YML072C / TCB3

Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact

Zygosity: Homozygous strain
fixedexpanded
Profile for YML072C / TCB3

Click on Significant Values for Screen Details ID:SGTC_103|Compound:0844-0104|FD-Score:4.47|P-value:8.92E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:9.22|P-value:4.95E-19 ID:SGTC_142|Compound:0335-0849|FD-Score:5.89|P-value:8.26E-9 ID:SGTC_160|Compound:3453-0671|FD-Score:5.78|P-value:1.51E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.94|P-value:1.14E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.86|P-value:1.12E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:4.89|P-value:1.36E-6 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.72|P-value:1.88E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:4.72|P-value:2.95E-6 ID:SGTC_504|Compound:lovastatin|FD-Score:3.35|P-value:6.49E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.87|P-value:1.08E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.59|P-value:5.38E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:3.28|P-value:8.25E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.67|P-value:2.13E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:4.00|P-value:6.06E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:4.32|P-value:1.69E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.82|P-value:1.24E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.29|P-value:1.92E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.59|P-value:2.89E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.23|P-value:9.64E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.32|P-value:7.23E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.74|P-value:1.63E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.32|P-value:7.09E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:4.87|P-value:1.47E-6 ID:SGTC_1458|Compound:k015-0034|FD-Score:4.09|P-value:4.30E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.12|P-value:4.10E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.56|P-value:5.13E-8 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.31|P-value:7.30E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-5.00|P-value:8.47E-7 ID:SGTC_1677|Compound:st019204|FD-Score:4.97|P-value:9.39E-7 ID:SGTC_1712|Compound:st032288|FD-Score:-4.27|P-value:2.25E-5 ID:SGTC_1750|Compound:st081859|FD-Score:20.90|P-value:5.97E-90 ID:SGTC_1772|Compound:st048439|FD-Score:3.59|P-value:2.89E-4 ID:SGTC_1775|Compound:st081588|FD-Score:3.46|P-value:4.53E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-4.33|P-value:1.76E-5 ID:SGTC_1973|Compound:st070275|FD-Score:3.22|P-value:9.97E-4 ID:SGTC_1982|Compound:st072467|FD-Score:7.48|P-value:3.79E-13 ID:SGTC_2070|Compound:5230947|FD-Score:3.78|P-value:1.44E-4 ID:SGTC_2073|Compound:5212005|FD-Score:-3.50|P-value:4.17E-4 ID:SGTC_2075|Compound:5212009|FD-Score:-7.13|P-value:4.45E-12 ID:SGTC_2128|Compound:5255449|FD-Score:-4.86|P-value:1.70E-6 ID:SGTC_2204|Compound:7233102|FD-Score:3.36|P-value:6.29E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.88|P-value:9.75E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.41|P-value:5.29E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.31|P-value:1.79E-5 ID:SGTC_2585|Compound:alliin|FD-Score:4.70|P-value:3.25E-6 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.35|P-value:6.46E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.41|P-value:5.23E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.36|P-value:6.36E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-3.37|P-value:6.38E-4 ID:SGTC_2971|Compound:9092186|FD-Score:4.17|P-value:3.08E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.60|P-value:2.77E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.32|P-value:7.25E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-3.28|P-value:8.57E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.63|P-value:2.45E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.47|P-value:8.92E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:9.22|P-value:4.95E-19 ID:SGTC_142|Compound:0335-0849|FD-Score:5.89|P-value:8.26E-9 ID:SGTC_160|Compound:3453-0671|FD-Score:5.78|P-value:1.51E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.94|P-value:1.14E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.86|P-value:1.12E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:4.89|P-value:1.36E-6 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.72|P-value:1.88E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:4.72|P-value:2.95E-6 ID:SGTC_504|Compound:lovastatin|FD-Score:3.35|P-value:6.49E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.87|P-value:1.08E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.59|P-value:5.38E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:3.28|P-value:8.25E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.67|P-value:2.13E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:4.00|P-value:6.06E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:4.32|P-value:1.69E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.82|P-value:1.24E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.29|P-value:1.92E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.59|P-value:2.89E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.23|P-value:9.64E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.32|P-value:7.23E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.74|P-value:1.63E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.32|P-value:7.09E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:4.87|P-value:1.47E-6 ID:SGTC_1458|Compound:k015-0034|FD-Score:4.09|P-value:4.30E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.12|P-value:4.10E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.56|P-value:5.13E-8 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.31|P-value:7.30E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-5.00|P-value:8.47E-7 ID:SGTC_1677|Compound:st019204|FD-Score:4.97|P-value:9.39E-7 ID:SGTC_1712|Compound:st032288|FD-Score:-4.27|P-value:2.25E-5 ID:SGTC_1750|Compound:st081859|FD-Score:20.90|P-value:5.97E-90 ID:SGTC_1772|Compound:st048439|FD-Score:3.59|P-value:2.89E-4 ID:SGTC_1775|Compound:st081588|FD-Score:3.46|P-value:4.53E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-4.33|P-value:1.76E-5 ID:SGTC_1973|Compound:st070275|FD-Score:3.22|P-value:9.97E-4 ID:SGTC_1982|Compound:st072467|FD-Score:7.48|P-value:3.79E-13 ID:SGTC_2070|Compound:5230947|FD-Score:3.78|P-value:1.44E-4 ID:SGTC_2073|Compound:5212005|FD-Score:-3.50|P-value:4.17E-4 ID:SGTC_2075|Compound:5212009|FD-Score:-7.13|P-value:4.45E-12 ID:SGTC_2128|Compound:5255449|FD-Score:-4.86|P-value:1.70E-6 ID:SGTC_2204|Compound:7233102|FD-Score:3.36|P-value:6.29E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.88|P-value:9.75E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.41|P-value:5.29E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.31|P-value:1.79E-5 ID:SGTC_2585|Compound:alliin|FD-Score:4.70|P-value:3.25E-6 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.35|P-value:6.46E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.41|P-value:5.23E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.36|P-value:6.36E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-3.37|P-value:6.38E-4 ID:SGTC_2971|Compound:9092186|FD-Score:4.17|P-value:3.08E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.60|P-value:2.77E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.32|P-value:7.25E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-3.28|P-value:8.57E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.63|P-value:2.45E-4

Top fitness defect scores for YML072C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 20.90 2.4 µM 5.97E-90 endomembrane recycling 11 9.49E-98
2 SGTC_137 0438-0332 9.22 85.4 µM 4.95E-19 ubiquinone biosynthesis & proteasome 26 1.54E-20
3 SGTC_1982 st072467 7.48 71.9 µM 3.79E-13 7 3.85E-14
4 SGTC_142 0335-0849 5.89 62.7 µM 8.26E-9 ubiquinone biosynthesis & proteasome 65 1.99E-9
5 SGTC_160 3453-0671 5.78 289.7 µM 1.51E-8 10 3.82E-9
6 SGTC_1677 st019204 4.97 53.9 µM 9.39E-7 4 3.39E-7
7 SGTC_340 k038-0059 4.89 454.0 µM 1.36E-6 ubiquinone biosynthesis & proteasome 31 5.07E-7
8 SGTC_1449 4112-4002 4.87 201.0 µM 1.47E-6 RPP1 & pyrimidine depletion 12 5.52E-7
9 SGTC_496 calpeptin 4.72 138.0 µM 2.95E-6 7 1.17E-6
10 SGTC_2585 alliin 4.70 100.0 µM 3.25E-6 12 1.30E-6
11 SGTC_568 1193-0097 4.59 7.5 µM 5.38E-6 ERAD & cell cycle 110 2.25E-6
12 SGTC_103 0844-0104 4.47 51.8 µM 8.92E-6 ERAD & cell cycle 121 3.89E-6
13 SGTC_801 0866-0028 4.32 10.1 µM 1.69E-5 15 7.80E-6
14 SGTC_2533 agelasine 4.31 13.7 µM 1.79E-5 ERG2 45 8.30E-6
15 SGTC_815 1582-0027 4.29 36.9 µM 1.92E-5 16 8.94E-6
16 SGTC_2971 9092186 4.17 9.9 µM 3.08E-5 ergosterol biosynthesis 24 1.49E-5
17 SGTC_1458 k015-0034 4.09 6.3 µM 4.30E-5 14 2.14E-5
18 SGTC_794 k038-0031 4.00 316.0 µM 6.06E-5 ubiquinone biosynthesis & proteasome 106 3.10E-5
19 SGTC_2412 st077135 3.88 33.4 µM 9.75E-5 26 5.20E-5
20 SGTC_806 3966-4455 3.82 5.9 µM 1.24E-4 24 6.72E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.455 5.29E-171 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.378 1.55E-114 YPR153W YPR153W hom Putative protein of unknown function
0.333 6.63E-88 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.290 4.06E-66 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.281 6.37E-62 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.276 1.15E-59 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.248 4.28E-48 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.245 6.17E-47 YBR255W MTC4 hom Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
0.232 4.38E-42 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.208 2.90E-34 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.198 6.63E-31 YKL207W EMC3 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111
0.193 2.14E-29 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.186 1.90E-27 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.186 2.23E-27 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.181 3.00E-26 YJR129C_p YJR129C_p hom Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm