YML069W / POB3

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML069W / POB3

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:2.97|P-value:8.29E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.07|P-value:6.10E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.02|P-value:7.09E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.28|P-value:3.07E-6 ID:SGTC_155|Compound:k072-0202|FD-Score:3.08|P-value:5.61E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.05|P-value:6.47E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.19|P-value:3.68E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.00|P-value:7.62E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:4.56|P-value:7.29E-7 ID:SGTC_664|Compound:1222-0054|FD-Score:-2.98|P-value:8.41E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:4.25|P-value:3.47E-6 ID:SGTC_859|Compound:0439-0102|FD-Score:3.06|P-value:6.07E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.19|P-value:3.72E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.47|P-value:1.24E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:4.08|P-value:8.07E-6 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.28|P-value:2.67E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.18|P-value:5.06E-6 ID:SGTC_1875|Compound:nocodazole|FD-Score:2.96|P-value:8.64E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.07|P-value:5.82E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.83|P-value:2.68E-5 ID:SGTC_2261|Compound:9010538|FD-Score:4.37|P-value:1.95E-6 ID:SGTC_2297|Compound:7971671|FD-Score:3.31|P-value:2.33E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.05|P-value:6.37E-4 ID:SGTC_2332|Compound:7982454|FD-Score:4.45|P-value:1.28E-6 ID:SGTC_2335|Compound:9018011|FD-Score:6.04|P-value:8.52E-11 ID:SGTC_2338|Compound:7999974|FD-Score:7.92|P-value:2.85E-17 ID:SGTC_2340|Compound:7987427|FD-Score:5.27|P-value:1.28E-8 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.14|P-value:4.69E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.12|P-value:4.92E-4 ID:SGTC_2809|Compound:7964133|FD-Score:3.08|P-value:5.68E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.09|P-value:5.39E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.15|P-value:5.98E-6 ID:SGTC_3212|Compound:9128307|FD-Score:6.09|P-value:6.12E-11 ID:SGTC_3302|Compound:9125395|FD-Score:3.11|P-value:4.96E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:2.97|P-value:8.29E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.07|P-value:6.10E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.02|P-value:7.09E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.28|P-value:3.07E-6 ID:SGTC_155|Compound:k072-0202|FD-Score:3.08|P-value:5.61E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.05|P-value:6.47E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.19|P-value:3.68E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.00|P-value:7.62E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:4.56|P-value:7.29E-7 ID:SGTC_664|Compound:1222-0054|FD-Score:-2.98|P-value:8.41E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:4.25|P-value:3.47E-6 ID:SGTC_859|Compound:0439-0102|FD-Score:3.06|P-value:6.07E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.19|P-value:3.72E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.47|P-value:1.24E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:4.08|P-value:8.07E-6 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.28|P-value:2.67E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.18|P-value:5.06E-6 ID:SGTC_1875|Compound:nocodazole|FD-Score:2.96|P-value:8.64E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.07|P-value:5.82E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.83|P-value:2.68E-5 ID:SGTC_2261|Compound:9010538|FD-Score:4.37|P-value:1.95E-6 ID:SGTC_2297|Compound:7971671|FD-Score:3.31|P-value:2.33E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.05|P-value:6.37E-4 ID:SGTC_2332|Compound:7982454|FD-Score:4.45|P-value:1.28E-6 ID:SGTC_2335|Compound:9018011|FD-Score:6.04|P-value:8.52E-11 ID:SGTC_2338|Compound:7999974|FD-Score:7.92|P-value:2.85E-17 ID:SGTC_2340|Compound:7987427|FD-Score:5.27|P-value:1.28E-8 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.14|P-value:4.69E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.12|P-value:4.92E-4 ID:SGTC_2809|Compound:7964133|FD-Score:3.08|P-value:5.68E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.09|P-value:5.39E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.15|P-value:5.98E-6 ID:SGTC_3212|Compound:9128307|FD-Score:6.09|P-value:6.12E-11 ID:SGTC_3302|Compound:9125395|FD-Score:3.11|P-value:4.96E-4

Top fitness defect scores for YML069W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2338 7999974 7.92 200.0 µM 2.85E-17 2 1.18E-15 2.30
2 SGTC_3212 9128307 6.09 49.5 µM 6.12E-11 2 5.68E-10 0.03
3 SGTC_2335 9018011 6.04 154.2 µM 8.52E-11 2 7.65E-10 0.38
4 SGTC_2340 7987427 5.27 183.4 µM 1.28E-8 2 6.91E-8 0.15
5 SGTC_645 0845-0943 4.56 5.4 µM 7.29E-7 60S ribosome export 4 2.61E-6 0.39
6 SGTC_2332 7982454 4.45 138.9 µM 1.28E-6 9 4.32E-6 0.30
7 SGTC_2261 9010538 4.37 184.7 µM 1.95E-6 2 6.33E-6 0.15
8 SGTC_706 4262-0057 4.25 46.9 µM 3.47E-6 fatty acid desaturase (OLE1) 8 1.06E-5 0.32
9 SGTC_1746 st038117 4.18 42.0 µM 5.06E-6 4 1.49E-5 0.13
10 SGTC_1136 pergolide 4.08 1.1 µM 8.07E-6 12 2.27E-5 0.04
11 SGTC_2297 7971671 3.31 152.3 µM 2.33E-4 RNA processing & uracil transport 13 4.69E-4 0.03
12 SGTC_373 0844-0104 3.19 25.9 µM 3.68E-4 ERAD & cell cycle 25 7.07E-4 0.20
13 SGTC_1010 4112-3296 3.19 15.2 µM 3.72E-4 17 7.14E-4 0.30
14 SGTC_2625 euphol 3.12 100.0 µM 4.92E-4 14 9.19E-4 0.05
15 SGTC_3302 9125395 3.11 16.4 µM 4.96E-4 12 9.25E-4 0.01
16 SGTC_2873 9040596 3.09 71.4 µM 5.39E-4 18 9.97E-4 0.01
17 SGTC_155 k072-0202 3.08 43.6 µM 5.61E-4 15 0.00103 0.07
18 SGTC_2809 7964133 3.08 13.0 µM 5.68E-4 60S ribosome export 20 0.00105 0.02
19 SGTC_2029 5175081 3.07 200.0 µM 5.82E-4 14 0.00107 0.06
20 SGTC_859 0439-0102 3.06 54.6 µM 6.07E-4 redox potentiating 22 0.00111 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.203 1.41E-32 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.184 6.24E-27 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.156 8.29E-20 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.148 8.55E-18 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.142 1.63E-16 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.138 8.19E-16 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.130 3.96E-14 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.129 7.44E-14 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.129 7.59E-14 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.124 5.03E-13 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.123 9.12E-13 YKL022C CDC16 het Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
0.122 1.57E-12 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.121 2.10E-12 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.119 4.22E-12 YDL039C PRM7 hom Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
0.118 7.16E-12 YJL173C RFA3 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress