YMR005W / TAF4

TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR005W / TAF4

Click on Significant Values for Screen Details ID:SGTC_490|Compound:phenamil|FD-Score:3.47|P-value:4.71E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:-3.22|P-value:9.38E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:-3.68|P-value:1.92E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:3.57|P-value:3.27E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:6.89|P-value:2.22E-11 ID:SGTC_743|Compound:1275-0778|FD-Score:4.62|P-value:5.21E-6 ID:SGTC_746|Compound:1095-0025|FD-Score:-3.36|P-value:5.85E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.26|P-value:9.32E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.78|P-value:1.31E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:4.16|P-value:3.59E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.73|P-value:1.57E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:4.39|P-value:1.36E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.56|P-value:3.40E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.73|P-value:3.19E-6 ID:SGTC_2012|Compound:4023501|FD-Score:3.33|P-value:7.52E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-4.22|P-value:2.34E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.33|P-value:6.36E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.46|P-value:4.11E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-4.03|P-value:5.03E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:-4.00|P-value:5.64E-5 ID:SGTC_2840|Compound:9008771|FD-Score:-4.32|P-value:1.50E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-3.26|P-value:8.02E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.47|P-value:4.71E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:-3.22|P-value:9.38E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:-3.68|P-value:1.92E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:3.57|P-value:3.27E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:6.89|P-value:2.22E-11 ID:SGTC_743|Compound:1275-0778|FD-Score:4.62|P-value:5.21E-6 ID:SGTC_746|Compound:1095-0025|FD-Score:-3.36|P-value:5.85E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.26|P-value:9.32E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.78|P-value:1.31E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:4.16|P-value:3.59E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.73|P-value:1.57E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:4.39|P-value:1.36E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.56|P-value:3.40E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.73|P-value:3.19E-6 ID:SGTC_2012|Compound:4023501|FD-Score:3.33|P-value:7.52E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-4.22|P-value:2.34E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.33|P-value:6.36E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-3.46|P-value:4.11E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-4.03|P-value:5.03E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:-4.00|P-value:5.64E-5 ID:SGTC_2840|Compound:9008771|FD-Score:-4.32|P-value:1.50E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-3.26|P-value:8.02E-4

Top fitness defect scores for YMR005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_741 4582-0002 6.89 126.0 µM 2.22E-11 1 2.84E-12 1.70
2 SGTC_1844 rapamycin 4.73 4.0 nM 3.19E-6 37 1.15E-6 0.18
3 SGTC_743 1275-0778 4.62 158.0 µM 5.21E-6 excess fatty acid 9 1.96E-6 0.04
4 SGTC_1322 1348-1070 4.39 12.0 µM 1.36E-5 1 5.58E-6 0.60
5 SGTC_1080 nisoldipine 4.16 24.4 µM 3.59E-5 TSC3-RPN4 4 1.60E-5 0.13
6 SGTC_713 1488-1073 3.57 324.0 µM 3.27E-4 ERG2 3 1.76E-4 0.08
7 SGTC_1328 1441-0025 3.56 69.2 µM 3.40E-4 5 1.84E-4 0.33
8 SGTC_490 phenamil 3.47 164.0 µM 4.71E-4 heme requiring 6 2.61E-4 0.20
9 SGTC_2012 4023501 3.33 200.0 µM 7.52E-4 18 4.35E-4 0.01
10 SGTC_883 0850-0245 3.26 24.6 µM 9.32E-4 cell wall 12 5.49E-4 0.02
11 SGTC_2023 5144124 3.19 124.0 µM 0.00119 14 7.18E-4 0.15
12 SGTC_1365 1672-3266 3.15 55.4 µM 0.00136 16 8.25E-4 0.04
13 SGTC_1324 1418-0026 3.12 34.4 µM 0.00150 7 9.20E-4 0.00
14 SGTC_1077 5-fluorocytosine 3.08 377.0 nM 0.00167 exosome 26 0.00103 0.08
15 SGTC_1352 1496-0991 3.05 99.0 µM 0.00182 tubulin folding & SWR complex 4 0.00114 0.12
16 SGTC_1744 st037937 3.04 38.9 µM 0.00187 14 0.00117 0.04
17 SGTC_714 4296-0578 3.04 26.1 µM 0.00191 2 0.00120 0.05
18 SGTC_1747 st037496 3.03 66.6 µM 0.00193 22 0.00121 0.02
19 SGTC_2922 7999257 2.99 32.5 µM 0.00221 17 0.00140 0.06
20 SGTC_3272 9137413 2.98 49.5 µM 0.00224 22 0.00142 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 3.70E-17 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.135 3.99E-15 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.128 1.05E-13 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.128 1.06E-13 YDR479C PEX29 hom Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
0.122 1.52E-12 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.121 2.15E-12 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.116 1.51E-11 YDR490C PKH1 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p
0.110 1.51E-10 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.105 9.83E-10 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.103 1.90E-9 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.101 3.96E-9 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.097 1.70E-8 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.094 4.34E-8 YKR007W MEH1 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
0.093 6.96E-8 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.092 9.33E-8 YFL001W DEG1 hom tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability