HIPHOP chemogenomics database

YML041C / VPS71

Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting

Zygosity: Homozygous strain

fixedexpanded
Profile for YML041C / VPS71

Click on Significant Values for Screen Details

Top fitness defect scores for YML041C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2174	5790901	29.60	200.0 µM	4.85E-124	tubulin folding & SWR complex	1	3.74E-193
2	SGTC_2319	6033012	28.90	152.6 µM	8.41E-118	tubulin folding & SWR complex	1	2.33E-183
3	SGTC_221	trifluoperazine	27.00	10.3 µM	1.70E-103	NEO1-PIK1	3	6.57E-161
4	SGTC_1326	1431-2094	21.50	10.8 µM	1.40E-66	tubulin folding & SWR complex	1	5.36E-103
5	SGTC_229	benomyl	20.70	22.9 µM	1.52E-61	tubulin folding & SWR complex	3	4.17E-95
6	SGTC_2200	6600604	20.00	21.9 µM	4.13E-58	tubulin folding & SWR complex	1	1.01E-89
7	SGTC_673	1082-0474	19.10	110.0 µM	6.64E-53	RSC complex & mRNA processing	1	1.45E-81
8	SGTC_2352	9027995	18.10	87.8 µM	1.08E-47	tubulin folding & SWR complex	1	2.11E-73
9	SGTC_165	k038-0023	16.80	64.4 µM	1.87E-41	ubiquinone biosynthesis & proteasome	1	1.24E-63
10	SGTC_210	3551-0445	16.10	56.8 µM	2.74E-38		7	1.12E-58
11	SGTC_137	0438-0332	15.20	85.4 µM	3.76E-34	ubiquinone biosynthesis & proteasome	14	3.33E-52
12	SGTC_1433	4060-0023	15.10	7.6 µM	4.27E-34	tubulin folding & SWR complex	1	4.06E-52
13	SGTC_36	0335-0881	14.40	14.2 µM	3.35E-31	ubiquinone biosynthesis & proteasome	12	1.37E-47
14	SGTC_2141	5621638	13.90	7.7 µM	3.95E-29	tubulin folding & SWR complex	3	2.37E-44
15	SGTC_1643	st009866	13.50	20.2 µM	1.32E-27		6	5.68E-42
16	SGTC_2997	9067075	13.30	71.4 µM	7.67E-27	unfolded protein response	4	8.92E-41
17	SGTC_1401	3807-4679	12.60	7.6 µM	2.85E-24	tubulin folding & SWR complex	2	9.22E-37
18	SGTC_200	k038-0079	11.70	37.5 µM	4.67E-21	ubiquinone biosynthesis & proteasome	3	9.59E-32
19	SGTC_825	1988-2224	11.70	89.6 µM	4.90E-21	cell wall signaling	3	1.03E-31
20	SGTC_1875	nocodazole	11.40	6.0 µM	2.83E-20	tubulin folding & SWR complex	11	1.60E-30

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.717	0	YLR085C	ARP6	hom	Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.680	0	YAL011W	SWC3	hom	Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
0.663	0	YDR334W	SWR1	hom	Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.633	0	YDR485C	VPS72	hom	Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.592	3.56E-316	YBR231C	SWC5	hom	Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
0.517	1.09E-228	YER007W	PAC2	hom	Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.494	4.56E-205	YML124C	TUB3	hom	Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.437	1.89E-156	YOR349W	CIN1	hom	Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.408	2.13E-134	YOL012C	HTZ1	hom	Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
0.390	2.76E-122	YPL241C	CIN2	hom	GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.321	2.72E-81	YJL168C	SET2	hom	Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.311	3.68E-76	YNL107W	YAF9	hom	Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain
0.303	4.80E-72	YKL025C	PAN3	hom	Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.287	1.14E-64	YGL094C	PAN2	hom	Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.277	6.99E-60	YOR254C	SEC63	het	Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER