC_1748 st037455 4.99 53.6 µM 4.71E-7 9 3.03E-7
6 SGTC_3126 9127922 4.94 49.5 µM 5.88E-7 fatty acid desaturase (OLE1) 24 3.81E-7
7 SGTC_184 1494-0393 4.69 24.7 µM 2.05E-6 42 1.39E-6
8 SGTC_94 0334-0113 4.55 514.8 µM 3.79E-6 11 2.63E-6
9 SGTC_2923 7992654 4.36 58.1 µM 9.05E-6 Golgi 17 6.49E-6
10 SGTC_2996 9066192 4.29 71.4 µM 1.23E-5 25 8.91E-6
11 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 4.28 15.5 µM 1.30E-5 22 9.43E-6
12 SGTC_2241 6772625 4.27 37.8 µM 1.35E-5 18 9.84E-6
13 SGTC_100 0086-0128 4.24 49.6 µM 1.50E-5 copper-dependent oxidative stress 39 1.09E-5
14 SGTC_1302 1159-0335 4.21 255.0 µM 1.71E-5 10 1.25E-5
15 SGTC_1909 st060837 4.17 51.5 µM 2.06E-5 44 1.52E-5
16 SGTC_2857 9024691 4.05 58.4 µM 3.34E-5 23 2.51E-5
17 SGTC_42 0133-0086 4.00 60.6 µM 4.20E-5 31 3.19E-5
18 SGTC_1611 st001856 3.93 99.4 µM 5.57E-5 18 4.26E-5
19 SGTC_1867 st057372 3.92 64.0 µM 5.83E-5 8 4.47E-5
20 SGTC_1098 clofazimine 3.88 15.1 µM 6.90E-5 heme biosynthesis & mitochondrial translocase 23 5.33E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 6.36E-21 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.152 1.13E-18 YPL035C_d YPL035C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
0.142 1.28E-16 YBR182C-A_p YBR182C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.128 1.03E-13 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.128 1.16E-13 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.127 1.65E-13 YMR044W IOC4 hom Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
0.127 2.07E-13 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.126 2.95E-13 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.117 9.45E-12 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.116 2.06E-11 YDR158W HOM2 hom Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.115 2.33E-11 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.114 4.00E-11 YCR057C PWP2 het Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
0.108 3.20E-10 YMR030W RSF1 hom Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.106 8.76E-10 YPL197C_d YPL197C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
0.103 2.55E-9 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W