YML061C / PIF1

DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells

Zygosity: Homozygous strain
fixedexpanded
Profile for YML061C / PIF1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:3.60|P-value:5.44E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.62|P-value:5.07E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:-5.00|P-value:2.33E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:5.05|P-value:2.21E-6 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.84|P-value:2.43E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.93|P-value:1.81E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-6.72|P-value:3.94E-10 ID:SGTC_173|Compound:1273-0061|FD-Score:9.50|P-value:2.59E-18 ID:SGTC_236|Compound:1509-0011|FD-Score:12.20|P-value:4.07E-29 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.37|P-value:3.64E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:11.80|P-value:1.95E-27 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.15|P-value:7.28E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.69|P-value:8.77E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.62|P-value:1.37E-7 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.99|P-value:1.45E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.79|P-value:2.61E-4 ID:SGTC_487|Compound:nimodipine|FD-Score:3.50|P-value:7.38E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-3.52|P-value:6.22E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:3.72|P-value:3.69E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.86|P-value:2.29E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.84|P-value:2.49E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.02|P-value:1.29E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.78|P-value:7.00E-6 ID:SGTC_830|Compound:4076-0286|FD-Score:4.70|P-value:9.61E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:4.82|P-value:5.97E-6 ID:SGTC_832|Compound:4083-0019|FD-Score:4.96|P-value:3.35E-6 ID:SGTC_851|Compound:0394-0702|FD-Score:4.29|P-value:4.97E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:4.17|P-value:7.65E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:4.73|P-value:8.56E-6 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.53|P-value:1.95E-5 ID:SGTC_1064|Compound:honokiol|FD-Score:4.21|P-value:6.62E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.29|P-value:4.95E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.48|P-value:7.96E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.45|P-value:8.68E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:-3.57|P-value:5.35E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.46|P-value:7.57E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:-4.03|P-value:1.10E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:5.54|P-value:2.33E-7 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.70|P-value:3.89E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:5.21|P-value:1.11E-6 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.40|P-value:9.21E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.10|P-value:9.87E-5 ID:SGTC_1627|Compound:st003713|FD-Score:3.79|P-value:2.88E-4 ID:SGTC_1656|Compound:st012921|FD-Score:-3.40|P-value:9.21E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.98|P-value:1.32E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.27|P-value:4.57E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.31|P-value:5.98E-7 ID:SGTC_1742|Compound:st038235|FD-Score:5.36|P-value:5.57E-7 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.36|P-value:3.32E-5 ID:SGTC_1792|Compound:st045773|FD-Score:4.17|P-value:7.73E-5 ID:SGTC_1814|Compound:st052110|FD-Score:-4.10|P-value:8.63E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-3.94|P-value:1.55E-4 ID:SGTC_1882|Compound:5615643|FD-Score:3.55|P-value:6.35E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.41|P-value:8.87E-4 ID:SGTC_1962|Compound:st076595|FD-Score:-5.22|P-value:8.90E-7 ID:SGTC_1968|Compound:st069590|FD-Score:-3.39|P-value:9.47E-4 ID:SGTC_1987|Compound:st072290|FD-Score:3.56|P-value:6.27E-4 ID:SGTC_1994|Compound:st072947|FD-Score:-4.47|P-value:2.16E-5 ID:SGTC_2021|Compound:4019055|FD-Score:-3.55|P-value:5.78E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.73|P-value:3.59E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.71|P-value:3.86E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.94|P-value:1.75E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:6.89|P-value:1.88E-10 ID:SGTC_2329|Compound:6138490|FD-Score:9.32|P-value:1.05E-17 ID:SGTC_2331|Compound:7980721|FD-Score:4.85|P-value:5.25E-6 ID:SGTC_2445|Compound:5742597|FD-Score:4.00|P-value:1.39E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.98|P-value:1.35E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.50|P-value:1.71E-9 ID:SGTC_2777|Compound:climbazole|FD-Score:-6.02|P-value:1.82E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.62|P-value:4.47E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-4.03|P-value:1.13E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-5.47|P-value:2.85E-7 ID:SGTC_2896|Compound:9052656|FD-Score:-6.90|P-value:1.42E-10 ID:SGTC_2942|Compound:9052777|FD-Score:-3.77|P-value:2.78E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.95|P-value:3.50E-6 ID:SGTC_3220|Compound:9131269|FD-Score:-3.92|P-value:1.63E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.41|P-value:2.68E-5 ID:SGTC_10|Compound:0279-0212|FD-Score:3.60|P-value:5.44E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.62|P-value:5.07E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:-5.00|P-value:2.33E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:5.05|P-value:2.21E-6 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.84|P-value:2.43E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.93|P-value:1.81E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-6.72|P-value:3.94E-10 ID:SGTC_173|Compound:1273-0061|FD-Score:9.50|P-value:2.59E-18 ID:SGTC_236|Compound:1509-0011|FD-Score:12.20|P-value:4.07E-29 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.37|P-value:3.64E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:11.80|P-value:1.95E-27 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.15|P-value:7.28E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.69|P-value:8.77E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.62|P-value:1.37E-7 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.99|P-value:1.45E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.79|P-value:2.61E-4 ID:SGTC_487|Compound:nimodipine|FD-Score:3.50|P-value:7.38E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-3.52|P-value:6.22E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:3.72|P-value:3.69E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.86|P-value:2.29E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.84|P-value:2.49E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.02|P-value:1.29E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.78|P-value:7.00E-6 ID:SGTC_830|Compound:4076-0286|FD-Score:4.70|P-value:9.61E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:4.82|P-value:5.97E-6 ID:SGTC_832|Compound:4083-0019|FD-Score:4.96|P-value:3.35E-6 ID:SGTC_851|Compound:0394-0702|FD-Score:4.29|P-value:4.97E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:4.17|P-value:7.65E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:4.73|P-value:8.56E-6 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.53|P-value:1.95E-5 ID:SGTC_1064|Compound:honokiol|FD-Score:4.21|P-value:6.62E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.29|P-value:4.95E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.48|P-value:7.96E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.45|P-value:8.68E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:-3.57|P-value:5.35E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.46|P-value:7.57E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:-4.03|P-value:1.10E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:5.54|P-value:2.33E-7 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.70|P-value:3.89E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:5.21|P-value:1.11E-6 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.40|P-value:9.21E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.10|P-value:9.87E-5 ID:SGTC_1627|Compound:st003713|FD-Score:3.79|P-value:2.88E-4 ID:SGTC_1656|Compound:st012921|FD-Score:-3.40|P-value:9.21E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.98|P-value:1.32E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.27|P-value:4.57E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.31|P-value:5.98E-7 ID:SGTC_1742|Compound:st038235|FD-Score:5.36|P-value:5.57E-7 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.36|P-value:3.32E-5 ID:SGTC_1792|Compound:st045773|FD-Score:4.17|P-value:7.73E-5 ID:SGTC_1814|Compound:st052110|FD-Score:-4.10|P-value:8.63E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-3.94|P-value:1.55E-4 ID:SGTC_1882|Compound:5615643|FD-Score:3.55|P-value:6.35E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.41|P-value:8.87E-4 ID:SGTC_1962|Compound:st076595|FD-Score:-5.22|P-value:8.90E-7 ID:SGTC_1968|Compound:st069590|FD-Score:-3.39|P-value:9.47E-4 ID:SGTC_1987|Compound:st072290|FD-Score:3.56|P-value:6.27E-4 ID:SGTC_1994|Compound:st072947|FD-Score:-4.47|P-value:2.16E-5 ID:SGTC_2021|Compound:4019055|FD-Score:-3.55|P-value:5.78E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.73|P-value:3.59E-4 ID:SGTC_2177|Compound:5811945|FD-Score:3.71|P-value:3.86E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.94|P-value:1.75E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:6.89|P-value:1.88E-10 ID:SGTC_2329|Compound:6138490|FD-Score:9.32|P-value:1.05E-17 ID:SGTC_2331|Compound:7980721|FD-Score:4.85|P-value:5.25E-6 ID:SGTC_2445|Compound:5742597|FD-Score:4.00|P-value:1.39E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.98|P-value:1.35E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.50|P-value:1.71E-9 ID:SGTC_2777|Compound:climbazole|FD-Score:-6.02|P-value:1.82E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.62|P-value:4.47E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-4.03|P-value:1.13E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-5.47|P-value:2.85E-7 ID:SGTC_2896|Compound:9052656|FD-Score:-6.90|P-value:1.42E-10 ID:SGTC_2942|Compound:9052777|FD-Score:-3.77|P-value:2.78E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.95|P-value:3.50E-6 ID:SGTC_3220|Compound:9131269|FD-Score:-3.92|P-value:1.63E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.41|P-value:2.68E-5

Top fitness defect scores for YML061C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 12.20 5.3 µM 4.07E-29 146 1.05E-34
2 SGTC_310 1273-0058 11.80 1.1 µM 1.95E-27 mitochondrial stress 24 1.10E-32
3 SGTC_173 1273-0061 9.50 2.7 µM 2.59E-18 mitochondrial stress 46 1.03E-21
4 SGTC_2329 6138490 9.32 200.0 µM 1.05E-17 3 5.56E-21
5 SGTC_2250 alverine citrate 6.89 130.0 µM 1.88E-10 36 2.86E-12
6 SGTC_2688 kanamycin b 6.50 96.7 µM 1.71E-9 calcium & mitochondrial duress 132 4.04E-11
7 SGTC_1332 1480-0653 5.54 17.5 µM 2.33E-7 TSC3-RPN4 4 1.48E-8
8 SGTC_1742 st038235 5.36 28.9 µM 5.57E-7 mitochondrial stress 64 4.20E-8
9 SGTC_1461 k072-0203 5.21 53.8 µM 1.11E-6 1 9.56E-8
10 SGTC_118 0083-0097 5.05 135.3 µM 2.21E-6 translation 52 2.19E-7
11 SGTC_832 4083-0019 4.96 164.0 µM 3.35E-6 translation 42 3.61E-7
12 SGTC_3218 9131285 4.95 49.5 µM 3.50E-6 endomembrane recycling 17 3.80E-7
13 SGTC_2331 7980721 4.85 200.0 µM 5.25E-6 7 6.17E-7
14 SGTC_831 k061-1017 4.82 217.0 µM 5.97E-6 translation 26 7.20E-7
15 SGTC_819 1013-0258 4.78 27.7 µM 7.00E-6 22 8.72E-7
16 SGTC_969 1313-0238 4.73 134.0 µM 8.56E-6 mitochondrial processes 11 1.11E-6
17 SGTC_830 4076-0286 4.70 57.8 µM 9.61E-6 translation 38 1.27E-6
18 SGTC_1036 k216-0215 4.53 33.5 µM 1.95E-5 mitochondrial response to ROS 34 2.97E-6
19 SGTC_275 trichlorophene 4.37 5.7 µM 3.64E-5 mitochondrial stress 206 6.27E-6
20 SGTC_1066 ketoconazole 4.29 628.0 nM 4.95E-5 13 9.07E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.490 2.16E-202 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.490 1.43E-201 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit
0.480 4.88E-193 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.476 2.74E-189 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.466 1.24E-179 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.458 3.13E-173 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.457 1.97E-172 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.455 7.45E-171 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.443 6.05E-161 YER077C_p YER077C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.442 3.19E-160 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.441 1.14E-159 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.441 1.21E-159 YPL183W-A RTC6 hom Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
0.435 3.91E-155 YNL184C_p YNL184C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.435 4.15E-155 YMR282C AEP2 hom Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
0.433 1.25E-152 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene