YMR013C / SEC59

Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR013C / SEC59

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:-3.04|P-value:4.68E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:2.90|P-value:7.95E-4 ID:SGTC_202|Compound:3594-0513|FD-Score:2.96|P-value:6.50E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.34|P-value:1.41E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.86|P-value:9.49E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.22|P-value:2.27E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:-2.87|P-value:8.80E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.15|P-value:3.06E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.87|P-value:1.26E-5 ID:SGTC_428|Compound:3448-4153|FD-Score:3.51|P-value:6.67E-5 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.90|P-value:4.87E-8 ID:SGTC_522|Compound:1273-0010|FD-Score:3.27|P-value:1.86E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.22|P-value:2.27E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.55|P-value:5.63E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:3.59|P-value:4.75E-5 ID:SGTC_919|Compound:tnp00227|FD-Score:3.18|P-value:2.74E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:2.99|P-value:5.72E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:2.95|P-value:6.68E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:2.85|P-value:9.52E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.44|P-value:8.97E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.35|P-value:1.11E-6 ID:SGTC_1488|Compound:4013-0813|FD-Score:2.89|P-value:8.42E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-3.30|P-value:1.66E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-3.21|P-value:2.38E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.65|P-value:3.52E-5 ID:SGTC_1749|Compound:st037932|FD-Score:2.90|P-value:8.19E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.60|P-value:4.44E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.45|P-value:8.63E-5 ID:SGTC_2000|Compound:st075254|FD-Score:3.09|P-value:3.93E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.39|P-value:8.75E-7 ID:SGTC_2110|Compound:5483698|FD-Score:-2.92|P-value:7.31E-4 ID:SGTC_2315|Compound:7733312|FD-Score:2.94|P-value:6.95E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.52|P-value:6.42E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.43|P-value:7.00E-7 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.25|P-value:2.07E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:2.85|P-value:9.66E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:7.11|P-value:5.04E-15 ID:SGTC_2692|Compound:st077426|FD-Score:3.05|P-value:4.58E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.93|P-value:9.53E-6 ID:SGTC_2806|Compound:7952793|FD-Score:-2.90|P-value:7.85E-4 ID:SGTC_3012|Compound:9080433|FD-Score:2.90|P-value:8.02E-4 ID:SGTC_3179|Compound:9107801|FD-Score:2.99|P-value:5.63E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.41|P-value:1.04E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.04|P-value:4.68E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:2.90|P-value:7.95E-4 ID:SGTC_202|Compound:3594-0513|FD-Score:2.96|P-value:6.50E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.34|P-value:1.41E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.86|P-value:9.49E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.22|P-value:2.27E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:-2.87|P-value:8.80E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.15|P-value:3.06E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.87|P-value:1.26E-5 ID:SGTC_428|Compound:3448-4153|FD-Score:3.51|P-value:6.67E-5 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.90|P-value:4.87E-8 ID:SGTC_522|Compound:1273-0010|FD-Score:3.27|P-value:1.86E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.22|P-value:2.27E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.55|P-value:5.63E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:3.59|P-value:4.75E-5 ID:SGTC_919|Compound:tnp00227|FD-Score:3.18|P-value:2.74E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:2.99|P-value:5.72E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:2.95|P-value:6.68E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:2.85|P-value:9.52E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.44|P-value:8.97E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.35|P-value:1.11E-6 ID:SGTC_1488|Compound:4013-0813|FD-Score:2.89|P-value:8.42E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-3.30|P-value:1.66E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-3.21|P-value:2.38E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.65|P-value:3.52E-5 ID:SGTC_1749|Compound:st037932|FD-Score:2.90|P-value:8.19E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.60|P-value:4.44E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.45|P-value:8.63E-5 ID:SGTC_2000|Compound:st075254|FD-Score:3.09|P-value:3.93E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.39|P-value:8.75E-7 ID:SGTC_2110|Compound:5483698|FD-Score:-2.92|P-value:7.31E-4 ID:SGTC_2315|Compound:7733312|FD-Score:2.94|P-value:6.95E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.52|P-value:6.42E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.43|P-value:7.00E-7 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.25|P-value:2.07E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:2.85|P-value:9.66E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:7.11|P-value:5.04E-15 ID:SGTC_2692|Compound:st077426|FD-Score:3.05|P-value:4.58E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.93|P-value:9.53E-6 ID:SGTC_2806|Compound:7952793|FD-Score:-2.90|P-value:7.85E-4 ID:SGTC_3012|Compound:9080433|FD-Score:2.90|P-value:8.02E-4 ID:SGTC_3179|Compound:9107801|FD-Score:2.99|P-value:5.63E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.41|P-value:1.04E-4

Top fitness defect scores for YMR013C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2687 chenodiol 7.11 100.0 µM 5.04E-15 1 5.77E-13 2.91
2 SGTC_452 diindolylmethane 4.90 8.0 µM 4.87E-8 RNA processing & uracil transport 2 4.80E-7 0.68
3 SGTC_2413 inermin 4.43 26.1 µM 7.00E-7 13 4.61E-6 0.19
4 SGTC_2021 4019055 4.39 173.0 µM 8.75E-7 endomembrane recycling 30 5.57E-6 0.40
5 SGTC_1459 k035-0031 4.35 35.7 µM 1.11E-6 DNA intercalators 10 6.84E-6 0.13
6 SGTC_2693 st077581 3.93 40.7 µM 9.53E-6 6 4.25E-5 0.24
7 SGTC_416 estradiol valerate 3.87 100.0 µM 1.26E-5 5 5.39E-5 0.10
8 SGTC_1871 st057448 3.60 67.3 µM 4.44E-5 13 1.58E-4 0.03
9 SGTC_568 1193-0097 3.59 7.5 µM 4.75E-5 ERAD & cell cycle 29 1.67E-4 0.11
10 SGTC_2363 9053361 3.52 176.0 µM 6.42E-5 Golgi 15 2.16E-4 0.08
11 SGTC_428 3448-4153 3.51 16.5 µM 6.67E-5 TSC3-RPN4 12 2.23E-4 0.06
12 SGTC_1926 methyl fluorone black 3.45 53.6 µM 8.63E-5 DNA intercalators 35 2.78E-4 0.05
13 SGTC_1161 k007-0610 3.44 110.0 µM 8.97E-5 ERAD & cell cycle 46 2.87E-4 0.04
14 SGTC_3328 9140624 3.41 29.0 µM 1.04E-4 8 3.26E-4 0.09
15 SGTC_203 k066-0004 3.34 30.9 µM 1.41E-4 RSC & ERG11 19 4.22E-4 0.10
16 SGTC_522 1273-0010 3.27 11.6 µM 1.86E-4 60S ribosome export 15 5.34E-4 0.25
17 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 3.25 100.0 µM 2.07E-4 13 5.86E-4 0.02
18 SGTC_345 1362-0200 3.22 395.0 µM 2.27E-4 5 6.34E-4 0.01
19 SGTC_919 tnp00227 3.18 403.6 nM 2.74E-4 RNA pol III & RNase P/MRP 18 7.44E-4 0.08
20 SGTC_412 gemfibrozil 3.15 100.0 µM 3.06E-4 DNA intercalators 17 8.18E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 2.79E-21 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.159 2.10E-20 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.149 4.29E-18 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.135 4.88E-15 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.134 6.71E-15 YGL113W SLD3 het Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.120 3.36E-12 YDR458C HEH2 hom Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
0.120 3.24E-12 YLR035C MLH2 hom Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p
0.116 1.43E-11 YDL138W RGT2 hom Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
0.116 1.53E-11 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.114 3.30E-11 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.114 3.53E-11 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.113 5.35E-11 YDR344C_d YDR344C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.112 9.51E-11 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.111 1.17E-10 YBR297W MAL33 hom MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C
0.110 1.65E-10 YKR027W BCH2 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication