HIPHOP chemogenomics database

YML115C / VAN1

Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant

Zygosity: Homozygous strain

fixedexpanded
Profile for YML115C / VAN1

Click on Significant Values for Screen Details

Top fitness defect scores for YML115C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2289	7946404	8.79	165.1 µM	9.97E-15		10	7.78E-19
2	SGTC_1873	rapamycin	6.60	2.0 nM	5.19E-9		25	2.06E-11
3	SGTC_2582	agaric acid	6.44	100.0 µM	1.17E-8	redox potentiating	143	5.93E-11
4	SGTC_2689	tacrolimus	6.39	100.0 µM	1.54E-8		9	8.46E-11
5	SGTC_658	3253-0951	6.24	102.0 µM	3.28E-8	calcium & mitochondrial duress	12	2.26E-10
6	SGTC_1473	k844-0725	5.69	134.0 µM	4.22E-7		2	6.25E-9
7	SGTC_1991	st073000	5.41	8.9 µM	1.45E-6	NEO1	45	3.10E-8
8	SGTC_2695	st077686	5.41	52.0 µM	1.47E-6		13	3.17E-8
9	SGTC_2983	9022671	5.37	13.0 µM	1.70E-6	60S ribosome export	3	3.83E-8
10	SGTC_3152	9099253	5.35	49.5 µM	1.87E-6	NEO1-PIK1	71	4.33E-8
11	SGTC_2737	bleomycin	5.31	30.5 nM	2.24E-6	Golgi	39	5.47E-8
12	SGTC_2283	7944793	5.19	200.0 µM	3.67E-6		15	1.04E-7
13	SGTC_708	3448-5400	5.16	146.0 µM	4.20E-6		15	1.24E-7
14	SGTC_2697	mebhydrolin	4.94	72.4 µM	1.02E-5	NEO1	46	3.91E-7
15	SGTC_2821	7996764	4.89	71.4 µM	1.26E-5		17	5.16E-7
16	SGTC_1627	st003713	4.77	25.6 µM	1.97E-5	calcium & mitochondrial duress	45	9.24E-7
17	SGTC_146	3448-8650	4.77	53.1 µM	1.98E-5	NEO1-PIK1	95	9.29E-7
18	SGTC_1623	st003709	4.71	20.3 µM	2.50E-5	calcium & mitochondrial duress	38	1.26E-6
19	SGTC_2199	6590217	4.69	200.0 µM	2.69E-5	mitochondrial processes	2	1.39E-6
20	SGTC_2345	9052315	4.67	157.4 µM	2.86E-5		10	1.50E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.206	1.61E-33	YOR002W	ALG6	hom	Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.198	4.67E-31	YLR342W	FKS1	hom	Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.181	5.42E-26	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.172	1.33E-23	YGR166W	TRS65	hom	Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.168	1.38E-22	YDL117W	CYK3	hom	SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.167	1.83E-22	YDR525W_d	API2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
0.167	1.89E-22	YBL082C	ALG3	hom	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.165	6.16E-22	YGL027C	CWH41	hom	Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
0.165	6.68E-22	YDR372C	VPS74	hom	Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.163	1.82E-21	YML019W	OST6	hom	Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
0.159	2.07E-20	YJR075W	HOC1	hom	Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.151	1.75E-18	YBR229C	ROT2	hom	Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.150	2.87E-18	YOR039W	CKB2	hom	Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.149	4.36E-18	YAL058W	CNE1	hom	Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.148	6.19E-18	YEL036C	ANP1	hom	Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol