YML026C / RPS18B

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YML026C / RPS18B

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-4.95|P-value:1.34E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:-4.20|P-value:1.93E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:5.36|P-value:1.28E-6 ID:SGTC_316|Compound:k060-0003|FD-Score:3.82|P-value:3.78E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:5.53|P-value:6.26E-7 ID:SGTC_335|Compound:4077-0256|FD-Score:5.13|P-value:3.36E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:4.10|P-value:1.50E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:-4.61|P-value:4.76E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.39|P-value:1.00E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.64|P-value:6.49E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.51|P-value:3.61E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:-4.28|P-value:1.49E-4 ID:SGTC_846|Compound:0335-0850|FD-Score:-4.44|P-value:8.51E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.66|P-value:6.06E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.58|P-value:7.75E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:6.13|P-value:3.98E-8 ID:SGTC_1203|Compound:3992-3522|FD-Score:6.94|P-value:6.69E-10 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.06|P-value:3.01E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:5.47|P-value:8.02E-7 ID:SGTC_1451|Compound:4152-0210|FD-Score:-5.63|P-value:8.64E-7 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.87|P-value:9.37E-6 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.27|P-value:8.40E-5 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.42|P-value:4.99E-5 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.70|P-value:5.37E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.62|P-value:6.91E-4 ID:SGTC_1834|Compound:st055981|FD-Score:-4.50|P-value:6.98E-5 ID:SGTC_1839|Compound:st053978|FD-Score:3.59|P-value:7.42E-4 ID:SGTC_1864|Compound:st057645|FD-Score:5.17|P-value:2.79E-6 ID:SGTC_1870|Compound:st057514|FD-Score:3.70|P-value:5.37E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.87|P-value:3.13E-4 ID:SGTC_2057|Compound:5236571|FD-Score:4.33|P-value:6.99E-5 ID:SGTC_2058|Compound:5236698|FD-Score:4.40|P-value:5.37E-5 ID:SGTC_2165|Compound:5727197|FD-Score:16.80|P-value:2.37E-48 ID:SGTC_2275|Compound:7947463|FD-Score:-6.36|P-value:3.00E-8 ID:SGTC_2381|Compound:9035592|FD-Score:3.62|P-value:6.93E-4 ID:SGTC_2475|Compound:5812728|FD-Score:4.17|P-value:1.18E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:10.10|P-value:8.09E-19 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:9.21|P-value:5.15E-16 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.86|P-value:3.29E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-3.73|P-value:8.26E-4 ID:SGTC_2657|Compound:nystatin|FD-Score:3.88|P-value:3.13E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.64|P-value:6.50E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.65|P-value:2.98E-9 ID:SGTC_2761|Compound:levamisole|FD-Score:4.78|P-value:1.35E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.83|P-value:6.21E-4 ID:SGTC_2855|Compound:9016525|FD-Score:4.64|P-value:2.25E-5 ID:SGTC_2992|Compound:9074591|FD-Score:4.27|P-value:8.54E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.71|P-value:8.68E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.95|P-value:1.34E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:-4.20|P-value:1.93E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:5.36|P-value:1.28E-6 ID:SGTC_316|Compound:k060-0003|FD-Score:3.82|P-value:3.78E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:5.53|P-value:6.26E-7 ID:SGTC_335|Compound:4077-0256|FD-Score:5.13|P-value:3.36E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:4.10|P-value:1.50E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:-4.61|P-value:4.76E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.39|P-value:1.00E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.64|P-value:6.49E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.51|P-value:3.61E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:-4.28|P-value:1.49E-4 ID:SGTC_846|Compound:0335-0850|FD-Score:-4.44|P-value:8.51E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.66|P-value:6.06E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.58|P-value:7.75E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:6.13|P-value:3.98E-8 ID:SGTC_1203|Compound:3992-3522|FD-Score:6.94|P-value:6.69E-10 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.06|P-value:3.01E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:5.47|P-value:8.02E-7 ID:SGTC_1451|Compound:4152-0210|FD-Score:-5.63|P-value:8.64E-7 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.87|P-value:9.37E-6 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.27|P-value:8.40E-5 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.42|P-value:4.99E-5 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.70|P-value:5.37E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.62|P-value:6.91E-4 ID:SGTC_1834|Compound:st055981|FD-Score:-4.50|P-value:6.98E-5 ID:SGTC_1839|Compound:st053978|FD-Score:3.59|P-value:7.42E-4 ID:SGTC_1864|Compound:st057645|FD-Score:5.17|P-value:2.79E-6 ID:SGTC_1870|Compound:st057514|FD-Score:3.70|P-value:5.37E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.87|P-value:3.13E-4 ID:SGTC_2057|Compound:5236571|FD-Score:4.33|P-value:6.99E-5 ID:SGTC_2058|Compound:5236698|FD-Score:4.40|P-value:5.37E-5 ID:SGTC_2165|Compound:5727197|FD-Score:16.80|P-value:2.37E-48 ID:SGTC_2275|Compound:7947463|FD-Score:-6.36|P-value:3.00E-8 ID:SGTC_2381|Compound:9035592|FD-Score:3.62|P-value:6.93E-4 ID:SGTC_2475|Compound:5812728|FD-Score:4.17|P-value:1.18E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:10.10|P-value:8.09E-19 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:9.21|P-value:5.15E-16 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.86|P-value:3.29E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-3.73|P-value:8.26E-4 ID:SGTC_2657|Compound:nystatin|FD-Score:3.88|P-value:3.13E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.64|P-value:6.50E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.65|P-value:2.98E-9 ID:SGTC_2761|Compound:levamisole|FD-Score:4.78|P-value:1.35E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.83|P-value:6.21E-4 ID:SGTC_2855|Compound:9016525|FD-Score:4.64|P-value:2.25E-5 ID:SGTC_2992|Compound:9074591|FD-Score:4.27|P-value:8.54E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.71|P-value:8.68E-4

Top fitness defect scores for YML026C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2165 5727197 16.80 32.1 µM 2.37E-48 1 1.60E-63
2 SGTC_2598 perillyl alcohol 10.10 100.0 µM 8.09E-19 2 3.22E-24
3 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 9.21 100.0 µM 5.15E-16 1 1.67E-20
4 SGTC_1203 3992-3522 6.94 103.0 µM 6.69E-10 5 2.01E-12
5 SGTC_2688 kanamycin b 6.65 96.7 µM 2.98E-9 calcium & mitochondrial duress 120 1.44E-11
6 SGTC_1182 1222-0449 6.13 220.0 µM 3.98E-8 3 4.35E-10
7 SGTC_318 1488-0629 5.53 67.2 µM 6.26E-7 copper-dependent oxidative stress 12 1.62E-8
8 SGTC_1446 4239-0190 5.47 62.0 µM 8.02E-7 10 2.24E-8
9 SGTC_118 0083-0097 5.36 135.3 µM 1.28E-6 translation 43 4.12E-8
10 SGTC_1864 st057645 5.17 30.4 µM 2.79E-6 amide catabolism 2 1.14E-7
11 SGTC_335 4077-0256 5.13 27.6 µM 3.36E-6 7 1.46E-7
12 SGTC_1457 k015-0032 4.87 57.3 µM 9.37E-6 16 5.57E-7
13 SGTC_2761 levamisole 4.78 97.9 µM 1.35E-5 6 8.95E-7
14 SGTC_2855 9016525 4.64 58.4 µM 2.25E-5 11 1.74E-6
15 SGTC_800 3448-7362 4.51 155.0 µM 3.61E-5 translation 117 3.23E-6
16 SGTC_1511 0906-3109 4.42 28.5 µM 4.99E-5 excess fatty acid 29 4.91E-6
17 SGTC_2058 5236698 4.40 133.0 µM 5.37E-5 18 5.41E-6
18 SGTC_2057 5236571 4.33 38.3 µM 6.99E-5 RPP1 & pyrimidine depletion 21 7.62E-6
19 SGTC_1510 1123-0200 4.27 71.5 µM 8.40E-5 34 9.69E-6
20 SGTC_2992 9074591 4.27 71.4 µM 8.54E-5 13 9.89E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 9.16E-15 YPL213W LEA1 hom Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
0.128 1.07E-13 YLR264W RPS28B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
0.123 8.23E-13 YLR393W ATP10 hom Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6
0.123 1.04E-12 YAL007C ERP2 hom Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles
0.113 5.48E-11 YOR233W KIN4 hom Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.113 5.60E-11 YPR171W BSP1 hom Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton
0.106 7.73E-10 YNR066C_p YNR066C_p hom Putative membrane-localized protein of unknown function
0.105 9.29E-10 YLR062C_d BUD28_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.104 1.44E-9 YGL127C SOH1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
0.101 4.97E-9 YKL043W PHD1 hom Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
0.101 5.50E-9 YDL061C RPS29B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
0.098 1.24E-8 YDR450W RPS18A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress
0.098 1.44E-8 YPL141C FRK1 hom Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
0.098 1.59E-8 YGL126W SCS3 hom Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
0.096 2.22E-8 YHR162W MPC2 hom Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium