YML028W / TSA1

Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YML028W / TSA1

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:4.73|P-value:3.37E-7 ID:SGTC_68|Compound:0986-0284|FD-Score:6.17|P-value:4.57E-11 ID:SGTC_103|Compound:0844-0104|FD-Score:3.13|P-value:5.21E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.45|P-value:1.36E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.90|P-value:2.12E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:2.99|P-value:8.64E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.73|P-value:4.58E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:3.61|P-value:7.47E-5 ID:SGTC_394|Compound:menadione|FD-Score:3.50|P-value:1.18E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.00|P-value:7.75E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.15|P-value:4.73E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.93|P-value:1.69E-5 ID:SGTC_444|Compound:curcumin|FD-Score:3.94|P-value:1.78E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:8.10|P-value:8.67E-18 ID:SGTC_470|Compound:aa-861|FD-Score:3.08|P-value:6.23E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.29|P-value:2.77E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:3.75|P-value:4.09E-5 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:2.97|P-value:9.13E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.34|P-value:2.29E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.57|P-value:9.15E-5 ID:SGTC_659|Compound:k018-0002|FD-Score:4.96|P-value:9.78E-8 ID:SGTC_694|Compound:1315-0438|FD-Score:4.71|P-value:3.82E-7 ID:SGTC_699|Compound:0375-0592|FD-Score:3.07|P-value:6.32E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.96|P-value:1.61E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.24|P-value:4.04E-6 ID:SGTC_763|Compound:3453-1109|FD-Score:3.27|P-value:3.07E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.59|P-value:8.29E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.45|P-value:5.33E-9 ID:SGTC_1078|Compound:idebenone|FD-Score:3.97|P-value:1.58E-5 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.60|P-value:2.01E-9 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.70|P-value:5.12E-5 ID:SGTC_1170|Compound:0310-0127|FD-Score:3.01|P-value:7.91E-4 ID:SGTC_1422|Compound:4031-0024|FD-Score:4.15|P-value:6.60E-6 ID:SGTC_1530|Compound:rhein|FD-Score:3.26|P-value:3.11E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.59|P-value:8.43E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.15|P-value:4.54E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.07|P-value:6.47E-4 ID:SGTC_1731|Compound:st035964|FD-Score:6.69|P-value:1.09E-12 ID:SGTC_1740|Compound:st038286|FD-Score:-3.34|P-value:2.12E-4 ID:SGTC_1881|Compound:5652484|FD-Score:3.29|P-value:2.74E-4 ID:SGTC_1960|Compound:st011366|FD-Score:-3.02|P-value:7.36E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.34|P-value:2.28E-4 ID:SGTC_2050|Compound:5263056|FD-Score:2.96|P-value:9.65E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.11|P-value:5.59E-4 ID:SGTC_2198|Compound:6589944|FD-Score:4.98|P-value:8.75E-8 ID:SGTC_2256|Compound:7954252|FD-Score:3.98|P-value:1.45E-5 ID:SGTC_2284|Compound:7945075|FD-Score:3.53|P-value:1.06E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.54|P-value:1.03E-4 ID:SGTC_2318|Compound:6029776|FD-Score:-3.05|P-value:6.51E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.14|P-value:4.59E-4 ID:SGTC_2328|Compound:6201408|FD-Score:5.50|P-value:3.96E-9 ID:SGTC_2368|Compound:9071271|FD-Score:4.58|P-value:7.47E-7 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.20|P-value:3.96E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.49|P-value:1.24E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:8.17|P-value:4.61E-18 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.04|P-value:6.75E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.75|P-value:6.93E-13 ID:SGTC_2586|Compound:curcumin|FD-Score:3.20|P-value:3.92E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.31|P-value:2.62E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.22|P-value:3.64E-4 ID:SGTC_2681|Compound:menadione|FD-Score:5.40|P-value:7.04E-9 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.13|P-value:5.10E-4 ID:SGTC_2781|Compound:5567276|FD-Score:4.13|P-value:7.23E-6 ID:SGTC_2802|Compound:7925815|FD-Score:-3.94|P-value:1.61E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.21|P-value:3.84E-4 ID:SGTC_2968|Compound:9090663|FD-Score:4.58|P-value:7.53E-7 ID:SGTC_3308|Compound:9128332|FD-Score:3.38|P-value:1.99E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.13|P-value:7.47E-6 ID:SGTC_29|Compound:0299-0004|FD-Score:4.73|P-value:3.37E-7 ID:SGTC_68|Compound:0986-0284|FD-Score:6.17|P-value:4.57E-11 ID:SGTC_103|Compound:0844-0104|FD-Score:3.13|P-value:5.21E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.45|P-value:1.36E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.90|P-value:2.12E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:2.99|P-value:8.64E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.73|P-value:4.58E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:3.61|P-value:7.47E-5 ID:SGTC_394|Compound:menadione|FD-Score:3.50|P-value:1.18E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.00|P-value:7.75E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.15|P-value:4.73E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.93|P-value:1.69E-5 ID:SGTC_444|Compound:curcumin|FD-Score:3.94|P-value:1.78E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:8.10|P-value:8.67E-18 ID:SGTC_470|Compound:aa-861|FD-Score:3.08|P-value:6.23E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.29|P-value:2.77E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:3.75|P-value:4.09E-5 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:2.97|P-value:9.13E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.34|P-value:2.29E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.57|P-value:9.15E-5 ID:SGTC_659|Compound:k018-0002|FD-Score:4.96|P-value:9.78E-8 ID:SGTC_694|Compound:1315-0438|FD-Score:4.71|P-value:3.82E-7 ID:SGTC_699|Compound:0375-0592|FD-Score:3.07|P-value:6.32E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.96|P-value:1.61E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.24|P-value:4.04E-6 ID:SGTC_763|Compound:3453-1109|FD-Score:3.27|P-value:3.07E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.59|P-value:8.29E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.45|P-value:5.33E-9 ID:SGTC_1078|Compound:idebenone|FD-Score:3.97|P-value:1.58E-5 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.60|P-value:2.01E-9 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.70|P-value:5.12E-5 ID:SGTC_1170|Compound:0310-0127|FD-Score:3.01|P-value:7.91E-4 ID:SGTC_1422|Compound:4031-0024|FD-Score:4.15|P-value:6.60E-6 ID:SGTC_1530|Compound:rhein|FD-Score:3.26|P-value:3.11E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.59|P-value:8.43E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.15|P-value:4.54E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.07|P-value:6.47E-4 ID:SGTC_1731|Compound:st035964|FD-Score:6.69|P-value:1.09E-12 ID:SGTC_1740|Compound:st038286|FD-Score:-3.34|P-value:2.12E-4 ID:SGTC_1881|Compound:5652484|FD-Score:3.29|P-value:2.74E-4 ID:SGTC_1960|Compound:st011366|FD-Score:-3.02|P-value:7.36E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.34|P-value:2.28E-4 ID:SGTC_2050|Compound:5263056|FD-Score:2.96|P-value:9.65E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.11|P-value:5.59E-4 ID:SGTC_2198|Compound:6589944|FD-Score:4.98|P-value:8.75E-8 ID:SGTC_2256|Compound:7954252|FD-Score:3.98|P-value:1.45E-5 ID:SGTC_2284|Compound:7945075|FD-Score:3.53|P-value:1.06E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.54|P-value:1.03E-4 ID:SGTC_2318|Compound:6029776|FD-Score:-3.05|P-value:6.51E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.14|P-value:4.59E-4 ID:SGTC_2328|Compound:6201408|FD-Score:5.50|P-value:3.96E-9 ID:SGTC_2368|Compound:9071271|FD-Score:4.58|P-value:7.47E-7 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.20|P-value:3.96E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.49|P-value:1.24E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:8.17|P-value:4.61E-18 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.04|P-value:6.75E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.75|P-value:6.93E-13 ID:SGTC_2586|Compound:curcumin|FD-Score:3.20|P-value:3.92E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.31|P-value:2.62E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.22|P-value:3.64E-4 ID:SGTC_2681|Compound:menadione|FD-Score:5.40|P-value:7.04E-9 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.13|P-value:5.10E-4 ID:SGTC_2781|Compound:5567276|FD-Score:4.13|P-value:7.23E-6 ID:SGTC_2802|Compound:7925815|FD-Score:-3.94|P-value:1.61E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.21|P-value:3.84E-4 ID:SGTC_2968|Compound:9090663|FD-Score:4.58|P-value:7.53E-7 ID:SGTC_3308|Compound:9128332|FD-Score:3.38|P-value:1.99E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.13|P-value:7.47E-6

Top fitness defect scores for YML028W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 8.17 63.6 µM 4.61E-18 4 1.54E-16
2 SGTC_457 grayanotoxin iii 8.10 135.0 µM 8.67E-18 10 2.74E-16
3 SGTC_2582 agaric acid 6.75 100.0 µM 6.93E-13 redox potentiating 138 7.62E-12
4 SGTC_1731 st035964 6.69 63.0 µM 1.09E-12 5 1.15E-11
5 SGTC_68 0986-0284 6.17 82.8 µM 4.57E-11 18 3.40E-10
6 SGTC_1110 3-hydroxy-3'-methoxyflavone 5.60 1.2 µM 2.01E-9 iron homeostasis 15 1.06E-8
7 SGTC_2328 6201408 5.50 200.0 µM 3.96E-9 4 1.95E-8
8 SGTC_1044 1222-0021 5.45 1.8 µM 5.33E-9 heme biosynthesis & mitochondrial translocase 18 2.56E-8
9 SGTC_2681 menadione 5.40 3.2 µM 7.04E-9 superoxide 15 3.29E-8
10 SGTC_2198 6589944 4.98 200.0 µM 8.75E-8 9 3.25E-7
11 SGTC_659 k018-0002 4.96 107.0 µM 9.78E-8 8 3.59E-7
12 SGTC_29 0299-0004 4.73 13.0 µM 3.37E-7 21 1.11E-6
13 SGTC_694 1315-0438 4.71 43.2 µM 3.82E-7 5 1.24E-6
14 SGTC_2368 9071271 4.58 200.0 µM 7.47E-7 16 2.28E-6
15 SGTC_2968 9090663 4.58 33.4 µM 7.53E-7 18 2.30E-6
16 SGTC_1422 4031-0024 4.15 15.5 µM 6.60E-6 iron homeostasis 14 1.65E-5
17 SGTC_2781 5567276 4.13 71.4 µM 7.23E-6 41 1.80E-5
18 SGTC_3323 9138564 4.13 53.9 µM 7.47E-6 17 1.85E-5
19 SGTC_2256 7954252 3.98 200.0 µM 1.45E-5 mitochondrial processes 8 3.38E-5
20 SGTC_1078 idebenone 3.97 36.1 µM 1.58E-5 Golgi 42 3.66E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.206 1.19E-33 YDR271C_d YDR271C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
0.183 1.33E-26 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
0.180 7.12E-26 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.165 7.95E-22 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.162 3.09E-21 YNL259C ATX1 hom Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake
0.162 3.45E-21 YDR269C_d YDR269C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.155 2.12E-19 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.147 8.93E-18 YNL241C ZWF1 hom Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
0.143 7.49E-17 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.139 5.42E-16 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.139 6.18E-16 YJL135W_d YJL135W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.138 9.91E-16 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.135 3.92E-15 YMR116C ASC1 hom G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
0.135 4.73E-15 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.130 3.35E-14 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels