HIPHOP chemogenomics database

YML092C / PRE8

Alpha 2 subunit of the 20S proteasome

Zygosity: Heterozygous strain

fixedexpanded
Profile for YML092C / PRE8

Click on Significant Values for Screen Details

Top fitness defect scores for YML092C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_64	000l-0018	13.20	272.1 µM	1.67E-28	ubiquinone biosynthesis & proteasome	9	7.67E-40	2.27
2	SGTC_1597	7-benzyloxygramine	11.40	47.5 µM	5.57E-22	calcium & mitochondrial duress	14	1.29E-30	0.68
3	SGTC_2277	7947813	10.40	106.9 µM	2.47E-18	RSC complex & mRNA processing	2	1.86E-25	1.91
4	SGTC_1880	MG-132	10.10	525.0 µM	1.50E-17		3	2.37E-24	2.34
5	SGTC_2250	alverine citrate	9.60	130.0 µM	5.66E-16		9	4.04E-22	0.46
6	SGTC_1712	st032288	9.55	53.4 µM	7.68E-16	calcium & mitochondrial duress	3	6.23E-22	0.41
7	SGTC_187	1488-2104	8.46	32.7 µM	8.63E-13	calcium & mitochondrial duress	1	1.29E-17	1.51
8	SGTC_2460	5552655	7.27	174.2 µM	7.43E-10	amide catabolism	1	1.84E-13	1.64
9	SGTC_137	0438-0332	6.96	85.4 µM	3.53E-9	ubiquinone biosynthesis & proteasome	16	1.67E-12	0.12
10	SGTC_201	4262-0159	6.84	12.8 µM	6.60E-9	ergosterol depletion effects on membrane	5	4.04E-12	0.75
11	SGTC_1919	st067113	6.62	52.3 µM	1.93E-8	TSC3-RPN4	2	1.85E-11	1.08
12	SGTC_200	k038-0079	6.54	37.5 µM	2.78E-8	ubiquinone biosynthesis & proteasome	4	3.09E-11	1.25
13	SGTC_635	k038-0028	6.26	24.7 µM	1.03E-7	ubiquinone biosynthesis & proteasome	5	1.96E-10	0.69
14	SGTC_2268	7933382	5.99	153.8 µM	3.37E-7		3	1.05E-9	0.31
15	SGTC_388	3',4'-dichloroisocoumarin	5.84	16.7 µM	6.48E-7		2	2.65E-9	0.85
16	SGTC_2279	7948423	5.61	32.9 µM	1.68E-6		1	1.02E-8	0.59
17	SGTC_158	k038-0087	5.59	26.4 µM	1.79E-6	ubiquinone biosynthesis & proteasome	15	1.11E-8	0.07
18	SGTC_216	k038-0035	5.49	28.4 µM	2.74E-6	ubiquinone biosynthesis & proteasome	7	2.04E-8	0.02
19	SGTC_253	dyclonine	5.44	31.2 µM	3.36E-6	amide catabolism	1	2.72E-8	0.86
20	SGTC_165	k038-0023	5.36	64.4 µM	4.54E-6	ubiquinone biosynthesis & proteasome	10	4.16E-8	0.03

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.493	8.04E-205	YMR314W	PRE5	het	Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.468	9.67E-182	YER094C	PUP3	het	Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.423	2.10E-145	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.415	1.79E-139	YOR362C	PRE10	het	Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.414	7.32E-139	YER012W	PRE1	het	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.409	2.96E-135	YGL011C	SCL1	het	Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.381	3.36E-116	YOL038W	PRE6	het	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.337	1.43E-89	YGR253C	PUP2	het	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.329	5.67E-85	YBL041W	PRE7	het	Beta 6 subunit of the 20S proteasome
0.273	3.49E-58	YJL001W	PRE3	het	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.267	1.08E-55	YPR103W	PRE2	het	Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.257	9.28E-52	YLR021W	IRC25	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.241	2.24E-45	YNL111C	CYB5	hom	Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.234	5.84E-43	YDR086C	SSS1	het	Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.229	4.20E-41	YJL165C	HAL5	hom	Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication