HIPHOP chemogenomics database

YML124C / TUB3

Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YML124C / TUB3

Click on Significant Values for Screen Details

Top fitness defect scores for YML124C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_229	benomyl	20.80	22.9 µM	4.66E-55	tubulin folding & SWR complex	2	3.24E-96
2	SGTC_2319	6033012	18.10	152.6 µM	4.71E-42	tubulin folding & SWR complex	4	3.48E-73
3	SGTC_210	3551-0445	17.60	56.8 µM	4.02E-40		5	9.13E-70
4	SGTC_1953	st076800	16.30	12.1 µM	1.72E-34		1	8.55E-60
5	SGTC_638	3970-0837	16.20	4.1 µM	3.27E-34		1	2.66E-59
6	SGTC_589	1227-0070	15.00	34.1 µM	1.03E-29		3	2.44E-51
7	SGTC_1352	1496-0991	14.80	99.0 µM	4.75E-29	tubulin folding & SWR complex	1	3.65E-50
8	SGTC_2200	6600604	14.50	21.9 µM	6.60E-28	tubulin folding & SWR complex	6	3.83E-48
9	SGTC_149	3970-0822	13.70	46.1 µM	4.66E-25	tubulin folding & SWR complex	6	4.21E-43
10	SGTC_2510	benzyl isothiocyanate	13.40	8.3 µM	4.49E-24		1	2.32E-41
11	SGTC_286	k060-0067	12.30	22.9 µM	1.78E-20	tubulin folding & SWR complex	2	5.36E-35
12	SGTC_2125	5246583	12.10	200.0 µM	4.84E-20	tubulin folding & SWR complex	2	3.14E-34
13	SGTC_897	1000-0139	11.80	31.4 µM	4.45E-19		3	1.59E-32
14	SGTC_2254	7938975	11.80	95.2 µM	6.69E-19		9	3.25E-32
15	SGTC_3282	9108498	11.10	15.5 µM	5.72E-17		3	8.46E-29
16	SGTC_2845	9003024	11.10	43.0 µM	6.46E-17	amide catabolism	3	1.05E-28
17	SGTC_3294	9122953	11.00	26.3 µM	8.90E-17	tubulin folding & SWR complex	1	1.85E-28
18	SGTC_2952	9073731	10.90	13.8 µM	1.82E-16	tubulin folding & SWR complex	2	6.50E-28
19	SGTC_2534	helenine	10.60	20.1 µM	1.09E-15	RSC complex & mRNA processing	1	1.54E-26
20	SGTC_1875	nocodazole	10.60	6.0 µM	1.39E-15	tubulin folding & SWR complex	13	2.38E-26

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.648	0	YER007W	PAC2	hom	Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.608	0	YOR349W	CIN1	hom	Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.529	3.68E-241	YPL241C	CIN2	hom	GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.524	3.22E-236	YLR085C	ARP6	hom	Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.494	4.56E-205	YML041C	VPS71	hom	Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.485	1.44E-197	YDR485C	VPS72	hom	Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.481	1.85E-193	YAL011W	SWC3	hom	Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
0.451	6.02E-168	YDR334W	SWR1	hom	Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.367	2.77E-107	YBR231C	SWC5	hom	Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
0.360	2.49E-103	YOL012C	HTZ1	hom	Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
0.312	2.48E-76	YAL024C	LTE1	hom	Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.276	1.40E-59	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.271	3.35E-57	YNL298W	CLA4	hom	Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
0.244	1.07E-46	YNL148C	ALF1	hom	Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
0.240	3.52E-45	YLR243W	GPN3	het	Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p