YML123C / PHO84

High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity

Zygosity: Homozygous strain
fixedexpanded
Profile for YML123C / PHO84

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:5.17|P-value:9.01E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.58|P-value:7.30E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.97|P-value:5.03E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.67|P-value:1.31E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:-3.81|P-value:7.96E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.12|P-value:1.08E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.80|P-value:8.04E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-4.79|P-value:3.53E-5 ID:SGTC_161|Compound:4065-0411|FD-Score:15.70|P-value:3.73E-39 ID:SGTC_173|Compound:1273-0061|FD-Score:4.21|P-value:2.42E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:4.70|P-value:4.80E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:3.94|P-value:5.33E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.84|P-value:2.97E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.75|P-value:9.45E-7 ID:SGTC_356|Compound:1634-0012|FD-Score:4.14|P-value:2.99E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-4.38|P-value:1.41E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-9.50|P-value:1.60E-15 ID:SGTC_375|Compound:3970-0961|FD-Score:5.26|P-value:6.29E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.35|P-value:1.54E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-5.18|P-value:8.54E-6 ID:SGTC_470|Compound:aa-861|FD-Score:6.46|P-value:4.13E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:4.30|P-value:1.79E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:7.94|P-value:2.24E-11 ID:SGTC_639|Compound:0833-0042|FD-Score:4.33|P-value:1.66E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.94|P-value:5.48E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-4.46|P-value:1.10E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.73|P-value:4.36E-5 ID:SGTC_718|Compound:1130-0073|FD-Score:3.84|P-value:7.14E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:5.82|P-value:6.93E-7 ID:SGTC_741|Compound:4582-0002|FD-Score:3.95|P-value:5.18E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:6.85|P-value:6.68E-9 ID:SGTC_764|Compound:1951-2361|FD-Score:4.71|P-value:4.73E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.80|P-value:8.03E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.28|P-value:1.95E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.91|P-value:5.85E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:4.55|P-value:7.99E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:5.32|P-value:5.18E-6 ID:SGTC_942|Compound:3253-1115|FD-Score:4.30|P-value:1.81E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:3.92|P-value:5.78E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:7.03|P-value:2.71E-9 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.20|P-value:2.49E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.74|P-value:4.15E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:4.63|P-value:6.21E-5 ID:SGTC_1407|Compound:3909-7909|FD-Score:-4.17|P-value:2.70E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.82|P-value:7.65E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-4.91|P-value:2.30E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-5.11|P-value:1.11E-5 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:4.23|P-value:2.28E-4 ID:SGTC_1658|Compound:st012944|FD-Score:-4.00|P-value:4.60E-4 ID:SGTC_1673|Compound:st016611|FD-Score:4.46|P-value:1.08E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-4.20|P-value:2.51E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.20|P-value:2.50E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.88|P-value:6.44E-4 ID:SGTC_1831|Compound:st055328|FD-Score:4.01|P-value:4.44E-4 ID:SGTC_1851|Compound:st056236|FD-Score:4.17|P-value:2.70E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.83|P-value:7.52E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.84|P-value:2.96E-5 ID:SGTC_2090|Compound:5301565|FD-Score:6.82|P-value:7.85E-9 ID:SGTC_2137|Compound:5261020|FD-Score:4.11|P-value:3.25E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:7.52|P-value:2.18E-10 ID:SGTC_2296|Compound:7971656|FD-Score:5.82|P-value:7.04E-7 ID:SGTC_2326|Compound:6164455|FD-Score:-3.79|P-value:8.39E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-4.28|P-value:1.95E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-5.03|P-value:1.53E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.89|P-value:6.26E-4 ID:SGTC_2523|Compound:spermine|FD-Score:7.36|P-value:5.11E-10 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:4.24|P-value:2.17E-4 ID:SGTC_2701|Compound:st077767|FD-Score:4.27|P-value:1.96E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-3.82|P-value:7.61E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-5.58|P-value:1.84E-6 ID:SGTC_2845|Compound:9003024|FD-Score:4.08|P-value:3.51E-4 ID:SGTC_2871|Compound:9038510|FD-Score:-4.07|P-value:3.67E-4 ID:SGTC_2921|Compound:7974240|FD-Score:6.45|P-value:4.34E-8 ID:SGTC_3002|Compound:9075603|FD-Score:5.38|P-value:4.05E-6 ID:SGTC_3054|Compound:9094397|FD-Score:5.17|P-value:9.06E-6 ID:SGTC_3327|Compound:9140648|FD-Score:5.19|P-value:8.20E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:5.17|P-value:9.01E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.58|P-value:7.30E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.97|P-value:5.03E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.67|P-value:1.31E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:-3.81|P-value:7.96E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.12|P-value:1.08E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.80|P-value:8.04E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-4.79|P-value:3.53E-5 ID:SGTC_161|Compound:4065-0411|FD-Score:15.70|P-value:3.73E-39 ID:SGTC_173|Compound:1273-0061|FD-Score:4.21|P-value:2.42E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:4.70|P-value:4.80E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:3.94|P-value:5.33E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.84|P-value:2.97E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.75|P-value:9.45E-7 ID:SGTC_356|Compound:1634-0012|FD-Score:4.14|P-value:2.99E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-4.38|P-value:1.41E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-9.50|P-value:1.60E-15 ID:SGTC_375|Compound:3970-0961|FD-Score:5.26|P-value:6.29E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.35|P-value:1.54E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-5.18|P-value:8.54E-6 ID:SGTC_470|Compound:aa-861|FD-Score:6.46|P-value:4.13E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:4.30|P-value:1.79E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:7.94|P-value:2.24E-11 ID:SGTC_639|Compound:0833-0042|FD-Score:4.33|P-value:1.66E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.94|P-value:5.48E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-4.46|P-value:1.10E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.73|P-value:4.36E-5 ID:SGTC_718|Compound:1130-0073|FD-Score:3.84|P-value:7.14E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:5.82|P-value:6.93E-7 ID:SGTC_741|Compound:4582-0002|FD-Score:3.95|P-value:5.18E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:6.85|P-value:6.68E-9 ID:SGTC_764|Compound:1951-2361|FD-Score:4.71|P-value:4.73E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.80|P-value:8.03E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.28|P-value:1.95E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.91|P-value:5.85E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:4.55|P-value:7.99E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:5.32|P-value:5.18E-6 ID:SGTC_942|Compound:3253-1115|FD-Score:4.30|P-value:1.81E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:3.92|P-value:5.78E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:7.03|P-value:2.71E-9 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.20|P-value:2.49E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.74|P-value:4.15E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:4.63|P-value:6.21E-5 ID:SGTC_1407|Compound:3909-7909|FD-Score:-4.17|P-value:2.70E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.82|P-value:7.65E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-4.91|P-value:2.30E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-5.11|P-value:1.11E-5 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:4.23|P-value:2.28E-4 ID:SGTC_1658|Compound:st012944|FD-Score:-4.00|P-value:4.60E-4 ID:SGTC_1673|Compound:st016611|FD-Score:4.46|P-value:1.08E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-4.20|P-value:2.51E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.20|P-value:2.50E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.88|P-value:6.44E-4 ID:SGTC_1831|Compound:st055328|FD-Score:4.01|P-value:4.44E-4 ID:SGTC_1851|Compound:st056236|FD-Score:4.17|P-value:2.70E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.83|P-value:7.52E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.84|P-value:2.96E-5 ID:SGTC_2090|Compound:5301565|FD-Score:6.82|P-value:7.85E-9 ID:SGTC_2137|Compound:5261020|FD-Score:4.11|P-value:3.25E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:7.52|P-value:2.18E-10 ID:SGTC_2296|Compound:7971656|FD-Score:5.82|P-value:7.04E-7 ID:SGTC_2326|Compound:6164455|FD-Score:-3.79|P-value:8.39E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-4.28|P-value:1.95E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-5.03|P-value:1.53E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.89|P-value:6.26E-4 ID:SGTC_2523|Compound:spermine|FD-Score:7.36|P-value:5.11E-10 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:4.24|P-value:2.17E-4 ID:SGTC_2701|Compound:st077767|FD-Score:4.27|P-value:1.96E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-3.82|P-value:7.61E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-5.58|P-value:1.84E-6 ID:SGTC_2845|Compound:9003024|FD-Score:4.08|P-value:3.51E-4 ID:SGTC_2871|Compound:9038510|FD-Score:-4.07|P-value:3.67E-4 ID:SGTC_2921|Compound:7974240|FD-Score:6.45|P-value:4.34E-8 ID:SGTC_3002|Compound:9075603|FD-Score:5.38|P-value:4.05E-6 ID:SGTC_3054|Compound:9094397|FD-Score:5.17|P-value:9.06E-6 ID:SGTC_3327|Compound:9140648|FD-Score:5.19|P-value:8.20E-6

Top fitness defect scores for YML123C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_161 4065-0411 15.70 162.2 µM 3.73E-39 2 5.71E-56
2 SGTC_581 0958-0271 7.94 211.0 µM 2.24E-11 10 1.00E-15
3 SGTC_2232 chlorophetanol 7.52 41.5 µM 2.18E-10 TRP & mitochondrial translation 23 2.67E-14
4 SGTC_2523 spermine 7.36 47.7 µM 5.11E-10 calcium & mitochondrial duress 7 9.14E-14
5 SGTC_974 1326-0144 7.03 138.0 µM 2.71E-9 ERG2 6 1.01E-12
6 SGTC_763 3453-1109 6.85 128.0 µM 6.68E-9 2 3.73E-12
7 SGTC_2090 5301565 6.82 104.5 µM 7.85E-9 TSC3-RPN4 15 4.70E-12
8 SGTC_470 aa-861 6.46 153.0 µM 4.13E-8 sphingolipid biosynthesis & PDR1 20 5.14E-11
9 SGTC_2921 7974240 6.45 10.7 µM 4.34E-8 12 5.50E-11
10 SGTC_738 1315-0003 5.82 72.4 µM 6.93E-7 3 2.96E-9
11 SGTC_2296 7971656 5.82 126.5 µM 7.04E-7 15 3.03E-9
12 SGTC_3002 9075603 5.38 71.4 µM 4.05E-6 3 3.73E-8
13 SGTC_879 0831-1030 5.32 162.0 µM 5.18E-6 17 5.33E-8
14 SGTC_375 3970-0961 5.26 9.3 µM 6.29E-6 tubulin folding & SWR complex 7 7.03E-8
15 SGTC_3327 9140648 5.19 26.9 µM 8.20E-6 4 1.03E-7
16 SGTC_56 0957-0365 5.17 83.2 µM 9.01E-6 Golgi 23 1.18E-7
17 SGTC_3054 9094397 5.17 49.5 µM 9.06E-6 fatty acid desaturase (OLE1) 11 1.19E-7
18 SGTC_146 3448-8650 5.12 53.1 µM 1.08E-5 NEO1-PIK1 82 1.52E-7
19 SGTC_1877 wortmannin 4.84 25.0 µM 2.96E-5 68 6.45E-7
20 SGTC_1173 0443-0020 4.74 122.0 µM 4.15E-5 TSC3-RPN4 20 1.05E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.224 3.10E-39 YIR023W DAL81 hom Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
0.212 2.91E-35 YFR034C PHO4 hom Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
0.188 5.08E-28 YML122C_d YML122C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.150 2.40E-18 YHL011C PRS3 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
0.143 8.74E-17 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.138 9.06E-16 YDR463W STP1 hom Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
0.134 5.76E-15 YMR183C SSO2 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
0.131 3.88E-14 YHR062C RPP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.129 6.00E-14 YJR063W RPA12 hom RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.129 8.61E-14 YDR127W ARO1 hom Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.127 1.56E-13 YER107C GLE2 hom RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
0.123 8.05E-13 YML124C TUB3 hom Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.121 1.83E-12 YLR451W LEU3 hom Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
0.117 1.27E-11 YBR069C TAT1 hom Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
0.116 1.52E-11 YGR063C SPT4 hom Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions