YML049C / RSE1

Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML049C / RSE1

Click on Significant Values for Screen Details ID:SGTC_95|Compound:0471-0001|FD-Score:3.61|P-value:2.45E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.49|P-value:3.65E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:3.57|P-value:2.82E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.66|P-value:2.00E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.23|P-value:8.95E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.39|P-value:5.28E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.39|P-value:1.52E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:5.05|P-value:5.55E-7 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.05|P-value:4.54E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.37|P-value:6.86E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:-5.07|P-value:7.09E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.67|P-value:1.93E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.33|P-value:6.32E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.32|P-value:8.13E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:-4.39|P-value:1.48E-5 ID:SGTC_1234|Compound:0326-0494|FD-Score:-3.58|P-value:3.44E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-4.16|P-value:3.88E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.24|P-value:8.64E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:-3.72|P-value:2.03E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.56|P-value:2.92E-4 ID:SGTC_1613|Compound:st002381|FD-Score:3.28|P-value:7.47E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-4.01|P-value:6.90E-5 ID:SGTC_1637|Compound:st007924|FD-Score:3.45|P-value:4.25E-4 ID:SGTC_1640|Compound:st009078|FD-Score:-3.53|P-value:4.09E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.38|P-value:5.46E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.76|P-value:1.41E-4 ID:SGTC_2117|Compound:5521686|FD-Score:3.45|P-value:4.21E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.84|P-value:1.34E-4 ID:SGTC_2163|Compound:5671233|FD-Score:4.46|P-value:8.22E-6 ID:SGTC_2281|Compound:7943571|FD-Score:3.42|P-value:4.69E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.58|P-value:3.40E-4 ID:SGTC_2458|Compound:5808907|FD-Score:-3.73|P-value:1.98E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.89|P-value:6.96E-9 ID:SGTC_2630|Compound:antimycin a|FD-Score:4.01|P-value:5.35E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.80|P-value:1.21E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.71|P-value:2.12E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:5.33|P-value:1.38E-7 ID:SGTC_2777|Compound:climbazole|FD-Score:3.51|P-value:3.48E-4 ID:SGTC_2780|Compound:5215298|FD-Score:-3.47|P-value:5.00E-4 ID:SGTC_2786|Compound:7743617|FD-Score:3.32|P-value:6.64E-4 ID:SGTC_2802|Compound:7925815|FD-Score:6.37|P-value:4.19E-10 ID:SGTC_2810|Compound:7959871|FD-Score:3.27|P-value:7.75E-4 ID:SGTC_2845|Compound:9003024|FD-Score:4.80|P-value:1.83E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.50|P-value:3.53E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.35|P-value:6.00E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.30|P-value:6.98E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.87|P-value:9.22E-5 ID:SGTC_3189|Compound:9110682|FD-Score:-4.91|P-value:1.47E-6 ID:SGTC_3335|Compound:9143315|FD-Score:5.97|P-value:4.52E-9 ID:SGTC_95|Compound:0471-0001|FD-Score:3.61|P-value:2.45E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.49|P-value:3.65E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:3.57|P-value:2.82E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.66|P-value:2.00E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.23|P-value:8.95E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.39|P-value:5.28E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.39|P-value:1.52E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:5.05|P-value:5.55E-7 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.05|P-value:4.54E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.37|P-value:6.86E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:-5.07|P-value:7.09E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.67|P-value:1.93E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.33|P-value:6.32E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.32|P-value:8.13E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:-4.39|P-value:1.48E-5 ID:SGTC_1234|Compound:0326-0494|FD-Score:-3.58|P-value:3.44E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-4.16|P-value:3.88E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.24|P-value:8.64E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:-3.72|P-value:2.03E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.56|P-value:2.92E-4 ID:SGTC_1613|Compound:st002381|FD-Score:3.28|P-value:7.47E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-4.01|P-value:6.90E-5 ID:SGTC_1637|Compound:st007924|FD-Score:3.45|P-value:4.25E-4 ID:SGTC_1640|Compound:st009078|FD-Score:-3.53|P-value:4.09E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.38|P-value:5.46E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.76|P-value:1.41E-4 ID:SGTC_2117|Compound:5521686|FD-Score:3.45|P-value:4.21E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.84|P-value:1.34E-4 ID:SGTC_2163|Compound:5671233|FD-Score:4.46|P-value:8.22E-6 ID:SGTC_2281|Compound:7943571|FD-Score:3.42|P-value:4.69E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.58|P-value:3.40E-4 ID:SGTC_2458|Compound:5808907|FD-Score:-3.73|P-value:1.98E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.89|P-value:6.96E-9 ID:SGTC_2630|Compound:antimycin a|FD-Score:4.01|P-value:5.35E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.80|P-value:1.21E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-3.71|P-value:2.12E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:5.33|P-value:1.38E-7 ID:SGTC_2777|Compound:climbazole|FD-Score:3.51|P-value:3.48E-4 ID:SGTC_2780|Compound:5215298|FD-Score:-3.47|P-value:5.00E-4 ID:SGTC_2786|Compound:7743617|FD-Score:3.32|P-value:6.64E-4 ID:SGTC_2802|Compound:7925815|FD-Score:6.37|P-value:4.19E-10 ID:SGTC_2810|Compound:7959871|FD-Score:3.27|P-value:7.75E-4 ID:SGTC_2845|Compound:9003024|FD-Score:4.80|P-value:1.83E-6 ID:SGTC_2933|Compound:9026794|FD-Score:3.50|P-value:3.53E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.35|P-value:6.00E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.30|P-value:6.98E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.87|P-value:9.22E-5 ID:SGTC_3189|Compound:9110682|FD-Score:-4.91|P-value:1.47E-6 ID:SGTC_3335|Compound:9143315|FD-Score:5.97|P-value:4.52E-9

Top fitness defect scores for YML049C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2802 7925815 6.37 54.5 µM 4.19E-10 1 9.25E-11 0.94
2 SGTC_3335 9143315 5.97 29.6 µM 4.52E-9 fatty acid desaturase (OLE1) 1 1.21E-9 0.95
3 SGTC_2567 aureobasidin a 5.89 60.0 nM 6.96E-9 18 1.93E-9 0.05
4 SGTC_2776 terbinafine 5.33 19.9 µM 1.38E-7 cell wall signaling 5 4.89E-8 0.38
5 SGTC_474 dl-dihydrosphingosine 5.05 5.0 µM 5.55E-7 2 2.19E-7 0.72
6 SGTC_2845 9003024 4.80 43.0 µM 1.83E-6 amide catabolism 6 7.96E-7 0.00
7 SGTC_2163 5671233 4.46 125.0 µM 8.22E-6 9 4.02E-6 0.12
8 SGTC_1019 4262-0212 4.05 3.4 µM 4.54E-5 ergosterol depletion effects on membrane 13 2.53E-5 0.04
9 SGTC_2630 antimycin a 4.01 100.0 µM 5.35E-5 mitochondrial processes 9 3.03E-5 0.05
10 SGTC_3153 9100258 3.87 49.5 µM 9.22E-5 7 5.44E-5 0.44
11 SGTC_2663 amphotericin b 3.80 100.0 µM 1.21E-4 5 7.27E-5 0.05
12 SGTC_2008 4012253 3.76 172.0 µM 1.41E-4 14 8.59E-5 0.03
13 SGTC_1159 4092-0538 3.67 61.2 µM 1.93E-4 6 1.20E-4 0.01
14 SGTC_158 k038-0087 3.66 26.4 µM 2.00E-4 ubiquinone biosynthesis & proteasome 34 1.25E-4 0.00
15 SGTC_95 0471-0001 3.61 561.1 µM 2.45E-4 7 1.55E-4 0.17
16 SGTC_155 k072-0202 3.57 43.6 µM 2.82E-4 10 1.81E-4 0.12
17 SGTC_1532 4',5'-dihydroxyflavone 3.56 78.7 µM 2.92E-4 Golgi 11 1.88E-4 0.01
18 SGTC_2777 climbazole 3.51 1.5 µM 3.48E-4 iron homeostasis 7 2.27E-4 0.04
19 SGTC_2933 9026794 3.50 32.5 µM 3.53E-4 7 2.30E-4 0.03
20 SGTC_120 rhodamine 6G 3.49 52.3 µM 3.65E-4 mitochondrial processes 22 2.39E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 7.78E-24 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.107 5.10E-10 YFR032C-B_p YFR032C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.104 1.81E-9 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.100 6.54E-9 YGR175C ERG1 het Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
0.091 1.24E-7 YKL074C MUD2 hom Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
0.091 1.50E-7 YBR110W ALG1 het Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
0.084 1.20E-6 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.083 1.61E-6 YER175C TMT1 hom Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
0.081 2.65E-6 YPR004C AIM45 hom Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response
0.081 3.04E-6 YLR285W NNT1 hom S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination
0.081 2.97E-6 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.077 7.92E-6 YBR196C PGI1 het Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
0.076 9.22E-6 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.076 9.22E-6 YBL054W TOD6 hom PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication
0.075 1.23E-5 YOR029W_d YOR029W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data