YML025C / YML6

Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML025C / YML6

Click on Significant Values for Screen Details ID:SGTC_54|Compound:0159-0055|FD-Score:3.32|P-value:3.95E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.05|P-value:7.66E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-3.15|P-value:5.51E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.25|P-value:4.95E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.23|P-value:5.32E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:5.23|P-value:5.36E-8 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.38|P-value:3.13E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-15.80|P-value:2.00E-59 ID:SGTC_893|Compound:0958-0369|FD-Score:-10.60|P-value:3.70E-28 ID:SGTC_1089|Compound:terbinafine|FD-Score:-3.35|P-value:2.64E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.78|P-value:4.04E-7 ID:SGTC_1276|Compound:0871-0068|FD-Score:-5.84|P-value:8.82E-10 ID:SGTC_1365|Compound:1672-3266|FD-Score:-4.19|P-value:7.39E-6 ID:SGTC_1367|Compound:1812-0510|FD-Score:-10.70|P-value:1.91E-28 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.60|P-value:9.82E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.21|P-value:4.40E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.88|P-value:4.22E-5 ID:SGTC_1819|Compound:st053448|FD-Score:3.18|P-value:6.39E-4 ID:SGTC_1978|Compound:st071761|FD-Score:3.55|P-value:1.62E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.63|P-value:1.29E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.79|P-value:4.34E-5 ID:SGTC_2415|Compound:st077333|FD-Score:-3.67|P-value:7.34E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:4.79|P-value:5.65E-7 ID:SGTC_2835|Compound:9006588|FD-Score:5.00|P-value:1.88E-7 ID:SGTC_2962|Compound:9082311|FD-Score:4.12|P-value:1.43E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_3108|Compound:9121785|FD-Score:4.76|P-value:6.70E-7 ID:SGTC_3111|Compound:9122088|FD-Score:-3.40|P-value:2.11E-4 ID:SGTC_3121|Compound:9125696|FD-Score:3.48|P-value:2.15E-4 ID:SGTC_3127|Compound:9126946|FD-Score:4.11|P-value:1.49E-5 ID:SGTC_3230|Compound:9131665|FD-Score:-3.90|P-value:2.76E-5 ID:SGTC_3245|Compound:9134821|FD-Score:3.77|P-value:6.48E-5 ID:SGTC_3288|Compound:9119690|FD-Score:3.50|P-value:1.99E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-3.04|P-value:8.07E-4 ID:SGTC_3354|Compound:9151612|FD-Score:-3.18|P-value:4.96E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:3.32|P-value:3.95E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.05|P-value:7.66E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-3.15|P-value:5.51E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.25|P-value:4.95E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.23|P-value:5.32E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:5.23|P-value:5.36E-8 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.38|P-value:3.13E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-15.80|P-value:2.00E-59 ID:SGTC_893|Compound:0958-0369|FD-Score:-10.60|P-value:3.70E-28 ID:SGTC_1089|Compound:terbinafine|FD-Score:-3.35|P-value:2.64E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.78|P-value:4.04E-7 ID:SGTC_1276|Compound:0871-0068|FD-Score:-5.84|P-value:8.82E-10 ID:SGTC_1365|Compound:1672-3266|FD-Score:-4.19|P-value:7.39E-6 ID:SGTC_1367|Compound:1812-0510|FD-Score:-10.70|P-value:1.91E-28 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.60|P-value:9.82E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.21|P-value:4.40E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.88|P-value:4.22E-5 ID:SGTC_1819|Compound:st053448|FD-Score:3.18|P-value:6.39E-4 ID:SGTC_1978|Compound:st071761|FD-Score:3.55|P-value:1.62E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.63|P-value:1.29E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.79|P-value:4.34E-5 ID:SGTC_2415|Compound:st077333|FD-Score:-3.67|P-value:7.34E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:4.79|P-value:5.65E-7 ID:SGTC_2835|Compound:9006588|FD-Score:5.00|P-value:1.88E-7 ID:SGTC_2962|Compound:9082311|FD-Score:4.12|P-value:1.43E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_3108|Compound:9121785|FD-Score:4.76|P-value:6.70E-7 ID:SGTC_3111|Compound:9122088|FD-Score:-3.40|P-value:2.11E-4 ID:SGTC_3121|Compound:9125696|FD-Score:3.48|P-value:2.15E-4 ID:SGTC_3127|Compound:9126946|FD-Score:4.11|P-value:1.49E-5 ID:SGTC_3230|Compound:9131665|FD-Score:-3.90|P-value:2.76E-5 ID:SGTC_3245|Compound:9134821|FD-Score:3.77|P-value:6.48E-5 ID:SGTC_3288|Compound:9119690|FD-Score:3.50|P-value:1.99E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-3.04|P-value:8.07E-4 ID:SGTC_3354|Compound:9151612|FD-Score:-3.18|P-value:4.96E-4

Top fitness defect scores for YML025C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_501 nsc-95397 5.23 161.0 µM 5.36E-8 2 8.63E-8 0.35
2 SGTC_2835 9006588 5.00 71.4 µM 1.88E-7 3 2.88E-7 0.29
3 SGTC_2422 chlorphenesin carbamate 4.79 384.2 µM 5.65E-7 2 8.31E-7 0.37
4 SGTC_3108 9121785 4.76 49.5 µM 6.70E-7 4 9.80E-7 0.13
5 SGTC_2160 5660466 4.63 132.9 µM 1.29E-6 60S ribosome export 8 1.85E-6 0.14
6 SGTC_2962 9082311 4.12 13.2 µM 1.43E-5 9 1.86E-5 0.05
7 SGTC_3127 9126946 4.11 49.5 µM 1.49E-5 ERG2 10 1.94E-5 0.22
8 SGTC_1747 st037496 3.88 66.6 µM 4.22E-5 7 5.29E-5 0.06
9 SGTC_3245 9134821 3.77 49.5 µM 6.48E-5 4 8.00E-5 0.10
10 SGTC_3052 9092717 3.63 49.5 µM 1.16E-4 4 1.40E-4 0.05
11 SGTC_1978 st071761 3.55 42.0 µM 1.62E-4 TSC3-RPN4 9 1.93E-4 0.01
12 SGTC_3288 9119690 3.50 36.2 µM 1.99E-4 22 2.36E-4 0.09
13 SGTC_3121 9125696 3.48 49.5 µM 2.15E-4 3 2.55E-4 0.42
14 SGTC_511 blebbistatin 3.38 171.0 µM 3.13E-4 DNA intercalators 10 3.65E-4 0.14
15 SGTC_54 0159-0055 3.32 9.0 µM 3.95E-4 ERG2 11 4.58E-4 0.14
16 SGTC_391 gliotoxin 3.25 8.3 µM 4.95E-4 9 5.69E-4 0.02
17 SGTC_473 2-arachidonoylglycerol 3.23 10.0 µM 5.32E-4 11 6.10E-4 0.02
18 SGTC_1819 st053448 3.18 58.6 µM 6.39E-4 amide catabolism 5 7.28E-4 0.01
19 SGTC_2163 5671233 3.05 125.0 µM 0.00102 28 0.00115 0.01
20 SGTC_44 0929-0055 3.02 61.2 µM 0.00111 20 0.00125 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.315 2.61E-78 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.264 1.77E-54 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.262 1.35E-53 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.256 1.94E-51 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.239 1.26E-44 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.231 1.09E-41 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.230 1.18E-41 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.225 1.09E-39 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.222 9.82E-39 YDR242W AMD2 hom Putative amidase
0.215 2.96E-36 YGR251W NOP19 het Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
0.201 4.78E-32 YPR088C SRP54 het Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
0.199 2.97E-31 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.194 8.39E-30 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.193 1.45E-29 YIR035C_p YIR035C_p hom Putative cytoplasmic short-chain dehydrogenase/reductase
0.188 4.01E-28 YNR070W_p PDR18_p hom Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies