YML100W / TSL1

Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YML100W / TSL1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.19|P-value:1.65E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.04|P-value:3.05E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.02|P-value:3.30E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.71|P-value:2.06E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.03|P-value:4.17E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:-3.60|P-value:3.42E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:3.49|P-value:4.24E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.93|P-value:6.94E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.74|P-value:1.78E-5 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.04|P-value:3.89E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.19|P-value:2.14E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.74|P-value:9.90E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-3.64|P-value:2.85E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-2.93|P-value:6.11E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.06|P-value:3.65E-4 ID:SGTC_1612|Compound:st002233|FD-Score:-3.53|P-value:4.74E-5 ID:SGTC_1615|Compound:st002656|FD-Score:-2.83|P-value:9.00E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.28|P-value:1.09E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.79|P-value:3.68E-8 ID:SGTC_1779|Compound:st039427|FD-Score:2.79|P-value:8.26E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.99|P-value:4.82E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.53|P-value:3.63E-5 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-3.26|P-value:1.60E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.64|P-value:2.17E-5 ID:SGTC_2294|Compound:7971431|FD-Score:4.20|P-value:1.17E-6 ID:SGTC_2296|Compound:7971656|FD-Score:-6.25|P-value:2.02E-12 ID:SGTC_2301|Compound:7769476|FD-Score:-5.77|P-value:7.83E-11 ID:SGTC_2363|Compound:9053361|FD-Score:4.96|P-value:1.32E-8 ID:SGTC_2400|Compound:5203494|FD-Score:-3.52|P-value:4.95E-5 ID:SGTC_2415|Compound:st077333|FD-Score:3.14|P-value:2.04E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.30|P-value:1.33E-9 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.05|P-value:3.79E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.90|P-value:6.95E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.11|P-value:2.27E-4 ID:SGTC_2941|Compound:9056145|FD-Score:3.14|P-value:2.04E-4 ID:SGTC_2967|Compound:9089538|FD-Score:4.19|P-value:1.27E-6 ID:SGTC_2973|Compound:9094665|FD-Score:3.61|P-value:2.40E-5 ID:SGTC_3096|Compound:9117468|FD-Score:3.02|P-value:3.38E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-2.91|P-value:6.59E-4 ID:SGTC_3162|Compound:9099653|FD-Score:4.06|P-value:2.59E-6 ID:SGTC_6|Compound:0844-0013|FD-Score:3.19|P-value:1.65E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.04|P-value:3.05E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.02|P-value:3.30E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.71|P-value:2.06E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.03|P-value:4.17E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:-3.60|P-value:3.42E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:3.49|P-value:4.24E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.93|P-value:6.94E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.74|P-value:1.78E-5 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.04|P-value:3.89E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.19|P-value:2.14E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.74|P-value:9.90E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-3.64|P-value:2.85E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-2.93|P-value:6.11E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.06|P-value:3.65E-4 ID:SGTC_1612|Compound:st002233|FD-Score:-3.53|P-value:4.74E-5 ID:SGTC_1615|Compound:st002656|FD-Score:-2.83|P-value:9.00E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.28|P-value:1.09E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.79|P-value:3.68E-8 ID:SGTC_1779|Compound:st039427|FD-Score:2.79|P-value:8.26E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.99|P-value:4.82E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.53|P-value:3.63E-5 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-3.26|P-value:1.60E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.64|P-value:2.17E-5 ID:SGTC_2294|Compound:7971431|FD-Score:4.20|P-value:1.17E-6 ID:SGTC_2296|Compound:7971656|FD-Score:-6.25|P-value:2.02E-12 ID:SGTC_2301|Compound:7769476|FD-Score:-5.77|P-value:7.83E-11 ID:SGTC_2363|Compound:9053361|FD-Score:4.96|P-value:1.32E-8 ID:SGTC_2400|Compound:5203494|FD-Score:-3.52|P-value:4.95E-5 ID:SGTC_2415|Compound:st077333|FD-Score:3.14|P-value:2.04E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.30|P-value:1.33E-9 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.05|P-value:3.79E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.90|P-value:6.95E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.11|P-value:2.27E-4 ID:SGTC_2941|Compound:9056145|FD-Score:3.14|P-value:2.04E-4 ID:SGTC_2967|Compound:9089538|FD-Score:4.19|P-value:1.27E-6 ID:SGTC_2973|Compound:9094665|FD-Score:3.61|P-value:2.40E-5 ID:SGTC_3096|Compound:9117468|FD-Score:3.02|P-value:3.38E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-2.91|P-value:6.59E-4 ID:SGTC_3162|Compound:9099653|FD-Score:4.06|P-value:2.59E-6

Top fitness defect scores for YML100W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 5.30 3.7 µM 1.33E-9 24 5.64E-8
2 SGTC_2363 9053361 4.96 176.0 µM 1.32E-8 Golgi 15 3.56E-7
3 SGTC_1739 st037196 4.79 92.5 µM 3.68E-8 7 8.15E-7
4 SGTC_2294 7971431 4.20 200.0 µM 1.17E-6 11 1.32E-5
5 SGTC_2967 9089538 4.19 49.9 µM 1.27E-6 27 1.41E-5
6 SGTC_3162 9099653 4.06 49.5 µM 2.59E-6 5 2.50E-5
7 SGTC_2255 7941087 3.64 197.4 µM 2.17E-5 39 1.39E-4
8 SGTC_2973 9094665 3.61 18.0 µM 2.40E-5 NEO1 40 1.51E-4
9 SGTC_2103 5378138 3.53 10.0 µM 3.63E-5 28 2.10E-4
10 SGTC_916 4466-0038 3.49 42.0 nM 4.24E-5 cell wall 93 2.39E-4
11 SGTC_1620 st003704 3.28 16.5 µM 1.09E-4 37 5.12E-4
12 SGTC_6 0844-0013 3.19 19.8 µM 1.65E-4 177 7.15E-4
13 SGTC_2415 st077333 3.14 57.7 µM 2.04E-4 102 8.49E-4
14 SGTC_2941 9056145 3.14 11.2 µM 2.04E-4 36 8.50E-4
15 SGTC_2811 7967822 3.11 32.5 µM 2.27E-4 47 9.25E-4
16 SGTC_382 3229-0966 3.04 23.2 µM 3.05E-4 54 0.00118
17 SGTC_391 gliotoxin 3.02 8.3 µM 3.30E-4 101 0.00125
18 SGTC_3096 9117468 3.02 49.5 µM 3.38E-4 26 0.00128
19 SGTC_1779 st039427 2.79 55.0 µM 8.26E-4 107 0.00263
20 SGTC_1270 0831-1170 2.74 17.3 µM 9.90E-4 74 0.00305

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.232 3.20E-42 YPR023C EAF3 hom Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
0.211 3.77E-35 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.211 5.51E-35 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.177 5.35E-25 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.176 8.09E-25 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.171 1.82E-23 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.170 5.02E-23 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.161 7.45E-21 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.155 1.46E-19 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.155 2.04E-19 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.153 5.09E-19 YOR012W_p YOR012W_p hom Putative protein of unknown function
0.151 1.37E-18 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.150 2.23E-18 YER030W CHZ1 hom Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
0.150 2.66E-18 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.150 3.15E-18 YLR346C_p YLR346C_p hom Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene