YML112W / CTK3

Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YML112W / CTK3

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:4.03|P-value:8.98E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.10|P-value:7.40E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.22|P-value:2.54E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:4.86|P-value:8.14E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:5.57|P-value:7.79E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.90|P-value:6.14E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.07|P-value:7.09E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:4.58|P-value:1.91E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.88|P-value:6.63E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:-4.01|P-value:8.45E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.93|P-value:5.75E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:4.17|P-value:6.14E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:4.45|P-value:2.82E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:4.73|P-value:1.22E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.26|P-value:4.85E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:4.20|P-value:5.73E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:-4.26|P-value:4.27E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-6.00|P-value:1.33E-6 ID:SGTC_1167|Compound:k781-0610|FD-Score:5.90|P-value:2.32E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:4.22|P-value:5.37E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:5.10|P-value:3.82E-5 ID:SGTC_1385|Compound:3091-4834|FD-Score:5.03|P-value:4.83E-5 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.07|P-value:8.13E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:4.54|P-value:2.14E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.28|P-value:4.59E-4 ID:SGTC_1622|Compound:st003707|FD-Score:4.66|P-value:1.52E-4 ID:SGTC_1656|Compound:st012921|FD-Score:7.84|P-value:5.59E-10 ID:SGTC_1658|Compound:st012944|FD-Score:-4.22|P-value:4.76E-4 ID:SGTC_1715|Compound:st032160|FD-Score:-7.16|P-value:1.10E-8 ID:SGTC_1745|Compound:st081495|FD-Score:4.62|P-value:1.72E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.47|P-value:1.08E-5 ID:SGTC_1781|Compound:st038725|FD-Score:4.37|P-value:3.55E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-4.25|P-value:4.33E-4 ID:SGTC_1944|Compound:st076371|FD-Score:4.02|P-value:9.22E-4 ID:SGTC_2090|Compound:5301565|FD-Score:4.21|P-value:5.45E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-4.45|P-value:2.43E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.60|P-value:1.83E-4 ID:SGTC_2192|Compound:5915882|FD-Score:6.20|P-value:7.32E-7 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.52|P-value:1.97E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:5.27|P-value:2.18E-5 ID:SGTC_2416|Compound:tofa|FD-Score:-6.26|P-value:4.83E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.81|P-value:8.32E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-4.03|P-value:7.86E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:5.95|P-value:1.97E-6 ID:SGTC_2793|Compound:7325131|FD-Score:-4.81|P-value:8.22E-5 ID:SGTC_2797|Compound:7859098|FD-Score:4.76|P-value:1.11E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-4.05|P-value:7.56E-4 ID:SGTC_3087|Compound:9117406|FD-Score:-6.71|P-value:7.62E-8 ID:SGTC_3201|Compound:9113999|FD-Score:4.10|P-value:7.39E-4 ID:SGTC_3291|Compound:9121177|FD-Score:4.23|P-value:5.27E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-4.27|P-value:4.07E-4 ID:SGTC_3303|Compound:9125618|FD-Score:6.37|P-value:3.76E-7 ID:SGTC_3305|Compound:9127027|FD-Score:4.21|P-value:5.58E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-5.66|P-value:4.73E-6 ID:SGTC_20|Compound:0481-0013|FD-Score:4.03|P-value:8.98E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.10|P-value:7.40E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.22|P-value:2.54E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:4.86|P-value:8.14E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:5.57|P-value:7.79E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.90|P-value:6.14E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.07|P-value:7.09E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:4.58|P-value:1.91E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.88|P-value:6.63E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:-4.01|P-value:8.45E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.93|P-value:5.75E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:4.17|P-value:6.14E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:4.45|P-value:2.82E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:4.73|P-value:1.22E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.26|P-value:4.85E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:4.20|P-value:5.73E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:-4.26|P-value:4.27E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-6.00|P-value:1.33E-6 ID:SGTC_1167|Compound:k781-0610|FD-Score:5.90|P-value:2.32E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:4.22|P-value:5.37E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:5.10|P-value:3.82E-5 ID:SGTC_1385|Compound:3091-4834|FD-Score:5.03|P-value:4.83E-5 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.07|P-value:8.13E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:4.54|P-value:2.14E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.28|P-value:4.59E-4 ID:SGTC_1622|Compound:st003707|FD-Score:4.66|P-value:1.52E-4 ID:SGTC_1656|Compound:st012921|FD-Score:7.84|P-value:5.59E-10 ID:SGTC_1658|Compound:st012944|FD-Score:-4.22|P-value:4.76E-4 ID:SGTC_1715|Compound:st032160|FD-Score:-7.16|P-value:1.10E-8 ID:SGTC_1745|Compound:st081495|FD-Score:4.62|P-value:1.72E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.47|P-value:1.08E-5 ID:SGTC_1781|Compound:st038725|FD-Score:4.37|P-value:3.55E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-4.25|P-value:4.33E-4 ID:SGTC_1944|Compound:st076371|FD-Score:4.02|P-value:9.22E-4 ID:SGTC_2090|Compound:5301565|FD-Score:4.21|P-value:5.45E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-4.45|P-value:2.43E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.60|P-value:1.83E-4 ID:SGTC_2192|Compound:5915882|FD-Score:6.20|P-value:7.32E-7 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.52|P-value:1.97E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:5.27|P-value:2.18E-5 ID:SGTC_2416|Compound:tofa|FD-Score:-6.26|P-value:4.83E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.81|P-value:8.32E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-4.03|P-value:7.86E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:5.95|P-value:1.97E-6 ID:SGTC_2793|Compound:7325131|FD-Score:-4.81|P-value:8.22E-5 ID:SGTC_2797|Compound:7859098|FD-Score:4.76|P-value:1.11E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-4.05|P-value:7.56E-4 ID:SGTC_3087|Compound:9117406|FD-Score:-6.71|P-value:7.62E-8 ID:SGTC_3201|Compound:9113999|FD-Score:4.10|P-value:7.39E-4 ID:SGTC_3291|Compound:9121177|FD-Score:4.23|P-value:5.27E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-4.27|P-value:4.07E-4 ID:SGTC_3303|Compound:9125618|FD-Score:6.37|P-value:3.76E-7 ID:SGTC_3305|Compound:9127027|FD-Score:4.21|P-value:5.58E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-5.66|P-value:4.73E-6

Top fitness defect scores for YML112W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1656 st012921 7.84 11.0 µM 5.59E-10 12 2.25E-15
2 SGTC_3303 9125618 6.37 40.6 µM 3.76E-7 10 9.34E-11
3 SGTC_2192 5915882 6.20 198.5 µM 7.32E-7 RNA processing & uracil transport 10 2.76E-10
4 SGTC_2533 agelasine 5.95 13.7 µM 1.97E-6 ERG2 11 1.38E-9
5 SGTC_1167 k781-0610 5.90 148.0 µM 2.32E-6 RNA processing & uracil transport 3 1.80E-9
6 SGTC_187 1488-2104 5.57 32.7 µM 7.79E-6 calcium & mitochondrial duress 19 1.29E-8
7 SGTC_1750 st081859 5.47 2.4 µM 1.08E-5 endomembrane recycling 123 2.19E-8
8 SGTC_2409 alverine citrate 5.27 64.0 µM 2.18E-5 29 6.87E-8
9 SGTC_146 3448-8650 5.22 53.1 µM 2.54E-5 NEO1-PIK1 73 8.79E-8
10 SGTC_1226 3909-7979 5.10 35.4 µM 3.82E-5 21 1.71E-7
11 SGTC_1385 3091-4834 5.03 14.1 µM 4.83E-5 RPP1 & pyrimidine depletion 3 2.50E-7
12 SGTC_178 4092-0806 4.86 87.5 µM 8.14E-5 ergosterol depletion effects on membrane 59 5.81E-7
13 SGTC_2797 7859098 4.76 81.8 µM 1.11E-4 12 9.62E-7
14 SGTC_710 3448-4187 4.73 58.0 µM 1.22E-4 fatty acid desaturase (OLE1) 32 1.12E-6
15 SGTC_1622 st003707 4.66 19.4 µM 1.52E-4 calcium & mitochondrial duress 22 1.60E-6
16 SGTC_1745 st081495 4.62 74.0 µM 1.72E-4 RNA processing & uracil transport 54 1.94E-6
17 SGTC_2137 5261020 4.60 140.0 µM 1.83E-4 27 2.14E-6
18 SGTC_366 0181-0206 4.58 4.3 µM 1.91E-4 Golgi 23 2.31E-6
19 SGTC_1581 himbacine 4.54 57.9 µM 2.14E-4 Golgi 40 2.77E-6
20 SGTC_646 4092-0839 4.45 27.3 µM 2.82E-4 plasma membrane duress 15 4.31E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.160 1.06E-20 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.142 1.73E-16 YEL031W SPF1 hom P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
0.140 4.12E-16 YIL154C IMP2' hom Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
0.139 5.68E-16 YGR155W CYS4 hom Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
0.139 8.05E-16 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.137 1.47E-15 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.136 2.22E-15 YDR173C ARG82 hom Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
0.126 2.42E-13 YDR159W SAC3 hom Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
0.122 1.10E-12 YGL244W RTF1 hom Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
0.121 2.86E-12 YNL323W LEM3 hom Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
0.117 1.04E-11 YBL007C SLA1 hom Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
0.116 1.69E-11 YOL072W THP1 hom Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
0.115 2.26E-11 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.115 2.31E-11 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.115 3.05E-11 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication