YML105C / SEC65

Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML105C / SEC65

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:6.86|P-value:5.01E-13 ID:SGTC_37|Compound:0566-0070|FD-Score:3.38|P-value:2.23E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:7.36|P-value:1.02E-14 ID:SGTC_384|Compound:k007-0144|FD-Score:3.60|P-value:9.23E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_456|Compound:gingerol|FD-Score:-5.33|P-value:1.41E-8 ID:SGTC_459|Compound:nigericin|FD-Score:-4.34|P-value:3.09E-6 ID:SGTC_543|Compound:3844-1095|FD-Score:3.99|P-value:1.67E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:-4.83|P-value:2.43E-7 ID:SGTC_696|Compound:1560-0013|FD-Score:3.36|P-value:2.45E-4 ID:SGTC_698|Compound:k064-0052|FD-Score:3.21|P-value:4.22E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:3.84|P-value:3.28E-5 ID:SGTC_709|Compound:3807-1509|FD-Score:3.01|P-value:8.92E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.09|P-value:6.65E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.49|P-value:1.58E-6 ID:SGTC_960|Compound:1222-0186|FD-Score:6.11|P-value:1.05E-10 ID:SGTC_996|Compound:3970-0799|FD-Score:5.31|P-value:1.77E-8 ID:SGTC_1161|Compound:k007-0610|FD-Score:8.17|P-value:1.06E-17 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.01|P-value:8.61E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.01|P-value:8.51E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.29|P-value:3.23E-4 ID:SGTC_1621|Compound:st003705|FD-Score:-4.70|P-value:4.92E-7 ID:SGTC_1768|Compound:st044301|FD-Score:-3.28|P-value:3.21E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-3.22|P-value:3.92E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.75|P-value:4.96E-5 ID:SGTC_1956|Compound:st077085|FD-Score:-2.99|P-value:9.31E-4 ID:SGTC_2003|Compound:4023581|FD-Score:3.98|P-value:1.77E-5 ID:SGTC_2050|Compound:5263056|FD-Score:6.48|P-value:8.35E-12 ID:SGTC_2054|Compound:5233173|FD-Score:-3.36|P-value:2.36E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.05|P-value:7.61E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.01|P-value:8.87E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.10|P-value:6.54E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.29|P-value:3.12E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.26|P-value:3.47E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.00|P-value:8.82E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:-3.18|P-value:4.70E-4 ID:SGTC_2526|Compound:patulin|FD-Score:-3.40|P-value:1.95E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.94|P-value:2.14E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.80|P-value:3.71E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-3.82|P-value:3.48E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.02|P-value:8.51E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.07|P-value:7.19E-4 ID:SGTC_2938|Compound:9059746|FD-Score:3.62|P-value:8.33E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:6.86|P-value:5.01E-13 ID:SGTC_37|Compound:0566-0070|FD-Score:3.38|P-value:2.23E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:7.36|P-value:1.02E-14 ID:SGTC_384|Compound:k007-0144|FD-Score:3.60|P-value:9.23E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_456|Compound:gingerol|FD-Score:-5.33|P-value:1.41E-8 ID:SGTC_459|Compound:nigericin|FD-Score:-4.34|P-value:3.09E-6 ID:SGTC_543|Compound:3844-1095|FD-Score:3.99|P-value:1.67E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:-4.83|P-value:2.43E-7 ID:SGTC_696|Compound:1560-0013|FD-Score:3.36|P-value:2.45E-4 ID:SGTC_698|Compound:k064-0052|FD-Score:3.21|P-value:4.22E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:3.84|P-value:3.28E-5 ID:SGTC_709|Compound:3807-1509|FD-Score:3.01|P-value:8.92E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.09|P-value:6.65E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.49|P-value:1.58E-6 ID:SGTC_960|Compound:1222-0186|FD-Score:6.11|P-value:1.05E-10 ID:SGTC_996|Compound:3970-0799|FD-Score:5.31|P-value:1.77E-8 ID:SGTC_1161|Compound:k007-0610|FD-Score:8.17|P-value:1.06E-17 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.01|P-value:8.61E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.01|P-value:8.51E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.29|P-value:3.23E-4 ID:SGTC_1621|Compound:st003705|FD-Score:-4.70|P-value:4.92E-7 ID:SGTC_1768|Compound:st044301|FD-Score:-3.28|P-value:3.21E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-3.22|P-value:3.92E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.75|P-value:4.96E-5 ID:SGTC_1956|Compound:st077085|FD-Score:-2.99|P-value:9.31E-4 ID:SGTC_2003|Compound:4023581|FD-Score:3.98|P-value:1.77E-5 ID:SGTC_2050|Compound:5263056|FD-Score:6.48|P-value:8.35E-12 ID:SGTC_2054|Compound:5233173|FD-Score:-3.36|P-value:2.36E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.05|P-value:7.61E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.01|P-value:8.87E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.10|P-value:6.54E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.29|P-value:3.12E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.26|P-value:3.47E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.00|P-value:8.82E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:-3.18|P-value:4.70E-4 ID:SGTC_2526|Compound:patulin|FD-Score:-3.40|P-value:1.95E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.94|P-value:2.14E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.80|P-value:3.71E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-3.82|P-value:3.48E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.02|P-value:8.51E-4 ID:SGTC_2811|Compound:7967822|FD-Score:3.07|P-value:7.19E-4 ID:SGTC_2938|Compound:9059746|FD-Score:3.62|P-value:8.33E-5

Top fitness defect scores for YML105C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1161 k007-0610 8.17 110.0 µM 1.06E-17 ERAD & cell cycle 9 1.60E-16 0.07
2 SGTC_103 0844-0104 7.36 51.8 µM 1.02E-14 ERAD & cell cycle 16 9.27E-14 0.14
3 SGTC_16 erodoxin 6.86 22.8 µM 5.01E-13 unfolded protein response 7 3.43E-12 0.80
4 SGTC_2050 5263056 6.48 171.0 µM 8.35E-12 fatty acid desaturase (OLE1) 11 4.65E-11 0.52
5 SGTC_960 1222-0186 6.11 7.7 µM 1.05E-10 2 4.87E-10 0.95
6 SGTC_996 3970-0799 5.31 1.8 µM 1.77E-8 11 5.63E-8 0.15
7 SGTC_879 0831-1030 4.49 162.0 µM 1.58E-6 17 3.63E-6 0.07
8 SGTC_543 3844-1095 3.99 191.0 µM 1.67E-5 ERG2 6 3.24E-5 0.09
9 SGTC_2003 4023581 3.98 200.0 µM 1.77E-5 cell wall signaling 6 3.43E-5 0.03
10 SGTC_2645 abamectin 3.94 100.0 µM 2.14E-5 heme requiring 10 4.08E-5 0.02
11 SGTC_703 3875-0075 3.84 71.8 µM 3.28E-5 6 6.08E-5 0.20
12 SGTC_1814 st052110 3.75 31.5 µM 4.96E-5 RNA processing & uracil transport 10 8.92E-5 0.05
13 SGTC_2938 9059746 3.62 27.3 µM 8.33E-5 fatty acid desaturase (OLE1) 13 1.45E-4 0.02
14 SGTC_384 k007-0144 3.60 5.2 µM 9.23E-5 ERAD & cell cycle 17 1.59E-4 0.14
15 SGTC_37 0566-0070 3.38 1.6 µM 2.23E-4 7 3.61E-4 0.00
16 SGTC_696 1560-0013 3.36 611.0 µM 2.45E-4 16 3.94E-4 0.16
17 SGTC_2347 9049783 3.29 174.8 µM 3.12E-4 6 4.94E-4 0.04
18 SGTC_1606 st000094 3.29 49.2 µM 3.23E-4 60S ribosome export 13 5.09E-4 0.08
19 SGTC_698 k064-0052 3.21 127.0 µM 4.22E-4 DNA damage response 7 6.53E-4 0.03
20 SGTC_2323 6148992 3.10 148.3 µM 6.54E-4 21 9.83E-4 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.334 2.61E-88 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.254 3.39E-50 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.247 8.48E-48 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.246 2.80E-47 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.232 2.66E-42 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.222 1.12E-38 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.217 6.49E-37 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.216 1.52E-36 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.212 1.67E-35 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.208 4.55E-34 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.204 6.77E-33 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.198 8.20E-31 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.186 2.03E-27 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.184 9.53E-27 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.183 1.24E-26 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress